##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
AscI Asp718I AspEI BciVI BclI
BfrI BglI BlpI Bpu1102I BsaBI
BsaI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssSI Bst1107I BstBI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoT22I FbaI FseI HpaI KpnI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmeI PvuI
RcaI RsrII SapI SbfI SexAI
SfiI SfuI SgrAI SpeI SphI
Sse8387I SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 188 GTCGAC GT^[AC][GT]AC
AclI 3435 AACGTT AA^CGTT
Alw44I 2416 GTGCAC G^TGCAC
AocI 1562 CCTCAGG CC^T[ACGT]AGG
ApaLI 2416 GTGCAC G^TGCAC
AseI 3152 ATTAAT AT^TAAT
AsnI 3152 ATTAAT AT^TAAT
AspI 196 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1095 TGCGCA TGC^GCA
AvrII 440 CCTAGG C^CTAGG
BcgI 952 CGACATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2124 AGATCT A^GATCT
BlnI 440 CCTAGG C^CTAGG
BseRI 1763 GAGGAG GAGGAG(10/8)
BsmI 88 GCATTC GAATGC(1/-1)
BsrDI 2888 GCAATG GCAATG(2/0)
BssHII 251 GCGCGC G^CGCGC
BstEII 272 GGTCACC G^GT[ACGT]ACC
BstPI 272 GGTCACC G^GT[ACGT]ACC
Bsu36I 1562 CCTCAGG CC^T[ACGT]AGG
Eco81I 1562 CCTCAGG CC^T[ACGT]AGG
EcoO65I 272 GGTCACC G^GT[ACGT]ACC
EcoRV 1907 GATATC GAT^ATC
FspI 1095 TGCGCA TGC^GCA
HindIII 3312 AAGCTT A^AGCTT
KspI 232 CCGCGG CCGC^GG
NcoI 1572 CCATGG C^CATGG
PflFI 196 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1980 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3152 ATTAAT AT^TAAT
Psp1406I 3435 AACGTT AA^CGTT
PvuII 1113 CAGCTG CAG^CTG
SacII 232 CCGCGG CCGC^GG
SalI 188 GTCGAC G^TCGAC
ScaI 3382 AGTACT AGT^ACT
SnaBI 3045 TACGTA TAC^GTA
SspI 2283 AATATT AAT^ATT
Tth111I 196 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 299 GACGTC GACGT^C
1823 GACGTC
AflIII 180 ACACGT A^C[GA][CT]GT
2469 ACGTGT
Aor51HI 1328 AGCGCT AGC^GCT
2724 AGCGCT
Asp700I 1950 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3120 GAAAAGTTTC
BbrPI 2468 CACGTG CAC^GTG
2696 CACGTG
BsaWI 1010 TCCGGT [AT]^CCGG[AT]
2761 TCCGGT
BsiEI 186 CGGTCG CG[GA][CT]^CG
2407 CGGTCG
Bsp1407I 2160 TGTACA T^GTACA
2523 TGTACA
BspMI 888 ACCTGC ACCTGC(4/8)
2599 GCAGGT
BsrGI 2160 TGTACA T^GTACA
2523 TGTACA
BstAPI 1600 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2246 GCAGAAGTTGC
DsaI 232 CCGCGG C^C[GA][CT]GG
1572 CCATGG
Eco47III 1328 AGCGCT AGC^GCT
2724 AGCGCT
HincII 188 GTCGAC GT[CT]^[GA]AC
1064 GTCAAC
HindII 188 GTCGAC GT[CT]^[GA]AC
1064 GTCAAC
PmaCI 2468 CACGTG CAC^GTG
2696 CACGTG
PmlI 2468 CACGTG CAC^GTG
2696 CACGTG
PpuMI 837 AGGACCC [GA]G^G[AT]CC[CT]
1482 AGGTCCT
SmaI 384 CCCGGG CCC^GGG
712 CCCGGG
SmlI 430 CTCAAG C^T[CT][GA]AG
769 CTTGAG
SspBI 2160 TGTACA T^GTACA
2523 TGTACA
Tsp45I 273 GTCAC ^GT[GC]AC
666 GTCAC
XcmI 1512 CCATCACCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1518 CCATTATGGGCCTGG
XmaCI 384 CCCGGG C^CCGGG
712 CCCGGG
XmaI 384 CCCGGG C^CCGGG
712 CCCGGG
XmnI 1950 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3120 GAAAAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StuI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII