##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccI AflII AgeI AocI ApaI
AscI AseI AsnI AspI BamHI
BfrI BglI BsiEI BsiWI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI EagI EclXI
Eco52I Eco81I EcoNI EcoRV FseI
HpaI KspI MluI NdeI NheI
NotI NspV PacI PflFI PinAI
PmeI PshBI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 534 GACGTC GACGT^C
AccII 1734 CGCG CG^CG
AccIII 2219 TCCGGA T^CCGGA
AclI 2472 AACGTT AA^CGTT
AhdI 4768 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 864 GTGCAC G^TGCAC
Aor51HI 4311 AGCGCT AGC^GCT
ApaLI 864 GTGCAC G^TGCAC
Asp700I 4478 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4768 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1745 CCTAGG C^CTAGG
BalI 3274 TGGCCA TGG^CCA
BbeI 926 GGCGCC GGCGC^C
BbvCI 3217 GCTGAGG CCTCAGC(-5/-2)
BcgI 2674 GCACCTATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1745 CCTAGG C^CTAGG
BlpI 1584 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1584 GCTGAGC GC^T[ACGT]AGC
BsaWI 2219 TCCGGA [AT]^CCGG[AT]
BseAI 2219 TCCGGA T^CCGGA
Bsp1407I 1051 TGTACA T^GTACA
BspEI 2219 TCCGGA T^CCGGA
BsrGI 1051 TGTACA T^GTACA
BstEII 3995 GGTGACC G^GT[ACGT]ACC
BstPI 3995 GGTGACC G^GT[ACGT]ACC
BstUI 1734 CGCG CG^CG
CelII 1584 GCTGAGC GC^T[ACGT]AGC
DraI 3032 TTTAAA TTT^AAA
Eam1105I 4768 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4311 AGCGCT AGC^GCT
EcoO65I 3995 GGTGACC G^GT[ACGT]ACC
EcoRI 1760 GAATTC G^AATTC
KasI 926 GGCGCC G^GCGCC
MfeI 604 CAATTG C^AATTG
MluNI 3274 TGGCCA TGG^CCA
MroI 2219 TCCGGA T^CCGGA
MscI 3274 TGGCCA TGG^CCA
MunI 604 CAATTG C^AATTG
MvnI 1734 CGCG CG^CG
NaeI 3148 GCCGGC GCC^GGC
NarI 926 GGCGCC GG^CGCC
NgoMIV 3148 GCCGGC G^CCGGC
NruI 1733 TCGCGA TCG^CGA
PpuMI 2351 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 499 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2472 AACGTT AA^CGTT
SacI 3397 GAGCTC GAGCT^C
ScaI 2595 AGTACT AGT^ACT
SfoI 926 GGCGCC GGC^GCC
SmaI 1159 CCCGGG CCC^GGG
SspBI 1051 TGTACA T^GTACA
SspI 1402 AATATT AAT^ATT
StuI 1991 AGGCCT AGG^CCT
XbaI 2582 TCTAGA T^CTAGA
XcmI 1630 CCACCCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1159 CCCGGG C^CCGGG
XmaI 1159 CCCGGG C^CCGGG
XmnI 4478 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2067 GGTACC G^GTACC
2446 GGTACC
AcyI 534 GACGTC G[GA]^CG[CT]C
926 GGCGCC
Asp718I 2067 GGTACC G^GTACC
2446 GGTACC
AviII 2844 TGCGCA TGC^GCA
4328 TGCGCA
BglII 312 AGATCT A^GATCT
1111 AGATCT
BsaHI 534 GACGTC G[GA]^CG[CT]C
926 GGCGCC
BsaI 2556 GAGACC GGTCTC(1/5)
4207 GGTCTC
BsmI 1339 GAATGC GAATGC(1/-1)
2140 GCATTC
BsrDI 1897 GCAATG GCAATG(2/0)
3133 GCAATG
BssSI 1823 CACGAG CACGAG(-5/-1)
2508 CTCGTG
DraII 357 AGGCCCT [GA]G^G[ACGT]CC[CT]
2351 AGGTCCC
DraIII 1635 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
3476 CACTGGGTG
EaeI 251 TGGCCG [CT]^GGCC[GA]
3274 TGGCCA
EcoO109I 357 AGGCCCT [GA]G^G[ACGT]CC[CT]
2351 AGGTCCC
EcoT22I 1779 ATGCAT ATGCA^T
4748 ATGCAT
FspI 2844 TGCGCA TGC^GCA
4328 TGCGCA
HaeII 926 GGCGCC [GA]GCGC^[CT]
4311 AGCGCT
Hin1I 534 GACGTC G[GA]^CG[CT]C
926 GGCGCC
HindIII 987 AAGCTT A^AGCTT
2214 AAGCTT
KpnI 2067 GGTACC GGTAC^C
2446 GGTACC
NcoI 178 CCATGG C^CATGG
1067 CCATGG
NsiI 1779 ATGCAT ATGCA^T
4748 ATGCAT
PaeR7I 810 CTCGAG C^TCGAG
2682 CTCGAG
PflMI 701 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1634 CCACATTGTGG
PvuII 2019 CAGCTG CAG^CTG
3557 CAGCTG
SapI 764 GCTCTTC GCTCTTC(1/4)
4518 GCTCTTC
Van91I 701 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1634 CCACATTGTGG
XhoI 810 CTCGAG C^TCGAG
2682 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbrPI BbsI
BbvI BciVI BclI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DrdI DsaI
EarI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII