##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AcyI AgeI AocI
Aor51HI AscI Asp718I AviII AvrII
BbeI BbrPI BcgI BglI BlnI
BsaAI BsaHI BsiEI BsiWI BsmI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I CfoI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I FseI
FspI HaeII HhaI Hin1I HinP1I
KasI KpnI KspI MfeI MluI
MunI NarI NdeI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 107 CGCG CG^CG
AccIII 723 TCCGGA T^CCGGA
AclI 4485 AACGTT AA^CGTT
AflII 1737 CTTAAG C^TTAAG
Alw44I 1176 GTGCAC G^TGCAC
AlwNI 173 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1176 GTGCAC G^TGCAC
BamHI 446 GGATCC G^GATCC
BanI 4161 GGCACC G^G[CT][GA]CC
BbvCI 1724 GCTGAGG CCTCAGC(-5/-2)
BfrI 1737 CTTAAG C^TTAAG
BsaWI 723 TCCGGA [AT]^CCGG[AT]
BseAI 723 TCCGGA T^CCGGA
BspDI 1775 ATCGAT AT^CGAT
BspEI 723 TCCGGA T^CCGGA
BsrBI 491 GAGCGG CCGCTC(-3/-3)
BsrFI 77 GCCGGC [GA]^CCGG[CT]
BssSI 1746 CTCGTG CACGAG(-5/-1)
BstUI 107 CGCG CG^CG
BstXI 5179 CCAGGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 77 GCCGGC [GA]^CCGG[CT]
ClaI 1775 ATCGAT AT^CGAT
DrdI 729 GACCGATCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1772 GATATC GAT^ATC
EcoT22I 1607 ATGCAT ATGCA^T
FauI 390 CCCGC CCCGC(4/6)
HincII 540 GTTAAC GT[CT]^[GA]AC
HindII 540 GTTAAC GT[CT]^[GA]AC
HindIII 1278 AAGCTT A^AGCTT
HpaI 540 GTTAAC GTT^AAC
MroI 723 TCCGGA T^CCGGA
MvnI 107 CGCG CG^CG
NaeI 77 GCCGGC GCC^GGC
NgoMIV 77 GCCGGC G^CCGGC
NheI 2369 GCTAGC G^CTAGC
NsiI 1607 ATGCAT ATGCA^T
PaeR7I 4912 CTCGAG C^TCGAG
PshAI 258 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4485 AACGTT AA^CGTT
ScaI 1946 AGTACT AGT^ACT
XhoI 4912 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AhdI 39 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3274 GACTTCTTGTC
ApaI 574 GGGCCC GGGCC^C
5175 GGGCCC
Asp700I 797 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4730 GAACACATTC
AspEI 39 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3274 GACTTCTTGTC
AvaI 134 CCCGAG C^[CT]CG[GA]G
4912 CTCGAG
BciVI 864 GTATCC GTATCC(6/5)
1935 GTATCC
BclI 1129 TGATCA T^GATCA
2810 TGATCA
BlpI 3777 GCTGAGC GC^T[ACGT]AGC
4530 GCTCAGC
Bpu1102I 3777 GCTGAGC GC^T[ACGT]AGC
4530 GCTCAGC
BsaBI 889 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
984 GATAAAGATC
BsmBI 1187 CGTCTC CGTCTC(1/5)
1439 GAGACG
BsoBI 134 CCCGAG C^[CT]CG[GA]G
4912 CTCGAG
BspHI 1762 TCATGA T^CATGA
3378 TCATGA
CelII 3777 GCTGAGC GC^T[ACGT]AGC
4530 GCTCAGC
Eam1105I 39 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3274 GACTTCTTGTC
EcoRI 3025 GAATTC G^AATTC
3461 GAATTC
FbaI 1129 TGATCA T^GATCA
2810 TGATCA
HgaI 45 GCGTC GACGC(5/10)
550 GCGTC
MamI 889 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
984 GATAAAGATC
PstI 179 CTGCAG CTGCA^G
5086 CTGCAG
RcaI 1762 TCATGA T^CATGA
3378 TCATGA
SacI 4668 GAGCTC GAGCT^C
4901 GAGCTC
XbaI 2313 TCTAGA T^CTAGA
4958 TCTAGA
XmnI 797 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4730 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AspI AvaII BalI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstYI BstZ17I
Cac8I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PshBI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII