################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AocI Aor51HI ApaI AscI AseI AsnI BfrI BglI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FseI HindIII KspI MfeI MluI MroI MunI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 1070 AAATTC [GA]^AATT[CT] AhdI 1765 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1081 GTGCAC G^TGCAC ApaLI 1081 GTGCAC G^TGCAC ApoI 1070 AAATTC [GA]^AATT[CT] Asp700I 822 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1765 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2337 TGCGCA TGC^GCA AvrII 2116 CCTAGG C^CTAGG BamHI 3361 GGATCC G^GATCC BbeI 895 GGCGCC GGCGC^C BbrPI 1123 CACGTG CAC^GTG BcgI 669 GCAAGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2741 AGATCT A^GATCT BlnI 2116 CCTAGG C^CTAGG BlpI 2226 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2226 GCTGAGC GC^T[ACGT]AGC BsiEI 636 CGGTCG CG[GA][CT]^CG BssSI 859 CTCGTG CACGAG(-5/-1) BstAPI 3409 GCAAATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 2226 GCTGAGC GC^T[ACGT]AGC DraI 1933 TTTAAA TTT^AAA Eam1105I 1765 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 1648 ATGCAT ATGCA^T FspI 2337 TGCGCA TGC^GCA HaeII 895 GGCGCC [GA]GCGC^[CT] HpaI 1957 GTTAAC GTT^AAC KasI 895 GGCGCC G^GCGCC NarI 895 GGCGCC GG^CGCC NsiI 1648 ATGCAT ATGCA^T PflMI 1592 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1123 CACGTG CAC^GTG PmlI 1123 CACGTG CAC^GTG SfoI 895 GGCGCC GGC^GCC SmaI 1436 CCCGGG CCC^GGG SphI 3138 GCATGC GCATG^C SspI 3242 AATATT AAT^ATT Van91I 1592 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1436 CCCGGG C^CCGGG XmaI 1436 CCCGGG C^CCGGG XmnI 822 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 70 GGTACC G^GTACC 3276 GGTACC AcyI 483 GACGCC G[GA]^CG[CT]C 895 GGCGCC AlwNI 1238 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 2025 CAGCCTCTG Asp718I 70 GGTACC G^GTACC 3276 GGTACC AspI 1138 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2432 GACCCTGTC BalI 557 TGGCCA TGG^CCA 1028 TGGCCA BbvCI 2789 CCTCAGC CCTCAGC(-5/-2) 865 GCTGAGG BciVI 730 GGATAC GTATCC(6/5) 1990 GTATCC BclI 989 TGATCA T^GATCA 1892 TGATCA BsaAI 1078 TACGTG [CT]AC^GT[GA] 1123 CACGTG BsaHI 483 GACGCC G[GA]^CG[CT]C 895 GGCGCC BsmBI 481 GAGACG CGTCTC(1/5) 3433 GAGACG BsrDI 1878 CATTGC GCAATG(2/0) 524 GCAATG BsrI 553 CCAGT ACTGG(1/-1) 3096 CCAGT BstXI 2081 CCAGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3273 CCAGGTACCTGG DrdI 2199 GACTACATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3300 GACACAGAGGTC FauI 87 CCCGC CCCGC(4/6) 1378 CCCGC FbaI 989 TGATCA T^GATCA 1892 TGATCA HgaI 369 GACGC GACGC(5/10) 483 GACGC Hin1I 483 GACGCC G[GA]^CG[CT]C 895 GGCGCC HincII 1906 GTCAAC GT[CT]^[GA]AC 1957 GTTAAC HindII 1906 GTCAAC GT[CT]^[GA]AC 1957 GTTAAC KpnI 70 GGTACC GGTAC^C 3276 GGTACC MluNI 557 TGGCCA TGG^CCA 1028 TGGCCA MscI 557 TGGCCA TGG^CCA 1028 TGGCCA MspA1I 1129 CAGCTG C[AC]G^C[GT]G 1737 CAGCTG NdeI 691 CATATG CA^TATG 2350 CATATG PflFI 1138 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2432 GACCCTGTC PstI 1734 CTGCAG CTGCA^G 2644 CTGCAG PvuII 1129 CAGCTG CAG^CTG 1737 CAGCTG SapI 1093 GAAGAGC GCTCTTC(1/4) 1156 GAAGAGC SfcI 1734 CTGCAG C^T[GA][CT]AG 2644 CTGCAG StuI 1594 AGGCCT AGG^CCT 2988 AGGCCT Tth111I 1138 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2432 GACCCTGTC XcmI 3311 CCAGCAGCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3358 CCAGGATCCATTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII