##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AspI BbrPI BclI
BglI BsaAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII BstEII BstPI Bsu36I ClaI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FbaI
FseI HgaI KspI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI SacII SalI
SbfI SexAI SfiI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2203 GGTACC G^GTACC
AccI 3216 GTATAC GT^[AC][GT]AC
AcyI 147 GGCGCC G[GA]^CG[CT]C
AflII 107 CTTAAG C^TTAAG
Alw44I 2777 GTGCAC G^TGCAC
ApaLI 2777 GTGCAC G^TGCAC
Asp700I 1550 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2203 GGTACC G^GTACC
AviII 1114 TGCGCA TGC^GCA
BamHI 2229 GGATCC G^GATCC
BbeI 147 GGCGCC GGCGC^C
BcgI 245 CGAGCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 107 CTTAAG C^TTAAG
BlpI 382 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 382 GCTGAGC GC^T[ACGT]AGC
BsaBI 3058 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 147 GGCGCC G[GA]^CG[CT]C
BspHI 1579 TCATGA T^CATGA
BsrBI 2602 GAGCGG CCGCTC(-3/-3)
Bst1107I 3216 GTATAC GTA^TAC
BstBI 1914 TTCGAA TT^CGAA
BstZ17I 3216 GTATAC GTA^TAC
CelII 382 GCTGAGC GC^T[ACGT]AGC
CpoI 1146 CGGTCCG CG^G[AT]CCG
EcoRI 3430 GAATTC G^AATTC
EcoRV 1035 GATATC GAT^ATC
FspI 1114 TGCGCA TGC^GCA
Hin1I 147 GGCGCC G[GA]^CG[CT]C
HpaI 549 GTTAAC GTT^AAC
KasI 147 GGCGCC G^GCGCC
KpnI 2203 GGTACC GGTAC^C
MamI 3058 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 147 GGCGCC GG^CGCC
NspV 1914 TTCGAA TT^CGAA
PshAI 506 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2160 CAGCTG CAG^CTG
RcaI 1579 TCATGA T^CATGA
RsrII 1146 CGGTCCG CG^G[AT]CCG
SacI 1656 GAGCTC GAGCT^C
SapI 3201 GAAGAGC GCTCTTC(1/4)
ScaI 3368 AGTACT AGT^ACT
SfoI 147 GGCGCC GGC^GCC
SfuI 1914 TTCGAA TT^CGAA
SspI 1434 AATATT AAT^ATT
StuI 2873 AGGCCT AGG^CCT
XmnI 1550 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 100 CGCG CG^CG
205 CGCG
AccIII 7 TCCGGA T^CCGGA
413 TCCGGA
AcsI 1968 AAATTC [GA]^AATT[CT]
3430 GAATTC
ApoI 1968 AAATTC [GA]^AATT[CT]
3430 GAATTC
BalI 320 TGGCCA TGG^CCA
1387 TGGCCA
BbsI 1563 GAAGAC GAAGAC(2/6)
2770 GTCTTC
BbvCI 1395 CCTCAGC CCTCAGC(-5/-2)
3244 GCTGAGG
BciVI 353 GTATCC GTATCC(6/5)
1587 GTATCC
BglII 817 AGATCT A^GATCT
2096 AGATCT
BpuAI 1563 GAAGAC GAAGAC(2/6)
2770 GTCTTC
BseAI 7 TCCGGA T^CCGGA
413 TCCGGA
BsmI 1272 GAATGC GAATGC(1/-1)
3780 GAATGC
BspEI 7 TCCGGA T^CCGGA
413 TCCGGA
BssSI 243 CACGAG CACGAG(-5/-1)
529 CTCGTG
BstUI 100 CGCG CG^CG
205 CGCG
DraI 2143 TTTAAA TTT^AAA
3348 TTTAAA
EaeI 320 TGGCCA [CT]^GGCC[GA]
1387 TGGCCA
MluNI 320 TGGCCA TGG^CCA
1387 TGGCCA
MroI 7 TCCGGA T^CCGGA
413 TCCGGA
MscI 320 TGGCCA TGG^CCA
1387 TGGCCA
MvnI 100 CGCG CG^CG
205 CGCG
NcoI 1866 CCATGG C^CATGG
3785 CCATGG
SmaI 30 CCCGGG CCC^GGG
497 CCCGGG
SmlI 107 CTTAAG C^T[CT][GA]AG
3187 CTCAAG
XmaCI 30 CCCGGG C^CCGGG
497 CCCGGG
XmaI 30 CCCGGG C^CCGGG
497 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII AvrII BanI
BanII BbvI BcnI BfaI BlnI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII