##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AgeI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        BfrI        BglI        
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstUI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraIII      
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRI       EcoRV       FseI        HindIII     
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI         1070  AAATTC               [GA]^AATT[CT]
AhdI         1765  GACCCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1081  GTGCAC               G^TGCAC
ApaLI        1081  GTGCAC               G^TGCAC
ApoI         1070  AAATTC               [GA]^AATT[CT]
Asp700I       822  GAATCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1765  GACCCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2337  TGCGCA               TGC^GCA
AvrII        2116  CCTAGG               C^CTAGG
BamHI        3361  GGATCC               G^GATCC
BbeI          895  GGCGCC               GGCGC^C
BbrPI        1123  CACGTG               CAC^GTG
BcgI          669  GCAAGAGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        2741  AGATCT               A^GATCT
BlnI         2116  CCTAGG               C^CTAGG
BlpI         2226  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2226  GCTGAGC              GC^T[ACGT]AGC
BsiEI         636  CGGTCG               CG[GA][CT]^CG
BssSI         859  CTCGTG               CACGAG(-5/-1)
BstAPI       3409  GCAAATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        2226  GCTGAGC              GC^T[ACGT]AGC
DraI         1933  TTTAAA               TTT^AAA
Eam1105I     1765  GACCCACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      1648  ATGCAT               ATGCA^T
FspI         2337  TGCGCA               TGC^GCA
HaeII         895  GGCGCC               [GA]GCGC^[CT]
HpaI         1957  GTTAAC               GTT^AAC
KasI          895  GGCGCC               G^GCGCC
NarI          895  GGCGCC               GG^CGCC
NsiI         1648  ATGCAT               ATGCA^T
PflMI        1592  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1123  CACGTG               CAC^GTG
PmlI         1123  CACGTG               CAC^GTG
SfoI          895  GGCGCC               GGC^GCC
SmaI         1436  CCCGGG               CCC^GGG
SphI         3138  GCATGC               GCATG^C
SspI         3242  AATATT               AAT^ATT
Van91I       1592  CCAGGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1436  CCCGGG               C^CCGGG
XmaI         1436  CCCGGG               C^CCGGG
XmnI          822  GAATCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

Acc65I         70  GGTACC               G^GTACC
             3276  GGTACC              
AcyI          483  GACGCC               G[GA]^CG[CT]C
              895  GGCGCC              
AlwNI        1238  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2025  CAGCCTCTG           
Asp718I        70  GGTACC               G^GTACC
             3276  GGTACC              
AspI         1138  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2432  GACCCTGTC           
BalI          557  TGGCCA               TGG^CCA
             1028  TGGCCA              
BbvCI        2789  CCTCAGC              CCTCAGC(-5/-2)
              865  GCTGAGG             
BciVI         730  GGATAC               GTATCC(6/5)
             1990  GTATCC              
BclI          989  TGATCA               T^GATCA
             1892  TGATCA              
BsaAI        1078  TACGTG               [CT]AC^GT[GA]
             1123  CACGTG              
BsaHI         483  GACGCC               G[GA]^CG[CT]C
              895  GGCGCC              
BsmBI         481  GAGACG               CGTCTC(1/5)
             3433  GAGACG              
BsrDI        1878  CATTGC               GCAATG(2/0)
              524  GCAATG              
BsrI          553  CCAGT                ACTGG(1/-1)
             3096  CCAGT               
BstXI        2081  CCAGGGCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3273  CCAGGTACCTGG        
DrdI         2199  GACTACATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3300  GACACAGAGGTC        
FauI           87  CCCGC                CCCGC(4/6)
             1378  CCCGC               
FbaI          989  TGATCA               T^GATCA
             1892  TGATCA              
HgaI          369  GACGC                GACGC(5/10)
              483  GACGC               
Hin1I         483  GACGCC               G[GA]^CG[CT]C
              895  GGCGCC              
HincII       1906  GTCAAC               GT[CT]^[GA]AC
             1957  GTTAAC              
HindII       1906  GTCAAC               GT[CT]^[GA]AC
             1957  GTTAAC              
KpnI           70  GGTACC               GGTAC^C
             3276  GGTACC              
MluNI         557  TGGCCA               TGG^CCA
             1028  TGGCCA              
MscI          557  TGGCCA               TGG^CCA
             1028  TGGCCA              
MspA1I       1129  CAGCTG               C[AC]G^C[GT]G
             1737  CAGCTG              
NdeI          691  CATATG               CA^TATG
             2350  CATATG              
PflFI        1138  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2432  GACCCTGTC           
PstI         1734  CTGCAG               CTGCA^G
             2644  CTGCAG              
PvuII        1129  CAGCTG               CAG^CTG
             1737  CAGCTG              
SapI         1093  GAAGAGC              GCTCTTC(1/4)
             1156  GAAGAGC             
SfcI         1734  CTGCAG               C^T[GA][CT]AG
             2644  CTGCAG              
StuI         1594  AGGCCT               AGG^CCT
             2988  AGGCCT              
Tth111I      1138  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2432  GACCCTGTC           
XcmI         3311  CCAGCAGCCTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3358  CCAGGATCCATTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaBI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspLU11I    
BspMI       BsrBI       BssKI       BstEII      BstF5I      
BstNI       BstPI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoO65I     EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII