##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI BamHI BbeI
BbrPI BcgI BclI BglII BsaAI
BsaBI BsiEI BsiWI BspDI BspLU11I
BsrBI BssHII BssSI Bst1107I BstAPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 545 GACGTC GACGT^C
AccII 22 CGCG CG^CG
AcyI 545 GACGTC G[GA]^CG[CT]C
AflIII 3157 ACGTGT A^C[GA][CT]GT
Aor51HI 2887 AGCGCT AGC^GCT
AviII 338 TGCGCA TGC^GCA
AvrII 4109 CCTAGG C^CTAGG
BbsI 1554 GTCTTC GAAGAC(2/6)
BciVI 422 GGATAC GTATCC(6/5)
BlnI 4109 CCTAGG C^CTAGG
BpuAI 1554 GTCTTC GAAGAC(2/6)
BsaHI 545 GACGTC G[GA]^CG[CT]C
BsmBI 115 GAGACG CGTCTC(1/5)
BsmI 1257 GAATGC GAATGC(1/-1)
BspHI 2415 TCATGA T^CATGA
BstBI 1254 TTCGAA TT^CGAA
BstEII 3253 GGTCACC G^GT[ACGT]ACC
BstPI 3253 GGTCACC G^GT[ACGT]ACC
BstUI 22 CGCG CG^CG
BstXI 4401 CCACCTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 2887 AGCGCT AGC^GCT
EcoO65I 3253 GGTCACC G^GT[ACGT]ACC
FauI 23 GCGGG CCCGC(4/6)
FspI 338 TGCGCA TGC^GCA
HaeII 2887 AGCGCT [GA]GCGC^[CT]
HgaI 580 GCGTC GACGC(5/10)
Hin1I 545 GACGTC G[GA]^CG[CT]C
HincII 542 GTTGAC GT[CT]^[GA]AC
HindII 542 GTTGAC GT[CT]^[GA]AC
HindIII 2105 AAGCTT A^AGCTT
MvnI 22 CGCG CG^CG
NspV 1254 TTCGAA TT^CGAA
PaeR7I 2832 CTCGAG C^TCGAG
RcaI 2415 TCATGA T^CATGA
SexAI 3756 ACCAGGT A^CC[AT]GGT
SfiI 445 GGCCAAAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1254 TTCGAA TT^CGAA
StuI 3277 AGGCCT AGG^CCT
XhoI 2832 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 1558 TCCGGA T^CCGGA
3917 TCCGGA
AflII 3200 CTTAAG C^TTAAG
4120 CTTAAG
AspI 412 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
576 GACTGCGTC
BfrI 3200 CTTAAG C^TTAAG
4120 CTTAAG
BglI 446 GCCAAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
695 GCCCGAATGGC
BlpI 2911 GCTCAGC GC^T[ACGT]AGC
3047 GCTGAGC
Bpu1102I 2911 GCTCAGC GC^T[ACGT]AGC
3047 GCTGAGC
BseAI 1558 TCCGGA T^CCGGA
3917 TCCGGA
BspEI 1558 TCCGGA T^CCGGA
3917 TCCGGA
BspMI 888 ACCTGC ACCTGC(4/8)
3301 GCAGGT
BsrDI 3708 CATTGC GCAATG(2/0)
334 GCAATG
BsrFI 235 ACCGGC [GA]^CCGG[CT]
922 ACCGGC
CelII 2911 GCTCAGC GC^T[ACGT]AGC
3047 GCTGAGC
Cfr10I 235 ACCGGC [GA]^CCGG[CT]
922 ACCGGC
EarI 2707 CTCTTC CTCTTC(1/4)
680 GAAGAG
EcoNI 29 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4007 CCTCGGGTAGG
Ksp632I 2707 CTCTTC CTCTTC(1/4)
680 GAAGAG
MroI 1558 TCCGGA T^CCGGA
3917 TCCGGA
NcoI 44 CCATGG C^CATGG
81 CCATGG
PflFI 412 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
576 GACTGCGTC
PshAI 160 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1547 GACGTTCGTC
SmaI 407 CCCGGG CCC^GGG
3167 CCCGGG
Tth111I 412 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
576 GACTGCGTC
XmaCI 407 CCCGGG C^CCGGG
3167 CCCGGG
XmaI 407 CCCGGG C^CCGGG
3167 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII