################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## Acc65I AccIII AflII AgeI AhdI AscI Asp718I AspEI BciVI BclI BfrI BglI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssSI Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoT22I FbaI FseI HpaI KpnI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PvuI RcaI RsrII SapI SbfI SexAI SfiI SfuI SgrAI SpeI SphI Sse8387I SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 188 GTCGAC GT^[AC][GT]AC AclI 3435 AACGTT AA^CGTT Alw44I 2416 GTGCAC G^TGCAC AocI 1562 CCTCAGG CC^T[ACGT]AGG ApaLI 2416 GTGCAC G^TGCAC AseI 3152 ATTAAT AT^TAAT AsnI 3152 ATTAAT AT^TAAT AspI 196 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1095 TGCGCA TGC^GCA AvrII 440 CCTAGG C^CTAGG BcgI 952 CGACATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2124 AGATCT A^GATCT BlnI 440 CCTAGG C^CTAGG BseRI 1763 GAGGAG GAGGAG(10/8) BsmI 88 GCATTC GAATGC(1/-1) BsrDI 2888 GCAATG GCAATG(2/0) BssHII 251 GCGCGC G^CGCGC BstEII 272 GGTCACC G^GT[ACGT]ACC BstPI 272 GGTCACC G^GT[ACGT]ACC Bsu36I 1562 CCTCAGG CC^T[ACGT]AGG Eco81I 1562 CCTCAGG CC^T[ACGT]AGG EcoO65I 272 GGTCACC G^GT[ACGT]ACC EcoRV 1907 GATATC GAT^ATC FspI 1095 TGCGCA TGC^GCA HindIII 3312 AAGCTT A^AGCTT KspI 232 CCGCGG CCGC^GG NcoI 1572 CCATGG C^CATGG PflFI 196 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1980 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3152 ATTAAT AT^TAAT Psp1406I 3435 AACGTT AA^CGTT PvuII 1113 CAGCTG CAG^CTG SacII 232 CCGCGG CCGC^GG SalI 188 GTCGAC G^TCGAC ScaI 3382 AGTACT AGT^ACT SnaBI 3045 TACGTA TAC^GTA SspI 2283 AATATT AAT^ATT Tth111I 196 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AatII 299 GACGTC GACGT^C 1823 GACGTC AflIII 180 ACACGT A^C[GA][CT]GT 2469 ACGTGT Aor51HI 1328 AGCGCT AGC^GCT 2724 AGCGCT Asp700I 1950 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3120 GAAAAGTTTC BbrPI 2468 CACGTG CAC^GTG 2696 CACGTG BsaWI 1010 TCCGGT [AT]^CCGG[AT] 2761 TCCGGT BsiEI 186 CGGTCG CG[GA][CT]^CG 2407 CGGTCG Bsp1407I 2160 TGTACA T^GTACA 2523 TGTACA BspMI 888 ACCTGC ACCTGC(4/8) 2599 GCAGGT BsrGI 2160 TGTACA T^GTACA 2523 TGTACA BstAPI 1600 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2246 GCAGAAGTTGC DsaI 232 CCGCGG C^C[GA][CT]GG 1572 CCATGG Eco47III 1328 AGCGCT AGC^GCT 2724 AGCGCT HincII 188 GTCGAC GT[CT]^[GA]AC 1064 GTCAAC HindII 188 GTCGAC GT[CT]^[GA]AC 1064 GTCAAC PmaCI 2468 CACGTG CAC^GTG 2696 CACGTG PmlI 2468 CACGTG CAC^GTG 2696 CACGTG PpuMI 837 AGGACCC [GA]G^G[AT]CC[CT] 1482 AGGTCCT SmaI 384 CCCGGG CCC^GGG 712 CCCGGG SmlI 430 CTCAAG C^T[CT][GA]AG 769 CTTGAG SspBI 2160 TGTACA T^GTACA 2523 TGTACA Tsp45I 273 GTCAC ^GT[GC]AC 666 GTCAC XcmI 1512 CCATCACCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1518 CCATTATGGGCCTGG XmaCI 384 CCCGGG C^CCGGG 712 CCCGGG XmaI 384 CCCGGG C^CCGGG 712 CCCGGG XmnI 1950 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3120 GAAAAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StuI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII