##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspI AviII AvrII BalI
BamHI BbeI BlnI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HpaI KasI KspI MluI
MluNI MroI MscI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SpeI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 3611 GGTACC G^GTACC
AflII 4239 CTTAAG C^TTAAG
AflIII 4361 ACACGT A^C[GA][CT]GT
AseI 1124 ATTAAT AT^TAAT
AsnI 1124 ATTAAT AT^TAAT
Asp718I 3611 GGTACC G^GTACC
AvaI 3552 CCCGAG C^[CT]CG[GA]G
BanI 3611 GGTACC G^G[CT][GA]CC
BbrPI 4362 CACGTG CAC^GTG
BbvCI 917 GCTGAGG CCTCAGC(-5/-2)
BcgI 3730 GCACATTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2613 TGATCA T^GATCA
BfrI 4239 CTTAAG C^TTAAG
BglI 1711 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1509 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4351 TCCGGT [AT]^CCGG[AT]
BsmBI 383 GAGACG CGTCTC(1/5)
BsoBI 3552 CCCGAG C^[CT]CG[GA]G
BstAPI 3122 GCACAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 621 CGGCCA [CT]^GGCC[GA]
EarI 2425 GAAGAG CTCTTC(1/4)
EcoT22I 933 ATGCAT ATGCA^T
FauI 4344 CCCGC CCCGC(4/6)
FbaI 2613 TGATCA T^GATCA
HaeII 1465 GGCGCT [GA]GCGC^[CT]
KpnI 3611 GGTACC GGTAC^C
Ksp632I 2425 GAAGAG CTCTTC(1/4)
MamI 1509 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 262 CATATG CA^TATG
NsiI 933 ATGCAT ATGCA^T
PmaCI 4362 CACGTG CAC^GTG
PmlI 4362 CACGTG CAC^GTG
PshBI 1124 ATTAAT AT^TAAT
ScaI 2194 AGTACT AGT^ACT
SnaBI 467 TACGTA TAC^GTA
XcmI 2237 CCAGGATGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 3506 GTAGAC GT^[AC][GT]AC
3691 GTCTAC
AccII 2 CGCG CG^CG
17 CGCG
AcyI 147 GACGCC G[GA]^CG[CT]C
642 GGCGTC
AocI 1065 CCTTAGG CC^T[ACGT]AGG
1743 CCTCAGG
BciVI 464 GGATAC GTATCC(6/5)
3910 GTATCC
BcnI 1221 CCCGG CC^[GC]GG
3587 CCCGG
BlpI 2828 GCTCAGC GC^T[ACGT]AGC
3030 GCTGAGC
Bpu1102I 2828 GCTCAGC GC^T[ACGT]AGC
3030 GCTGAGC
BsaHI 147 GACGCC G[GA]^CG[CT]C
642 GGCGTC
BsaI 1683 GGTCTC GGTCTC(1/5)
3840 GGTCTC
BsmI 881 GCATTC GAATGC(1/-1)
4490 GCATTC
Bsp1407I 1031 TGTACA T^GTACA
3756 TGTACA
BsrBI 20 GAGCGG CCGCTC(-3/-3)
336 GAGCGG
BsrGI 1031 TGTACA T^GTACA
3756 TGTACA
BstUI 2 CGCG CG^CG
17 CGCG
Bsu36I 1065 CCTTAGG CC^T[ACGT]AGG
1743 CCTCAGG
CelII 2828 GCTCAGC GC^T[ACGT]AGC
3030 GCTGAGC
DraIII 696 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
3760 CACTAAGTG
DrdI 33 GACGAGCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3424 GACATAAATGTC
DsaI 713 CCATGG C^C[GA][CT]GG
2753 CCATGG
Eco81I 1065 CCTTAGG CC^T[ACGT]AGG
1743 CCTCAGG
EcoNI 3672 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4455 CCTGACCAAGG
EcoRI 1111 GAATTC G^AATTC
2159 GAATTC
Hin1I 147 GACGCC G[GA]^CG[CT]C
642 GGCGTC
HindIII 962 AAGCTT A^AGCTT
3109 AAGCTT
MfeI 574 CAATTG C^AATTG
4190 CAATTG
MunI 574 CAATTG C^AATTG
4190 CAATTG
MvnI 2 CGCG CG^CG
17 CGCG
NciI 1221 CCCGG CC^[GC]GG
3587 CCCGG
NcoI 713 CCATGG C^CATGG
2753 CCATGG
PflMI 452 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1488 CCAGCACTTGG
PvuII 81 CAGCTG CAG^CTG
249 CAGCTG
SspBI 1031 TGTACA T^GTACA
3756 TGTACA
SspI 1346 AATATT AAT^ATT
2513 AATATT
Van91I 452 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1488 CCAGCACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstBI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
NspV PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI