##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AclI AgeI Alw44I ApaLI AscI
AviII BbrPI BsiWI BsmBI BspDI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII EcoNI EcoRI EcoRV FspI
HpaI MfeI MluI MunI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
StuI SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1965 GACGTC GACGT^C
Acc65I 3314 GGTACC G^GTACC
AccIII 723 TCCGGA T^CCGGA
AflII 4118 CTTAAG C^TTAAG
AhdI 4008 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1700 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1512 AGCGCT AGC^GCT
ApaI 384 GGGCCC GGGCC^C
AseI 4913 ATTAAT AT^TAAT
AsnI 4913 ATTAAT AT^TAAT
Asp700I 1044 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3314 GGTACC G^GTACC
AspEI 4008 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2784 GGATCC G^GATCC
BbvCI 480 CCTCAGC CCTCAGC(-5/-2)
BcgI 223 CGATGGCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2270 GTATCC GTATCC(6/5)
BfrI 4118 CTTAAG C^TTAAG
BsaI 138 GAGACC GGTCTC(1/5)
BsaWI 723 TCCGGA [AT]^CCGG[AT]
BseAI 723 TCCGGA T^CCGGA
BspEI 723 TCCGGA T^CCGGA
BssSI 465 CACGAG CACGAG(-5/-1)
BstAPI 401 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2489 GGTCACC G^GT[ACGT]ACC
BstPI 2489 GGTCACC G^GT[ACGT]ACC
Bsu36I 1700 CCTAAGG CC^T[ACGT]AGG
Eam1105I 4008 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1512 AGCGCT AGC^GCT
Eco81I 1700 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2489 GGTCACC G^GT[ACGT]ACC
FseI 666 GGCCGGCC GGCCGG^CC
KpnI 3314 GGTACC GGTAC^C
KspI 432 CCGCGG CCGC^GG
MroI 723 TCCGGA T^CCGGA
NcoI 246 CCATGG C^CATGG
NdeI 2496 CATATG CA^TATG
PshBI 4913 ATTAAT AT^TAAT
SacII 432 CCGCGG CCGC^GG
SalI 915 GTCGAC G^TCGAC
SapI 4703 GCTCTTC GCTCTTC(1/4)
SbfI 1727 CCTGCAGG CCTGCA^GG
ScaI 2796 AGTACT AGT^ACT
Sse8387I 1727 CCTGCAGG CCTGCA^GG
XmnI 1044 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 915 GTCGAC GT^[AC][GT]AC
1020 GTCTAC
AvrII 163 CCTAGG C^CTAGG
3764 CCTAGG
BalI 872 TGGCCA TGG^CCA
2219 TGGCCA
BbeI 497 GGCGCC GGCGC^C
1903 GGCGCC
BglII 1468 AGATCT A^GATCT
2654 AGATCT
BlnI 163 CCTAGG C^CTAGG
3764 CCTAGG
BsaAI 744 TACGTG [CT]AC^GT[GA]
2159 TACGTG
BsaBI 2649 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3775 GATTGGAATC
BsgI 2278 GTGCAG GTGCAG(16/14)
2605 GTGCAG
BsmI 1221 GAATGC GAATGC(1/-1)
1754 GAATGC
BspHI 949 TCATGA T^CATGA
1519 TCATGA
BspLU11I 3131 ACATGT A^CATGT
4502 ACATGT
BsrBI 371 CCGCTC CCGCTC(-3/-3)
37 GAGCGG
BsrDI 2365 CATTGC GCAATG(2/0)
4478 CATTGC
BsrFI 539 GCCGGC [GA]^CCGG[CT]
667 GCCGGC
BstXI 2812 CCATGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4513 CCAGACTGATGG
Cfr10I 539 GCCGGC [GA]^CCGG[CT]
667 GCCGGC
DraI 3435 TTTAAA TTT^AAA
3488 TTTAAA
DrdI 1011 GACGACGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2481 GACCCAAAGGTC
EcoT22I 2579 ATGCAT ATGCA^T
3751 ATGCAT
KasI 497 GGCGCC G^GCGCC
1903 GGCGCC
MamI 2649 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3775 GATTGGAATC
MluNI 872 TGGCCA TGG^CCA
2219 TGGCCA
MscI 872 TGGCCA TGG^CCA
2219 TGGCCA
NaeI 539 GCCGGC GCC^GGC
667 GCCGGC
NarI 497 GGCGCC GG^CGCC
1903 GGCGCC
NgoMIV 539 GCCGGC G^CCGGC
667 GCCGGC
NsiI 2579 ATGCAT ATGCA^T
3751 ATGCAT
RcaI 949 TCATGA T^CATGA
1519 TCATGA
SfoI 497 GGCGCC GGC^GCC
1903 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmaCI XmaI