##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccII AclI AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII BamHI BbrPI BciVI BclI
BglI BsaAI BsaBI BseRI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstUI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRV FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2575 GACGTC GACGT^C
AccIII 2581 TCCGGA T^CCGGA
AciI 2053 CCGC CCGC(-3/-1)
AflII 558 CTTAAG C^TTAAG
AflIII 445 ACGTGT A^C[GA][CT]GT
AocI 2395 CCTGAGG CC^T[ACGT]AGG
Aor51HI 237 AGCGCT AGC^GCT
BalI 3183 TGGCCA TGG^CCA
BbeI 3332 GGCGCC GGCGC^C
BcgI 1668 GCACAGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4168 CCCGG CC^[GC]GG
BfrI 558 CTTAAG C^TTAAG
BsaI 2048 GAGACC GGTCTC(1/5)
BsaWI 2581 TCCGGA [AT]^CCGG[AT]
BseAI 2581 TCCGGA T^CCGGA
BsgI 397 CTGCAC GTGCAG(16/14)
BsmBI 2577 CGTCTC CGTCTC(1/5)
BspEI 2581 TCCGGA T^CCGGA
BsrDI 1129 GCAATG GCAATG(2/0)
BstXI 4340 CCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2395 CCTGAGG CC^T[ACGT]AGG
DraIII 3661 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3183 TGGCCA [CT]^GGCC[GA]
Eco47III 237 AGCGCT AGC^GCT
Eco81I 2395 CCTGAGG CC^T[ACGT]AGG
FauI 2052 CCCGC CCCGC(4/6)
HincII 2486 GTCAAC GT[CT]^[GA]AC
HindII 2486 GTCAAC GT[CT]^[GA]AC
HindIII 1753 AAGCTT A^AGCTT
KasI 3332 GGCGCC G^GCGCC
MluNI 3183 TGGCCA TGG^CCA
MroI 2581 TCCGGA T^CCGGA
MscI 3183 TGGCCA TGG^CCA
MspA1I 1878 CAGCTG C[AC]G^C[GT]G
NarI 3332 GGCGCC GG^CGCC
NciI 4168 CCCGG CC^[GC]GG
NdeI 3967 CATATG CA^TATG
NheI 148 GCTAGC G^CTAGC
PvuII 1878 CAGCTG CAG^CTG
ScaI 2334 AGTACT AGT^ACT
SfoI 3332 GGCGCC GGC^GCC
SmlI 558 CTTAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 2575 GACGTC G[GA]^CG[CT]C
3332 GGCGCC
BanI 215 GGCACC G^G[CT][GA]CC
3332 GGCGCC
BglII 2403 AGATCT A^GATCT
3635 AGATCT
BlpI 1411 GCTCAGC GC^T[ACGT]AGC
3446 GCTGAGC
Bpu1102I 1411 GCTCAGC GC^T[ACGT]AGC
3446 GCTGAGC
BsaHI 2575 GACGTC G[GA]^CG[CT]C
3332 GGCGCC
BspMI 125 ACCTGC ACCTGC(4/8)
4118 ACCTGC
Bst1107I 2091 GTATAC GTA^TAC
4329 GTATAC
BstAPI 198 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3287 GCACTTGTTGC
BstEII 338 GGTGACC G^GT[ACGT]ACC
3931 GGTCACC
BstPI 338 GGTGACC G^GT[ACGT]ACC
3931 GGTCACC
BstZ17I 2091 GTATAC GTA^TAC
4329 GTATAC
CelII 1411 GCTCAGC GC^T[ACGT]AGC
3446 GCTGAGC
DraI 2448 TTTAAA TTT^AAA
3560 TTTAAA
DsaI 184 CCATGG C^C[GA][CT]GG
3134 CCATGG
EcoO65I 338 GGTGACC G^GT[ACGT]ACC
3931 GGTCACC
EcoT22I 205 ATGCAT ATGCA^T
3874 ATGCAT
HaeII 237 AGCGCT [GA]GCGC^[CT]
3332 GGCGCC
HgaI 386 GCGTC GACGC(5/10)
1564 GCGTC
Hin1I 2575 GACGTC G[GA]^CG[CT]C
3332 GGCGCC
NcoI 184 CCATGG C^CATGG
3134 CCATGG
NsiI 205 ATGCAT ATGCA^T
3874 ATGCAT
PshAI 2434 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3950 GACAAGAGTC
SapI 1816 GAAGAGC GCTCTTC(1/4)
3699 GAAGAGC
SphI 3876 GCATGC GCATG^C
4152 GCATGC
SspI 2154 AATATT AAT^ATT
3233 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
AvrII BanII BbsI BbvCI BbvI
BfaI BlnI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI