##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BbrPI
BglI BlnI BseAI BsiEI BsiWI
BspEI BsrFI BssHII Bst1107I BstZ17I
Cfr10I CpoI DrdI EagI Eam1105I
EclXI Eco52I FseI FspI HpaI
KpnI MluI MroI NaeI NcoI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SalI SbfI
SexAI SfiI SgrAI SpeI SphI
Sse8387I SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AflIII 3664 ACATGT A^C[GA][CT]GT
AocI 114 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3073 AGCGCT AGC^GCT
AvaI 159 CCCGGG C^[CT]CG[GA]G
BamHI 2483 GGATCC G^GATCC
BbeI 33 GGCGCC GGCGC^C
BcgI 5056 CGAAGACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 4349 CTGGAG CTGGAG(16/14)
BsaAI 3921 TACGTA [CT]AC^GT[GA]
BsaBI 2019 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4936 GAGACC GGTCTC(1/5)
BsaWI 653 TCCGGT [AT]^CCGG[AT]
BsmBI 2597 GAGACG CGTCTC(1/5)
BsoBI 159 CCCGGG C^[CT]CG[GA]G
BspDI 2716 ATCGAT AT^CGAT
BspHI 730 TCATGA T^CATGA
BspLU11I 3664 ACATGT A^CATGT
BspMI 1043 GCAGGT ACCTGC(4/8)
BsrBI 91 GAGCGG CCGCTC(-3/-3)
BstBI 3295 TTCGAA TT^CGAA
BstEII 164 GGTGACC G^GT[ACGT]ACC
BstPI 164 GGTGACC G^GT[ACGT]ACC
BstXI 4229 CCACTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 114 CCTCAGG CC^T[ACGT]AGG
ClaI 2716 ATCGAT AT^CGAT
DraIII 3086 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3073 AGCGCT AGC^GCT
Eco81I 114 CCTCAGG CC^T[ACGT]AGG
EcoNI 3309 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 164 GGTGACC G^GT[ACGT]ACC
EcoRI 3289 GAATTC G^AATTC
EcoT14I 2320 CCTTGG C^C[AT][AT]GG
EcoT22I 5064 ATGCAT ATGCA^T
KasI 33 GGCGCC G^GCGCC
KspI 142 CCGCGG CCGC^GG
MamI 2019 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 33 GGCGCC GG^CGCC
NdeI 3838 CATATG CA^TATG
NheI 2477 GCTAGC G^CTAGC
NsiI 5064 ATGCAT ATGCA^T
NspV 3295 TTCGAA TT^CGAA
PflMI 848 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 3522 CAGCTG CAG^CTG
RcaI 730 TCATGA T^CATGA
SacII 142 CCGCGG CCGC^GG
SfoI 33 GGCGCC GGC^GCC
SfuI 3295 TTCGAA TT^CGAA
SmaI 159 CCCGGG CCC^GGG
SnaBI 3921 TACGTA TAC^GTA
StyI 2320 CCTTGG C^C[AT][AT]GG
Van91I 848 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2699 TCTAGA T^CTAGA
XmaCI 159 CCCGGG C^CCGGG
XmaI 159 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3156 GTCTAC GT^[AC][GT]AC
3766 GTCTAC
AclI 868 AACGTT AA^CGTT
3220 AACGTT
AcyI 33 GGCGCC G[GA]^CG[CT]C
109 GACGCC
AflII 2490 CTTAAG C^TTAAG
3201 CTTAAG
AlwNI 2160 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
5035 CAGATCCTG
AseI 2460 ATTAAT AT^TAAT
4947 ATTAAT
AsnI 2460 ATTAAT AT^TAAT
4947 ATTAAT
BbvCI 4207 CCTCAGC CCTCAGC(-5/-2)
1188 GCTGAGG
BciVI 2579 GGATAC GTATCC(6/5)
4009 GGATAC
BfrI 2490 CTTAAG C^TTAAG
3201 CTTAAG
BlpI 1392 GCTGAGC GC^T[ACGT]AGC
4145 GCTTAGC
Bpu1102I 1392 GCTGAGC GC^T[ACGT]AGC
4145 GCTTAGC
BsaHI 33 GGCGCC G[GA]^CG[CT]C
109 GACGCC
BseRI 478 GAGGAG GAGGAG(10/8)
4387 GAGGAG
BsgI 525 GTGCAG GTGCAG(16/14)
1963 GTGCAG
BsmFI 1645 GGGAC GGGAC(10/14)
1767 GGGAC
BssSI 1123 CACGAG CACGAG(-5/-1)
1960 CTCGTG
CelII 1392 GCTGAGC GC^T[ACGT]AGC
4145 GCTTAGC
DraII 1645 GGGACCC [GA]G^G[ACGT]CC[CT]
1767 GGGACCT
DsaI 142 CCGCGG C^C[GA][CT]GG
586 CCGTGG
EcoO109I 1645 GGGACCC [GA]G^G[ACGT]CC[CT]
1767 GGGACCT
EcoRV 453 GATATC GAT^ATC
2184 GATATC
HaeII 33 GGCGCC [GA]GCGC^[CT]
3073 AGCGCT
Hin1I 33 GGCGCC G[GA]^CG[CT]C
109 GACGCC
HincII 418 GTCAAC GT[CT]^[GA]AC
3373 GTTGAC
HindII 418 GTCAAC GT[CT]^[GA]AC
3373 GTTGAC
HindIII 2005 AAGCTT A^AGCTT
5138 AAGCTT
MfeI 966 CAATTG C^AATTG
1494 CAATTG
MspA1I 142 CCGCGG C[AC]G^C[GT]G
3522 CAGCTG
MunI 966 CAATTG C^AATTG
1494 CAATTG
PpuMI 1645 GGGACCC [GA]G^G[AT]CC[CT]
1767 GGGACCT
PshBI 2460 ATTAAT AT^TAAT
4947 ATTAAT
Psp1406I 868 AACGTT AA^CGTT
3220 AACGTT
PstI 42 CTGCAG CTGCA^G
329 CTGCAG
SacI 3260 GAGCTC GAGCT^C
3474 GAGCTC
SspI 879 AATATT AAT^ATT
2259 AATATT
StuI 1354 AGGCCT AGG^CCT
4315 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BalI BanI BanII BbsI BbvI
BclI BcnI BfaI BglII BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI