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# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI Asp718I AviII BbeI BfrI
BlpI Bpu1102I BsiEI BsiWI BspDI
BspHI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI EagI
EclXI Eco52I EcoRV FseI FspI
HpaI KasI KpnI MluI NarI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RcaI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 72 TCCGGA T^CCGGA
AcyI 520 GACGCC G[GA]^CG[CT]C
AhdI 777 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 185 AGCGCT AGC^GCT
ApaI 120 GGGCCC GGGCC^C
AspEI 777 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 629 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2186 TGGCCA TGG^CCA
BcgI 447 CGAGAAACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4278 GGATAC GTATCC(6/5)
BclI 3517 TGATCA T^GATCA
BglI 3746 GCCTGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1295 AGATCT A^GATCT
BsaHI 520 GACGCC G[GA]^CG[CT]C
BseAI 72 TCCGGA T^CCGGA
BseRI 1548 GAGGAG GAGGAG(10/8)
Bsp1407I 2513 TGTACA T^GTACA
BspEI 72 TCCGGA T^CCGGA
BspLU11I 4233 ACATGT A^CATGT
BsrGI 2513 TGTACA T^GTACA
BstAPI 257 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1092 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3054 GACTATATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 777 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 185 AGCGCT AGC^GCT
EcoRI 1587 GAATTC G^AATTC
FbaI 3517 TGATCA T^GATCA
HgaI 520 GACGC GACGC(5/10)
Hin1I 520 GACGCC G[GA]^CG[CT]C
HincII 2884 GTTGAC GT[CT]^[GA]AC
HindII 2884 GTTGAC GT[CT]^[GA]AC
HindIII 849 AAGCTT A^AGCTT
KspI 175 CCGCGG CCGC^GG
MfeI 1653 CAATTG C^AATTG
MluNI 2186 TGGCCA TGG^CCA
MroI 72 TCCGGA T^CCGGA
MscI 2186 TGGCCA TGG^CCA
MunI 1653 CAATTG C^AATTG
NaeI 151 GCCGGC GCC^GGC
NgoMIV 151 GCCGGC G^CCGGC
NheI 188 GCTAGC G^CTAGC
PflFI 629 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1612 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 175 CCGCGG CCGC^GG
SapI 552 GCTCTTC GCTCTTC(1/4)
ScaI 2223 AGTACT AGT^ACT
SmlI 1450 CTTGAG C^T[CT][GA]AG
SspBI 2513 TGTACA T^GTACA
Tth111I 629 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3741 CCAGTGCCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 639 GTGCAC G^TGCAC
1634 GTGCAC
ApaLI 639 GTGCAC G^TGCAC
1634 GTGCAC
AvrII 3581 CCTAGG C^CTAGG
3832 CCTAGG
BamHI 103 GGATCC G^GATCC
240 GGATCC
BlnI 3581 CCTAGG C^CTAGG
3832 CCTAGG
BsaBI 1067 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4312 GATTCTTATC
BsaI 31 GAGACC GGTCTC(1/5)
1492 GAGACC
BsgI 1242 CTGCAC GTGCAG(16/14)
455 GTGCAG
BsmI 728 GAATGC GAATGC(1/-1)
2473 GCATTC
BspMI 1743 ACCTGC ACCTGC(4/8)
3009 GCAGGT
BsrBI 590 GAGCGG CCGCTC(-3/-3)
1453 GAGCGG
BsrFI 151 GCCGGC [GA]^CCGG[CT]
1779 GCCGGT
BstEII 1782 GGTTACC G^GT[ACGT]ACC
1917 GGTCACC
BstPI 1782 GGTTACC G^GT[ACGT]ACC
1917 GGTCACC
BstXI 1012 CCATGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1938 CCATTGTATTGG
Cfr10I 151 GCCGGC [GA]^CCGG[CT]
1779 GCCGGT
EaeI 1582 TGGCCG [CT]^GGCC[GA]
2186 TGGCCA
EcoNI 1544 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1997 CCTCGAAAAGG
EcoO65I 1782 GGTTACC G^GT[ACGT]ACC
1917 GGTCACC
EcoT22I 4210 ATGCAT ATGCA^T
4538 ATGCAT
HaeII 130 AGCGCC [GA]GCGC^[CT]
185 AGCGCT
MamI 1067 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4312 GATTCTTATC
NcoI 116 CCATGG C^CATGG
1117 CCATGG
NsiI 4210 ATGCAT ATGCA^T
4538 ATGCAT
PflMI 3741 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3840 CCATCTGATGG
PvuII 656 CAGCTG CAG^CTG
3695 CAGCTG
SmaI 99 CCCGGG CCC^GGG
235 CCCGGG
SpeI 2773 ACTAGT A^CTAGT
4585 ACTAGT
SphI 2371 GCATGC GCATG^C
4536 GCATGC
SspI 1035 AATATT AAT^ATT
4292 AATATT
Van91I 3741 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3840 CCATCTGATGG
XmaCI 99 CCCGGG C^CCGGG
235 CCCGGG
XmaI 99 CCCGGG C^CCGGG
235 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PshBI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI