################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI Eam1105I EcoRI EcoRV FseI FspI HindIII HpaI KasI KpnI KspI MamI MluI NaeI NarI NcoI NgoMIV NheI NruI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuII RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 2459 CGCG CG^CG AccIII 950 TCCGGA T^CCGGA AocI 2255 CCTGAGG CC^T[ACGT]AGG Aor51HI 2583 AGCGCT AGC^GCT ApaI 2002 GGGCCC GGGCC^C BbvCI 867 CCTCAGC CCTCAGC(-5/-2) BsaAI 3515 TACGTG [CT]AC^GT[GA] BseAI 950 TCCGGA T^CCGGA BspEI 950 TCCGGA T^CCGGA BstAPI 263 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1485 GGTTACC G^GT[ACGT]ACC BstPI 1485 GGTTACC G^GT[ACGT]ACC BstUI 2459 CGCG CG^CG BstXI 882 CCAGAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2255 CCTGAGG CC^T[ACGT]AGG DraIII 3452 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1901 CCACGG C^C[GA][CT]GG Eco47III 2583 AGCGCT AGC^GCT Eco81I 2255 CCTGAGG CC^T[ACGT]AGG EcoO65I 1485 GGTTACC G^GT[ACGT]ACC HaeII 2583 AGCGCT [GA]GCGC^[CT] MfeI 3648 CAATTG C^AATTG MroI 950 TCCGGA T^CCGGA MunI 3648 CAATTG C^AATTG MvnI 2459 CGCG CG^CG NdeI 3978 CATATG CA^TATG NotI 3274 GCGGCCGC GC^GGCCGC PaeR7I 150 CTCGAG C^TCGAG PshAI 2032 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1585 CGATCG CGAT^CG SacI 2519 GAGCTC GAGCT^C SalI 290 GTCGAC G^TCGAC SapI 1802 GAAGAGC GCTCTTC(1/4) SmaI 2883 CCCGGG CCC^GGG StuI 1652 AGGCCT AGG^CCT XbaI 3206 TCTAGA T^CTAGA XcmI 224 CCAGCCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 150 CTCGAG C^TCGAG XmaCI 2883 CCCGGG C^CCGGG XmaI 2883 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 1437 GACGCC G[GA]^CG[CT]C 1938 GACGCC AlwNI 1701 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1818 CAGCCACTG AseI 41 ATTAAT AT^TAAT 2273 ATTAAT AsnI 41 ATTAAT AT^TAAT 2273 ATTAAT Asp700I 134 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1226 GAAAATCTTC BalI 1336 TGGCCA TGG^CCA 1610 TGGCCA BcgI 1127 CGATGGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2680 GCACAACCTTCG BclI 244 TGATCA T^GATCA 3262 TGATCA BglII 3543 AGATCT A^GATCT 3715 AGATCT BsaHI 1437 GACGCC G[GA]^CG[CT]C 1938 GACGCC BspMI 1931 ACCTGC ACCTGC(4/8) 3686 GCAGGT BsrBI 158 GAGCGG CCGCTC(-3/-3) 3392 GAGCGG BssSI 2319 CTCGTG CACGAG(-5/-1) 3699 CTCGTG DrdI 3309 GACGGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3665 GACTATACGGTC EagI 2249 CGGCCG C^GGCCG 3275 CGGCCG EarI 885 GAAGAG CTCTTC(1/4) 1802 GAAGAG EclXI 2249 CGGCCG C^GGCCG 3275 CGGCCG Eco52I 2249 CGGCCG C^GGCCG 3275 CGGCCG EcoNI 2049 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2465 CCTGACGGAGG EcoT22I 904 ATGCAT ATGCA^T 2695 ATGCAT FbaI 244 TGATCA T^GATCA 3262 TGATCA Hin1I 1437 GACGCC G[GA]^CG[CT]C 1938 GACGCC Ksp632I 885 GAAGAG CTCTTC(1/4) 1802 GAAGAG MluNI 1336 TGGCCA TGG^CCA 1610 TGGCCA MscI 1336 TGGCCA TGG^CCA 1610 TGGCCA MslI 1206 CACACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1907 CATACAGGTG NsiI 904 ATGCAT ATGCA^T 2695 ATGCAT PshBI 41 ATTAAT AT^TAAT 2273 ATTAAT SmlI 150 CTCGAG C^T[CT][GA]AG 1216 CTCAAG SspI 3813 AATATT AAT^ATT 4158 AATATT XmnI 134 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1226 GAAAATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AspHI AspI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII