##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII BamHI BfrI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BsrGI BssHII Bst1107I BstAPI BstZ17I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HindIII KspI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 2969 AACGTT AA^CGTT
AflIII 3230 ACATGT A^C[GA][CT]GT
Alw44I 43 GTGCAC G^TGCAC
ApaLI 43 GTGCAC G^TGCAC
Asp700I 201 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1314 CCTAGG C^CTAGG
BalI 1637 TGGCCA TGG^CCA
BbeI 3706 GGCGCC GGCGC^C
BbrPI 1369 CACGTG CAC^GTG
BcgI 516 CGATGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2711 TGATCA T^GATCA
BglI 1156 GCCATTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1314 CCTAGG C^CTAGG
BlpI 638 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 638 GCTCAGC GC^T[ACGT]AGC
BsaBI 2712 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 946 GAGACC GGTCTC(1/5)
BsaWI 1913 TCCGGT [AT]^CCGG[AT]
BsmI 2812 GCATTC GAATGC(1/-1)
BspDI 514 ATCGAT AT^CGAT
BspLU11I 3230 ACATGT A^CATGT
BsrBI 2321 CCGCTC CCGCTC(-3/-3)
BstBI 824 TTCGAA TT^CGAA
CelII 638 GCTCAGC GC^T[ACGT]AGC
ClaI 514 ATCGAT AT^CGAT
DraI 4117 TTTAAA TTT^AAA
EcoRI 30 GAATTC G^AATTC
FbaI 2711 TGATCA T^GATCA
HpaI 2361 GTTAAC GTT^AAC
KasI 3706 GGCGCC G^GCGCC
MamI 2712 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1637 TGGCCA TGG^CCA
MscI 1637 TGGCCA TGG^CCA
NarI 3706 GGCGCC GG^CGCC
NcoI 168 CCATGG C^CATGG
NspI 3230 ACATGT [GA]CATG^[CT]
NspV 824 TTCGAA TT^CGAA
PmaCI 1369 CACGTG CAC^GTG
PmlI 1369 CACGTG CAC^GTG
Psp1406I 2969 AACGTT AA^CGTT
SfoI 3706 GGCGCC GGC^GCC
SfuI 824 TTCGAA TT^CGAA
XmnI 201 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 3961 GTCTAC GT^[AC][GT]AC
4042 GTAGAC
AcsI 30 GAATTC [GA]^AATT[CT]
4106 GAATTT
AcyI 3706 GGCGCC G[GA]^CG[CT]C
3860 GGCGTC
AocI 954 CCTGAGG CC^T[ACGT]AGG
1869 CCTCAGG
ApoI 30 GAATTC [GA]^AATT[CT]
4106 GAATTT
BbvCI 271 CCTCAGC CCTCAGC(-5/-2)
2013 GCTGAGG
BciVI 97 GGATAC GTATCC(6/5)
2845 GTATCC
BglII 588 AGATCT A^GATCT
3637 AGATCT
BsaAI 1369 CACGTG [CT]AC^GT[GA]
2306 TACGTG
BsaHI 3706 GGCGCC G[GA]^CG[CT]C
3860 GGCGTC
BspHI 1983 TCATGA T^CATGA
2414 TCATGA
BsrDI 3366 CATTGC GCAATG(2/0)
2627 GCAATG
BssSI 469 CACGAG CACGAG(-5/-1)
277 CTCGTG
Bsu36I 954 CCTGAGG CC^T[ACGT]AGG
1869 CCTCAGG
Eco81I 954 CCTGAGG CC^T[ACGT]AGG
1869 CCTCAGG
Hin1I 3706 GGCGCC G[GA]^CG[CT]C
3860 GGCGTC
HincII 2361 GTTAAC GT[CT]^[GA]AC
3814 GTCAAC
HindII 2361 GTTAAC GT[CT]^[GA]AC
3814 GTCAAC
NaeI 117 GCCGGC GCC^GGC
3913 GCCGGC
NgoMIV 117 GCCGGC G^CCGGC
3913 GCCGGC
PflMI 777 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1440 CCAAGTGCTGG
RcaI 1983 TCATGA T^CATGA
2414 TCATGA
Van91I 777 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1440 CCAAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI AlwNI ApaI Asp718I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI