################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BbeI BciVI BclI BlnI BsaBI BsaHI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI MvnI NarI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AclI 1365 AACGTT AA^CGTT AcsI 3018 AAATTT [GA]^AATT[CT] AflII 785 CTTAAG C^TTAAG Aor51HI 767 AGCGCT AGC^GCT ApoI 3018 AAATTT [GA]^AATT[CT] AseI 2585 ATTAAT AT^TAAT AsnI 2585 ATTAAT AT^TAAT AvaI 878 CTCGAG C^[CT]CG[GA]G BbrPI 689 CACGTG CAC^GTG BbvCI 539 GCTGAGG CCTCAGC(-5/-2) BcgI 870 GCACAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 785 CTTAAG C^TTAAG BsaAI 689 CACGTG [CT]AC^GT[GA] BsaWI 306 TCCGGT [AT]^CCGG[AT] BsoBI 878 CTCGAG C^[CT]CG[GA]G BspHI 1337 TCATGA T^CATGA BspLU11I 2113 ACATGT A^CATGT BsrBI 806 GAGCGG CCGCTC(-3/-3) BsrFI 141 GCCGGC [GA]^CCGG[CT] BstEII 3079 GGTGACC G^GT[ACGT]ACC BstPI 3079 GGTGACC G^GT[ACGT]ACC BstXI 33 CCAGTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 141 GCCGGC [GA]^CCGG[CT] DraIII 461 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1443 GACTTCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 767 AGCGCT AGC^GCT EcoO65I 3079 GGTGACC G^GT[ACGT]ACC EcoT22I 2463 ATGCAT ATGCA^T HgaI 262 GACGC GACGC(5/10) NaeI 141 GCCGGC GCC^GGC NgoMIV 141 GCCGGC G^CCGGC NsiI 2463 ATGCAT ATGCA^T PaeR7I 878 CTCGAG C^TCGAG PflMI 507 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 689 CACGTG CAC^GTG PmlI 689 CACGTG CAC^GTG PshAI 1198 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2585 ATTAAT AT^TAAT Psp1406I 1365 AACGTT AA^CGTT RcaI 1337 TCATGA T^CATGA SacI 401 GAGCTC GAGCT^C SphI 1640 GCATGC GCATG^C Van91I 507 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 878 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 734 GTAGAC GT^[AC][GT]AC 1462 GTCTAC AflIII 2113 ACATGT A^C[GA][CT]GT 3324 ACGTGT AlwNI 1082 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 2217 CAGTCCCTG AocI 1022 CCTCAGG CC^T[ACGT]AGG 3120 CCTGAGG BalI 685 TGGCCA TGG^CCA 3362 TGGCCA BanI 2726 GGCACC G^G[CT][GA]CC 3012 GGCACC BsmBI 1233 CGTCTC CGTCTC(1/5) 260 GAGACG BsrDI 1290 CATTGC GCAATG(2/0) 1799 GCAATG BssSI 317 CACGAG CACGAG(-5/-1) 374 CACGAG Bsu36I 1022 CCTCAGG CC^T[ACGT]AGG 3120 CCTGAGG DraII 917 AGGACCC [GA]G^G[ACGT]CC[CT] 2888 GGGTCCC Eco81I 1022 CCTCAGG CC^T[ACGT]AGG 3120 CCTGAGG EcoO109I 917 AGGACCC [GA]G^G[ACGT]CC[CT] 2888 GGGTCCC MluNI 685 TGGCCA TGG^CCA 3362 TGGCCA MscI 685 TGGCCA TGG^CCA 3362 TGGCCA PpuMI 917 AGGACCC [GA]G^G[AT]CC[CT] 2888 GGGTCCC PvuII 794 CAGCTG CAG^CTG 1836 CAGCTG SapI 77 GAAGAGC GCTCTTC(1/4) 632 GAAGAGC StuI 874 AGGCCT AGG^CCT 3057 AGGCCT XcmI 1869 CCAAACAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2212 CCACACAGTCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AfaI AluI AlwI Asp700I AspHI AvaII BanII BbsI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI