##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BlnI        BseAI       BsiEI       
BsiWI       BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       FseI        
FspI        HpaI        KasI        KspI        MluI        
MluNI       MroI        MscI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SpeI        Sse8387I    
StuI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       3611  GGTACC               G^GTACC
AflII        4239  CTTAAG               C^TTAAG
AflIII       4361  ACACGT               A^C[GA][CT]GT
AseI         1124  ATTAAT               AT^TAAT
AsnI         1124  ATTAAT               AT^TAAT
Asp718I      3611  GGTACC               G^GTACC
AvaI         3552  CCCGAG               C^[CT]CG[GA]G
BanI         3611  GGTACC               G^G[CT][GA]CC
BbrPI        4362  CACGTG               CAC^GTG
BbvCI         917  GCTGAGG              CCTCAGC(-5/-2)
BcgI         3730  GCACATTGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2613  TGATCA               T^GATCA
BfrI         4239  CTTAAG               C^TTAAG
BglI         1711  GCCAAGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1509  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        4351  TCCGGT               [AT]^CCGG[AT]
BsmBI         383  GAGACG               CGTCTC(1/5)
BsoBI        3552  CCCGAG               C^[CT]CG[GA]G
BstAPI       3122  GCACAGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI          621  CGGCCA               [CT]^GGCC[GA]
EarI         2425  GAAGAG               CTCTTC(1/4)
EcoT22I       933  ATGCAT               ATGCA^T
FauI         4344  CCCGC                CCCGC(4/6)
FbaI         2613  TGATCA               T^GATCA
HaeII        1465  GGCGCT               [GA]GCGC^[CT]
KpnI         3611  GGTACC               GGTAC^C
Ksp632I      2425  GAAGAG               CTCTTC(1/4)
MamI         1509  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI          262  CATATG               CA^TATG
NsiI          933  ATGCAT               ATGCA^T
PmaCI        4362  CACGTG               CAC^GTG
PmlI         4362  CACGTG               CAC^GTG
PshBI        1124  ATTAAT               AT^TAAT
ScaI         2194  AGTACT               AGT^ACT
SnaBI         467  TACGTA               TAC^GTA
XcmI         2237  CCAGGATGAGTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI         3506  GTAGAC               GT^[AC][GT]AC
             3691  GTCTAC              
AccII           2  CGCG                 CG^CG
               17  CGCG                
AcyI          147  GACGCC               G[GA]^CG[CT]C
              642  GGCGTC              
AocI         1065  CCTTAGG              CC^T[ACGT]AGG
             1743  CCTCAGG             
BciVI         464  GGATAC               GTATCC(6/5)
             3910  GTATCC              
BcnI         1221  CCCGG                CC^[GC]GG
             3587  CCCGG               
BlpI         2828  GCTCAGC              GC^T[ACGT]AGC
             3030  GCTGAGC             
Bpu1102I     2828  GCTCAGC              GC^T[ACGT]AGC
             3030  GCTGAGC             
BsaHI         147  GACGCC               G[GA]^CG[CT]C
              642  GGCGTC              
BsaI         1683  GGTCTC               GGTCTC(1/5)
             3840  GGTCTC              
BsmI          881  GCATTC               GAATGC(1/-1)
             4490  GCATTC              
Bsp1407I     1031  TGTACA               T^GTACA
             3756  TGTACA              
BsrBI          20  GAGCGG               CCGCTC(-3/-3)
              336  GAGCGG              
BsrGI        1031  TGTACA               T^GTACA
             3756  TGTACA              
BstUI           2  CGCG                 CG^CG
               17  CGCG                
Bsu36I       1065  CCTTAGG              CC^T[ACGT]AGG
             1743  CCTCAGG             
CelII        2828  GCTCAGC              GC^T[ACGT]AGC
             3030  GCTGAGC             
DraIII        696  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3760  CACTAAGTG           
DrdI           33  GACGAGCGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3424  GACATAAATGTC        
DsaI          713  CCATGG               C^C[GA][CT]GG
             2753  CCATGG              
Eco81I       1065  CCTTAGG              CC^T[ACGT]AGG
             1743  CCTCAGG             
EcoNI        3672  CCTTCTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4455  CCTGACCAAGG         
EcoRI        1111  GAATTC               G^AATTC
             2159  GAATTC              
Hin1I         147  GACGCC               G[GA]^CG[CT]C
              642  GGCGTC              
HindIII       962  AAGCTT               A^AGCTT
             3109  AAGCTT              
MfeI          574  CAATTG               C^AATTG
             4190  CAATTG              
MunI          574  CAATTG               C^AATTG
             4190  CAATTG              
MvnI            2  CGCG                 CG^CG
               17  CGCG                
NciI         1221  CCCGG                CC^[GC]GG
             3587  CCCGG               
NcoI          713  CCATGG               C^CATGG
             2753  CCATGG              
PflMI         452  CCAAGTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1488  CCAGCACTTGG         
PvuII          81  CAGCTG               CAG^CTG
              249  CAGCTG              
SspBI        1031  TGTACA               T^GTACA
             3756  TGTACA              
SspI         1346  AATATT               AAT^ATT
             2513  AATATT              
Van91I        452  CCAAGTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1488  CCAGCACTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BanII       BbsI        BbvI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstBI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
NspV        PleI        PpuMI       PstI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfuI        SmlI        SphI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI