################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AclI AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI AspEI AspI BbrPI BclI BglI BsaAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII BstEII BstPI Bsu36I ClaI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FseI HgaI KspI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI SacII SalI SbfI SexAI SfiI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 2203 GGTACC G^GTACC AccI 3216 GTATAC GT^[AC][GT]AC AcyI 147 GGCGCC G[GA]^CG[CT]C AflII 107 CTTAAG C^TTAAG Alw44I 2777 GTGCAC G^TGCAC ApaLI 2777 GTGCAC G^TGCAC Asp700I 1550 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2203 GGTACC G^GTACC AviII 1114 TGCGCA TGC^GCA BamHI 2229 GGATCC G^GATCC BbeI 147 GGCGCC GGCGC^C BcgI 245 CGAGCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 107 CTTAAG C^TTAAG BlpI 382 GCTGAGC GC^T[ACGT]AGC Bpu1102I 382 GCTGAGC GC^T[ACGT]AGC BsaBI 3058 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 147 GGCGCC G[GA]^CG[CT]C BspHI 1579 TCATGA T^CATGA BsrBI 2602 GAGCGG CCGCTC(-3/-3) Bst1107I 3216 GTATAC GTA^TAC BstBI 1914 TTCGAA TT^CGAA BstZ17I 3216 GTATAC GTA^TAC CelII 382 GCTGAGC GC^T[ACGT]AGC CpoI 1146 CGGTCCG CG^G[AT]CCG EcoRI 3430 GAATTC G^AATTC EcoRV 1035 GATATC GAT^ATC FspI 1114 TGCGCA TGC^GCA Hin1I 147 GGCGCC G[GA]^CG[CT]C HpaI 549 GTTAAC GTT^AAC KasI 147 GGCGCC G^GCGCC KpnI 2203 GGTACC GGTAC^C MamI 3058 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 147 GGCGCC GG^CGCC NspV 1914 TTCGAA TT^CGAA PshAI 506 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 2160 CAGCTG CAG^CTG RcaI 1579 TCATGA T^CATGA RsrII 1146 CGGTCCG CG^G[AT]CCG SacI 1656 GAGCTC GAGCT^C SapI 3201 GAAGAGC GCTCTTC(1/4) ScaI 3368 AGTACT AGT^ACT SfoI 147 GGCGCC GGC^GCC SfuI 1914 TTCGAA TT^CGAA SspI 1434 AATATT AAT^ATT StuI 2873 AGGCCT AGG^CCT XmnI 1550 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 100 CGCG CG^CG 205 CGCG AccIII 7 TCCGGA T^CCGGA 413 TCCGGA AcsI 1968 AAATTC [GA]^AATT[CT] 3430 GAATTC ApoI 1968 AAATTC [GA]^AATT[CT] 3430 GAATTC BalI 320 TGGCCA TGG^CCA 1387 TGGCCA BbsI 1563 GAAGAC GAAGAC(2/6) 2770 GTCTTC BbvCI 1395 CCTCAGC CCTCAGC(-5/-2) 3244 GCTGAGG BciVI 353 GTATCC GTATCC(6/5) 1587 GTATCC BglII 817 AGATCT A^GATCT 2096 AGATCT BpuAI 1563 GAAGAC GAAGAC(2/6) 2770 GTCTTC BseAI 7 TCCGGA T^CCGGA 413 TCCGGA BsmI 1272 GAATGC GAATGC(1/-1) 3780 GAATGC BspEI 7 TCCGGA T^CCGGA 413 TCCGGA BssSI 243 CACGAG CACGAG(-5/-1) 529 CTCGTG BstUI 100 CGCG CG^CG 205 CGCG DraI 2143 TTTAAA TTT^AAA 3348 TTTAAA EaeI 320 TGGCCA [CT]^GGCC[GA] 1387 TGGCCA MluNI 320 TGGCCA TGG^CCA 1387 TGGCCA MroI 7 TCCGGA T^CCGGA 413 TCCGGA MscI 320 TGGCCA TGG^CCA 1387 TGGCCA MvnI 100 CGCG CG^CG 205 CGCG NcoI 1866 CCATGG C^CATGG 3785 CCATGG SmaI 30 CCCGGG CCC^GGG 497 CCCGGG SmlI 107 CTTAAG C^T[CT][GA]AG 3187 CTCAAG XmaCI 30 CCCGGG C^CCGGG 497 CCCGGG XmaI 30 CCCGGG C^CCGGG 497 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII AvrII BanI BanII BbvI BcnI BfaI BlnI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII