################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AclI AgeI Alw44I ApaLI AscI AviII BbrPI BsiWI BsmBI BspDI Bst1107I BstBI BstZ17I ClaI CpoI DraIII EcoNI EcoRI EcoRV FspI HpaI MfeI MluI MunI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SexAI SfiI SfuI SgrAI SnaBI SpeI SphI StuI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 1965 GACGTC GACGT^C Acc65I 3314 GGTACC G^GTACC AccIII 723 TCCGGA T^CCGGA AflII 4118 CTTAAG C^TTAAG AhdI 4008 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1700 CCTAAGG CC^T[ACGT]AGG Aor51HI 1512 AGCGCT AGC^GCT ApaI 384 GGGCCC GGGCC^C AseI 4913 ATTAAT AT^TAAT AsnI 4913 ATTAAT AT^TAAT Asp700I 1044 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3314 GGTACC G^GTACC AspEI 4008 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2784 GGATCC G^GATCC BbvCI 480 CCTCAGC CCTCAGC(-5/-2) BcgI 223 CGATGGCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2270 GTATCC GTATCC(6/5) BfrI 4118 CTTAAG C^TTAAG BsaI 138 GAGACC GGTCTC(1/5) BsaWI 723 TCCGGA [AT]^CCGG[AT] BseAI 723 TCCGGA T^CCGGA BspEI 723 TCCGGA T^CCGGA BssSI 465 CACGAG CACGAG(-5/-1) BstAPI 401 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2489 GGTCACC G^GT[ACGT]ACC BstPI 2489 GGTCACC G^GT[ACGT]ACC Bsu36I 1700 CCTAAGG CC^T[ACGT]AGG Eam1105I 4008 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1512 AGCGCT AGC^GCT Eco81I 1700 CCTAAGG CC^T[ACGT]AGG EcoO65I 2489 GGTCACC G^GT[ACGT]ACC FseI 666 GGCCGGCC GGCCGG^CC KpnI 3314 GGTACC GGTAC^C KspI 432 CCGCGG CCGC^GG MroI 723 TCCGGA T^CCGGA NcoI 246 CCATGG C^CATGG NdeI 2496 CATATG CA^TATG PshBI 4913 ATTAAT AT^TAAT SacII 432 CCGCGG CCGC^GG SalI 915 GTCGAC G^TCGAC SapI 4703 GCTCTTC GCTCTTC(1/4) SbfI 1727 CCTGCAGG CCTGCA^GG ScaI 2796 AGTACT AGT^ACT Sse8387I 1727 CCTGCAGG CCTGCA^GG XmnI 1044 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 915 GTCGAC GT^[AC][GT]AC 1020 GTCTAC AvrII 163 CCTAGG C^CTAGG 3764 CCTAGG BalI 872 TGGCCA TGG^CCA 2219 TGGCCA BbeI 497 GGCGCC GGCGC^C 1903 GGCGCC BglII 1468 AGATCT A^GATCT 2654 AGATCT BlnI 163 CCTAGG C^CTAGG 3764 CCTAGG BsaAI 744 TACGTG [CT]AC^GT[GA] 2159 TACGTG BsaBI 2649 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3775 GATTGGAATC BsgI 2278 GTGCAG GTGCAG(16/14) 2605 GTGCAG BsmI 1221 GAATGC GAATGC(1/-1) 1754 GAATGC BspHI 949 TCATGA T^CATGA 1519 TCATGA BspLU11I 3131 ACATGT A^CATGT 4502 ACATGT BsrBI 371 CCGCTC CCGCTC(-3/-3) 37 GAGCGG BsrDI 2365 CATTGC GCAATG(2/0) 4478 CATTGC BsrFI 539 GCCGGC [GA]^CCGG[CT] 667 GCCGGC BstXI 2812 CCATGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4513 CCAGACTGATGG Cfr10I 539 GCCGGC [GA]^CCGG[CT] 667 GCCGGC DraI 3435 TTTAAA TTT^AAA 3488 TTTAAA DrdI 1011 GACGACGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2481 GACCCAAAGGTC EcoT22I 2579 ATGCAT ATGCA^T 3751 ATGCAT KasI 497 GGCGCC G^GCGCC 1903 GGCGCC MamI 2649 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3775 GATTGGAATC MluNI 872 TGGCCA TGG^CCA 2219 TGGCCA MscI 872 TGGCCA TGG^CCA 2219 TGGCCA NaeI 539 GCCGGC GCC^GGC 667 GCCGGC NarI 497 GGCGCC GG^CGCC 1903 GGCGCC NgoMIV 539 GCCGGC G^CCGGC 667 GCCGGC NsiI 2579 ATGCAT ATGCA^T 3751 ATGCAT RcaI 949 TCATGA T^CATGA 1519 TCATGA SfoI 497 GGCGCC GGC^GCC 1903 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII XmaCI XmaI