##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 632 GACGCC G[GA]^CG[CT]C
AflII 2626 CTTAAG C^TTAAG
Aor51HI 4057 AGCGCT AGC^GCT
ApaI 3535 GGGCCC GGGCC^C
AspI 2788 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2652 CTCGAG C^[CT]CG[GA]G
BalI 610 TGGCCA TGG^CCA
BbvCI 3621 CCTCAGC CCTCAGC(-5/-2)
BcnI 2662 CCGGG CC^[GC]GG
BfrI 2626 CTTAAG C^TTAAG
BglI 3169 GCCGCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3503 AGATCT A^GATCT
BlpI 1191 GCTTAGC GC^T[ACGT]AGC
BpmI 1986 CTGGAG CTGGAG(16/14)
Bpu1102I 1191 GCTTAGC GC^T[ACGT]AGC
BsaHI 632 GACGCC G[GA]^CG[CT]C
BsaI 385 GGTCTC GGTCTC(1/5)
BsoBI 2652 CTCGAG C^[CT]CG[GA]G
BstAPI 3559 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2606 CCAGCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1191 GCTTAGC GC^T[ACGT]AGC
DsaI 3426 CCATGG C^C[GA][CT]GG
Eco47III 4057 AGCGCT AGC^GCT
EcoNI 866 CCTGCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3597 GAATTC G^AATTC
HaeII 4057 AGCGCT [GA]GCGC^[CT]
Hin1I 632 GACGCC G[GA]^CG[CT]C
HindIII 3906 AAGCTT A^AGCTT
HpaI 24 GTTAAC GTT^AAC
MfeI 2995 CAATTG C^AATTG
MluNI 610 TGGCCA TGG^CCA
MscI 610 TGGCCA TGG^CCA
MunI 2995 CAATTG C^AATTG
NciI 2662 CCGGG CC^[GC]GG
NcoI 3426 CCATGG C^CATGG
NdeI 380 CATATG CA^TATG
PaeR7I 2652 CTCGAG C^TCGAG
PflFI 2788 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 3808 GTTTAAAC GTTT^AAAC
PpuMI 90 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3313 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3315 CAGCTG CAG^CTG
SacI 2433 GAGCTC GAGCT^C
SexAI 43 ACCTGGT A^CC[AT]GGT
Tth111I 2788 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2402 TCTAGA T^CTAGA
XcmI 2606 CCAGCTACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2652 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 927 CGCG CG^CG
1508 CGCG
AhdI 378 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3331 GACCACCAGTC
AlwNI 969 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
3560 CAGTGTCTG
AocI 2686 CCTTAGG CC^T[ACGT]AGG
3115 CCTTAGG
AspEI 378 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3331 GACCACCAGTC
BanI 335 GGCACC G^G[CT][GA]CC
1526 GGTGCC
BseRI 1462 CTCCTC GAGGAG(10/8)
2147 GAGGAG
BspMI 117 ACCTGC ACCTGC(4/8)
3757 ACCTGC
BsrDI 2774 CATTGC GCAATG(2/0)
660 GCAATG
BsrFI 1215 ACCGGC [GA]^CCGG[CT]
2705 ACCGGC
BstUI 927 CGCG CG^CG
1508 CGCG
Bsu36I 2686 CCTTAGG CC^T[ACGT]AGG
3115 CCTTAGG
Cfr10I 1215 ACCGGC [GA]^CCGG[CT]
2705 ACCGGC
DraII 90 GGGTCCC [GA]G^G[ACGT]CC[CT]
3535 GGGCCCC
Eam1105I 378 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3331 GACCACCAGTC
Eco81I 2686 CCTTAGG CC^T[ACGT]AGG
3115 CCTTAGG
EcoO109I 90 GGGTCCC [GA]G^G[ACGT]CC[CT]
3535 GGGCCCC
MaeII 935 ACGT A^CGT
2243 ACGT
MslI 3378 CATGTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3794 CACCACTGTG
MvnI 927 CGCG CG^CG
1508 CGCG
PstI 2000 CTGCAG CTGCA^G
2269 CTGCAG
ScaI 306 AGTACT AGT^ACT
1889 AGTACT
SphI 3404 GCATGC GCATG^C
3789 GCATGC
StuI 692 AGGCCT AGG^CCT
3710 AGGCCT
TaqI 406 TCGA T^CGA
2653 TCGA
TthHB8I 406 TCGA T^CGA
2653 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbvI BfaI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI