################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 632 GACGCC G[GA]^CG[CT]C AflII 2626 CTTAAG C^TTAAG Aor51HI 4057 AGCGCT AGC^GCT ApaI 3535 GGGCCC GGGCC^C AspI 2788 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2652 CTCGAG C^[CT]CG[GA]G BalI 610 TGGCCA TGG^CCA BbvCI 3621 CCTCAGC CCTCAGC(-5/-2) BcnI 2662 CCGGG CC^[GC]GG BfrI 2626 CTTAAG C^TTAAG BglI 3169 GCCGCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3503 AGATCT A^GATCT BlpI 1191 GCTTAGC GC^T[ACGT]AGC BpmI 1986 CTGGAG CTGGAG(16/14) Bpu1102I 1191 GCTTAGC GC^T[ACGT]AGC BsaHI 632 GACGCC G[GA]^CG[CT]C BsaI 385 GGTCTC GGTCTC(1/5) BsoBI 2652 CTCGAG C^[CT]CG[GA]G BstAPI 3559 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2606 CCAGCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1191 GCTTAGC GC^T[ACGT]AGC DsaI 3426 CCATGG C^C[GA][CT]GG Eco47III 4057 AGCGCT AGC^GCT EcoNI 866 CCTGCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3597 GAATTC G^AATTC HaeII 4057 AGCGCT [GA]GCGC^[CT] Hin1I 632 GACGCC G[GA]^CG[CT]C HindIII 3906 AAGCTT A^AGCTT HpaI 24 GTTAAC GTT^AAC MfeI 2995 CAATTG C^AATTG MluNI 610 TGGCCA TGG^CCA MscI 610 TGGCCA TGG^CCA MunI 2995 CAATTG C^AATTG NciI 2662 CCGGG CC^[GC]GG NcoI 3426 CCATGG C^CATGG NdeI 380 CATATG CA^TATG PaeR7I 2652 CTCGAG C^TCGAG PflFI 2788 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmeI 3808 GTTTAAAC GTTT^AAAC PpuMI 90 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 3313 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 3315 CAGCTG CAG^CTG SacI 2433 GAGCTC GAGCT^C SexAI 43 ACCTGGT A^CC[AT]GGT Tth111I 2788 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2402 TCTAGA T^CTAGA XcmI 2606 CCAGCTACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2652 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 927 CGCG CG^CG 1508 CGCG AhdI 378 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3331 GACCACCAGTC AlwNI 969 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 3560 CAGTGTCTG AocI 2686 CCTTAGG CC^T[ACGT]AGG 3115 CCTTAGG AspEI 378 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3331 GACCACCAGTC BanI 335 GGCACC G^G[CT][GA]CC 1526 GGTGCC BseRI 1462 CTCCTC GAGGAG(10/8) 2147 GAGGAG BspMI 117 ACCTGC ACCTGC(4/8) 3757 ACCTGC BsrDI 2774 CATTGC GCAATG(2/0) 660 GCAATG BsrFI 1215 ACCGGC [GA]^CCGG[CT] 2705 ACCGGC BstUI 927 CGCG CG^CG 1508 CGCG Bsu36I 2686 CCTTAGG CC^T[ACGT]AGG 3115 CCTTAGG Cfr10I 1215 ACCGGC [GA]^CCGG[CT] 2705 ACCGGC DraII 90 GGGTCCC [GA]G^G[ACGT]CC[CT] 3535 GGGCCCC Eam1105I 378 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3331 GACCACCAGTC Eco81I 2686 CCTTAGG CC^T[ACGT]AGG 3115 CCTTAGG EcoO109I 90 GGGTCCC [GA]G^G[ACGT]CC[CT] 3535 GGGCCCC MaeII 935 ACGT A^CGT 2243 ACGT MslI 3378 CATGTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3794 CACCACTGTG MvnI 927 CGCG CG^CG 1508 CGCG PstI 2000 CTGCAG CTGCA^G 2269 CTGCAG ScaI 306 AGTACT AGT^ACT 1889 AGTACT SphI 3404 GCATGC GCATG^C 3789 GCATGC StuI 692 AGGCCT AGG^CCT 3710 AGGCCT TaqI 406 TCGA T^CGA 2653 TCGA TthHB8I 406 TCGA T^CGA 2653 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BanII BbvI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI