##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AocI Aor51HI ApaI
AscI AseI AsnI BfrI BglI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV FseI HindIII
KspI MfeI MluI MroI MunI
MvnI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 1070 AAATTC [GA]^AATT[CT]
AhdI 1765 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1081 GTGCAC G^TGCAC
ApaLI 1081 GTGCAC G^TGCAC
ApoI 1070 AAATTC [GA]^AATT[CT]
Asp700I 822 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1765 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2337 TGCGCA TGC^GCA
AvrII 2116 CCTAGG C^CTAGG
BamHI 3361 GGATCC G^GATCC
BbeI 895 GGCGCC GGCGC^C
BbrPI 1123 CACGTG CAC^GTG
BcgI 669 GCAAGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2741 AGATCT A^GATCT
BlnI 2116 CCTAGG C^CTAGG
BlpI 2226 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2226 GCTGAGC GC^T[ACGT]AGC
BsiEI 636 CGGTCG CG[GA][CT]^CG
BssSI 859 CTCGTG CACGAG(-5/-1)
BstAPI 3409 GCAAATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2226 GCTGAGC GC^T[ACGT]AGC
DraI 1933 TTTAAA TTT^AAA
Eam1105I 1765 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1648 ATGCAT ATGCA^T
FspI 2337 TGCGCA TGC^GCA
HaeII 895 GGCGCC [GA]GCGC^[CT]
HpaI 1957 GTTAAC GTT^AAC
KasI 895 GGCGCC G^GCGCC
NarI 895 GGCGCC GG^CGCC
NsiI 1648 ATGCAT ATGCA^T
PflMI 1592 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1123 CACGTG CAC^GTG
PmlI 1123 CACGTG CAC^GTG
SfoI 895 GGCGCC GGC^GCC
SmaI 1436 CCCGGG CCC^GGG
SphI 3138 GCATGC GCATG^C
SspI 3242 AATATT AAT^ATT
Van91I 1592 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1436 CCCGGG C^CCGGG
XmaI 1436 CCCGGG C^CCGGG
XmnI 822 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 70 GGTACC G^GTACC
3276 GGTACC
AcyI 483 GACGCC G[GA]^CG[CT]C
895 GGCGCC
AlwNI 1238 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2025 CAGCCTCTG
Asp718I 70 GGTACC G^GTACC
3276 GGTACC
AspI 1138 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2432 GACCCTGTC
BalI 557 TGGCCA TGG^CCA
1028 TGGCCA
BbvCI 2789 CCTCAGC CCTCAGC(-5/-2)
865 GCTGAGG
BciVI 730 GGATAC GTATCC(6/5)
1990 GTATCC
BclI 989 TGATCA T^GATCA
1892 TGATCA
BsaAI 1078 TACGTG [CT]AC^GT[GA]
1123 CACGTG
BsaHI 483 GACGCC G[GA]^CG[CT]C
895 GGCGCC
BsmBI 481 GAGACG CGTCTC(1/5)
3433 GAGACG
BsrDI 1878 CATTGC GCAATG(2/0)
524 GCAATG
BsrI 553 CCAGT ACTGG(1/-1)
3096 CCAGT
BstXI 2081 CCAGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3273 CCAGGTACCTGG
DrdI 2199 GACTACATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3300 GACACAGAGGTC
FauI 87 CCCGC CCCGC(4/6)
1378 CCCGC
FbaI 989 TGATCA T^GATCA
1892 TGATCA
HgaI 369 GACGC GACGC(5/10)
483 GACGC
Hin1I 483 GACGCC G[GA]^CG[CT]C
895 GGCGCC
HincII 1906 GTCAAC GT[CT]^[GA]AC
1957 GTTAAC
HindII 1906 GTCAAC GT[CT]^[GA]AC
1957 GTTAAC
KpnI 70 GGTACC GGTAC^C
3276 GGTACC
MluNI 557 TGGCCA TGG^CCA
1028 TGGCCA
MscI 557 TGGCCA TGG^CCA
1028 TGGCCA
MspA1I 1129 CAGCTG C[AC]G^C[GT]G
1737 CAGCTG
NdeI 691 CATATG CA^TATG
2350 CATATG
PflFI 1138 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2432 GACCCTGTC
PstI 1734 CTGCAG CTGCA^G
2644 CTGCAG
PvuII 1129 CAGCTG CAG^CTG
1737 CAGCTG
SapI 1093 GAAGAGC GCTCTTC(1/4)
1156 GAAGAGC
SfcI 1734 CTGCAG C^T[GA][CT]AG
2644 CTGCAG
StuI 1594 AGGCCT AGG^CCT
2988 AGGCCT
Tth111I 1138 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2432 GACCCTGTC
XcmI 3311 CCAGCAGCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3358 CCAGGATCCATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrBI BssKI BstEII BstF5I
BstNI BstPI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII