##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstZ17I CelII Cfr10I ClaI CpoI
Eam1105I EcoRI EcoRV FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MluI NaeI NarI NcoI
NgoMIV NheI NruI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuII RcaI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 2459 CGCG CG^CG
AccIII 950 TCCGGA T^CCGGA
AocI 2255 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2583 AGCGCT AGC^GCT
ApaI 2002 GGGCCC GGGCC^C
BbvCI 867 CCTCAGC CCTCAGC(-5/-2)
BsaAI 3515 TACGTG [CT]AC^GT[GA]
BseAI 950 TCCGGA T^CCGGA
BspEI 950 TCCGGA T^CCGGA
BstAPI 263 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1485 GGTTACC G^GT[ACGT]ACC
BstPI 1485 GGTTACC G^GT[ACGT]ACC
BstUI 2459 CGCG CG^CG
BstXI 882 CCAGAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2255 CCTGAGG CC^T[ACGT]AGG
DraIII 3452 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1901 CCACGG C^C[GA][CT]GG
Eco47III 2583 AGCGCT AGC^GCT
Eco81I 2255 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1485 GGTTACC G^GT[ACGT]ACC
HaeII 2583 AGCGCT [GA]GCGC^[CT]
MfeI 3648 CAATTG C^AATTG
MroI 950 TCCGGA T^CCGGA
MunI 3648 CAATTG C^AATTG
MvnI 2459 CGCG CG^CG
NdeI 3978 CATATG CA^TATG
NotI 3274 GCGGCCGC GC^GGCCGC
PaeR7I 150 CTCGAG C^TCGAG
PshAI 2032 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1585 CGATCG CGAT^CG
SacI 2519 GAGCTC GAGCT^C
SalI 290 GTCGAC G^TCGAC
SapI 1802 GAAGAGC GCTCTTC(1/4)
SmaI 2883 CCCGGG CCC^GGG
StuI 1652 AGGCCT AGG^CCT
XbaI 3206 TCTAGA T^CTAGA
XcmI 224 CCAGCCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 150 CTCGAG C^TCGAG
XmaCI 2883 CCCGGG C^CCGGG
XmaI 2883 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1437 GACGCC G[GA]^CG[CT]C
1938 GACGCC
AlwNI 1701 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1818 CAGCCACTG
AseI 41 ATTAAT AT^TAAT
2273 ATTAAT
AsnI 41 ATTAAT AT^TAAT
2273 ATTAAT
Asp700I 134 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1226 GAAAATCTTC
BalI 1336 TGGCCA TGG^CCA
1610 TGGCCA
BcgI 1127 CGATGGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2680 GCACAACCTTCG
BclI 244 TGATCA T^GATCA
3262 TGATCA
BglII 3543 AGATCT A^GATCT
3715 AGATCT
BsaHI 1437 GACGCC G[GA]^CG[CT]C
1938 GACGCC
BspMI 1931 ACCTGC ACCTGC(4/8)
3686 GCAGGT
BsrBI 158 GAGCGG CCGCTC(-3/-3)
3392 GAGCGG
BssSI 2319 CTCGTG CACGAG(-5/-1)
3699 CTCGTG
DrdI 3309 GACGGCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3665 GACTATACGGTC
EagI 2249 CGGCCG C^GGCCG
3275 CGGCCG
EarI 885 GAAGAG CTCTTC(1/4)
1802 GAAGAG
EclXI 2249 CGGCCG C^GGCCG
3275 CGGCCG
Eco52I 2249 CGGCCG C^GGCCG
3275 CGGCCG
EcoNI 2049 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2465 CCTGACGGAGG
EcoT22I 904 ATGCAT ATGCA^T
2695 ATGCAT
FbaI 244 TGATCA T^GATCA
3262 TGATCA
Hin1I 1437 GACGCC G[GA]^CG[CT]C
1938 GACGCC
Ksp632I 885 GAAGAG CTCTTC(1/4)
1802 GAAGAG
MluNI 1336 TGGCCA TGG^CCA
1610 TGGCCA
MscI 1336 TGGCCA TGG^CCA
1610 TGGCCA
MslI 1206 CACACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1907 CATACAGGTG
NsiI 904 ATGCAT ATGCA^T
2695 ATGCAT
PshBI 41 ATTAAT AT^TAAT
2273 ATTAAT
SmlI 150 CTCGAG C^T[CT][GA]AG
1216 CTCAAG
SspI 3813 AATATT AAT^ATT
4158 AATATT
XmnI 134 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1226 GAAAATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AspI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII