##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BciVI BclI
BlnI BsaBI BsaHI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FauI FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MslI MunI MvnI
NarI NdeI NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 1365 AACGTT AA^CGTT
AcsI 3018 AAATTT [GA]^AATT[CT]
AflII 785 CTTAAG C^TTAAG
Aor51HI 767 AGCGCT AGC^GCT
ApoI 3018 AAATTT [GA]^AATT[CT]
AseI 2585 ATTAAT AT^TAAT
AsnI 2585 ATTAAT AT^TAAT
AvaI 878 CTCGAG C^[CT]CG[GA]G
BbrPI 689 CACGTG CAC^GTG
BbvCI 539 GCTGAGG CCTCAGC(-5/-2)
BcgI 870 GCACAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 785 CTTAAG C^TTAAG
BsaAI 689 CACGTG [CT]AC^GT[GA]
BsaWI 306 TCCGGT [AT]^CCGG[AT]
BsoBI 878 CTCGAG C^[CT]CG[GA]G
BspHI 1337 TCATGA T^CATGA
BspLU11I 2113 ACATGT A^CATGT
BsrBI 806 GAGCGG CCGCTC(-3/-3)
BsrFI 141 GCCGGC [GA]^CCGG[CT]
BstEII 3079 GGTGACC G^GT[ACGT]ACC
BstPI 3079 GGTGACC G^GT[ACGT]ACC
BstXI 33 CCAGTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 141 GCCGGC [GA]^CCGG[CT]
DraIII 461 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1443 GACTTCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 767 AGCGCT AGC^GCT
EcoO65I 3079 GGTGACC G^GT[ACGT]ACC
EcoT22I 2463 ATGCAT ATGCA^T
HgaI 262 GACGC GACGC(5/10)
NaeI 141 GCCGGC GCC^GGC
NgoMIV 141 GCCGGC G^CCGGC
NsiI 2463 ATGCAT ATGCA^T
PaeR7I 878 CTCGAG C^TCGAG
PflMI 507 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 689 CACGTG CAC^GTG
PmlI 689 CACGTG CAC^GTG
PshAI 1198 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2585 ATTAAT AT^TAAT
Psp1406I 1365 AACGTT AA^CGTT
RcaI 1337 TCATGA T^CATGA
SacI 401 GAGCTC GAGCT^C
SphI 1640 GCATGC GCATG^C
Van91I 507 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 878 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 734 GTAGAC GT^[AC][GT]AC
1462 GTCTAC
AflIII 2113 ACATGT A^C[GA][CT]GT
3324 ACGTGT
AlwNI 1082 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2217 CAGTCCCTG
AocI 1022 CCTCAGG CC^T[ACGT]AGG
3120 CCTGAGG
BalI 685 TGGCCA TGG^CCA
3362 TGGCCA
BanI 2726 GGCACC G^G[CT][GA]CC
3012 GGCACC
BsmBI 1233 CGTCTC CGTCTC(1/5)
260 GAGACG
BsrDI 1290 CATTGC GCAATG(2/0)
1799 GCAATG
BssSI 317 CACGAG CACGAG(-5/-1)
374 CACGAG
Bsu36I 1022 CCTCAGG CC^T[ACGT]AGG
3120 CCTGAGG
DraII 917 AGGACCC [GA]G^G[ACGT]CC[CT]
2888 GGGTCCC
Eco81I 1022 CCTCAGG CC^T[ACGT]AGG
3120 CCTGAGG
EcoO109I 917 AGGACCC [GA]G^G[ACGT]CC[CT]
2888 GGGTCCC
MluNI 685 TGGCCA TGG^CCA
3362 TGGCCA
MscI 685 TGGCCA TGG^CCA
3362 TGGCCA
PpuMI 917 AGGACCC [GA]G^G[AT]CC[CT]
2888 GGGTCCC
PvuII 794 CAGCTG CAG^CTG
1836 CAGCTG
SapI 77 GAAGAGC GCTCTTC(1/4)
632 GAAGAGC
StuI 874 AGGCCT AGG^CCT
3057 AGGCCT
XcmI 1869 CCAAACAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2212 CCACACAGTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI