################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspI AviII AvrII BalI BamHI BbeI BlnI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrDI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HpaI KasI KspI MluI MluNI MroI MscI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 3611 GGTACC G^GTACC AflII 4239 CTTAAG C^TTAAG AflIII 4361 ACACGT A^C[GA][CT]GT AseI 1124 ATTAAT AT^TAAT AsnI 1124 ATTAAT AT^TAAT Asp718I 3611 GGTACC G^GTACC AvaI 3552 CCCGAG C^[CT]CG[GA]G BanI 3611 GGTACC G^G[CT][GA]CC BbrPI 4362 CACGTG CAC^GTG BbvCI 917 GCTGAGG CCTCAGC(-5/-2) BcgI 3730 GCACATTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2613 TGATCA T^GATCA BfrI 4239 CTTAAG C^TTAAG BglI 1711 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1509 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4351 TCCGGT [AT]^CCGG[AT] BsmBI 383 GAGACG CGTCTC(1/5) BsoBI 3552 CCCGAG C^[CT]CG[GA]G BstAPI 3122 GCACAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EaeI 621 CGGCCA [CT]^GGCC[GA] EarI 2425 GAAGAG CTCTTC(1/4) EcoT22I 933 ATGCAT ATGCA^T FauI 4344 CCCGC CCCGC(4/6) FbaI 2613 TGATCA T^GATCA HaeII 1465 GGCGCT [GA]GCGC^[CT] KpnI 3611 GGTACC GGTAC^C Ksp632I 2425 GAAGAG CTCTTC(1/4) MamI 1509 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 262 CATATG CA^TATG NsiI 933 ATGCAT ATGCA^T PmaCI 4362 CACGTG CAC^GTG PmlI 4362 CACGTG CAC^GTG PshBI 1124 ATTAAT AT^TAAT ScaI 2194 AGTACT AGT^ACT SnaBI 467 TACGTA TAC^GTA XcmI 2237 CCAGGATGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 3506 GTAGAC GT^[AC][GT]AC 3691 GTCTAC AccII 2 CGCG CG^CG 17 CGCG AcyI 147 GACGCC G[GA]^CG[CT]C 642 GGCGTC AocI 1065 CCTTAGG CC^T[ACGT]AGG 1743 CCTCAGG BciVI 464 GGATAC GTATCC(6/5) 3910 GTATCC BcnI 1221 CCCGG CC^[GC]GG 3587 CCCGG BlpI 2828 GCTCAGC GC^T[ACGT]AGC 3030 GCTGAGC Bpu1102I 2828 GCTCAGC GC^T[ACGT]AGC 3030 GCTGAGC BsaHI 147 GACGCC G[GA]^CG[CT]C 642 GGCGTC BsaI 1683 GGTCTC GGTCTC(1/5) 3840 GGTCTC BsmI 881 GCATTC GAATGC(1/-1) 4490 GCATTC Bsp1407I 1031 TGTACA T^GTACA 3756 TGTACA BsrBI 20 GAGCGG CCGCTC(-3/-3) 336 GAGCGG BsrGI 1031 TGTACA T^GTACA 3756 TGTACA BstUI 2 CGCG CG^CG 17 CGCG Bsu36I 1065 CCTTAGG CC^T[ACGT]AGG 1743 CCTCAGG CelII 2828 GCTCAGC GC^T[ACGT]AGC 3030 GCTGAGC DraIII 696 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG 3760 CACTAAGTG DrdI 33 GACGAGCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3424 GACATAAATGTC DsaI 713 CCATGG C^C[GA][CT]GG 2753 CCATGG Eco81I 1065 CCTTAGG CC^T[ACGT]AGG 1743 CCTCAGG EcoNI 3672 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4455 CCTGACCAAGG EcoRI 1111 GAATTC G^AATTC 2159 GAATTC Hin1I 147 GACGCC G[GA]^CG[CT]C 642 GGCGTC HindIII 962 AAGCTT A^AGCTT 3109 AAGCTT MfeI 574 CAATTG C^AATTG 4190 CAATTG MunI 574 CAATTG C^AATTG 4190 CAATTG MvnI 2 CGCG CG^CG 17 CGCG NciI 1221 CCCGG CC^[GC]GG 3587 CCCGG NcoI 713 CCATGG C^CATGG 2753 CCATGG PflMI 452 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1488 CCAGCACTTGG PvuII 81 CAGCTG CAG^CTG 249 CAGCTG SspBI 1031 TGTACA T^GTACA 3756 TGTACA SspI 1346 AATATT AAT^ATT 2513 AATATT Van91I 452 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1488 CCAGCACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstBI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI NspV PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI