##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbrPI BciVI
BfrI BglI BlnI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MluI MroI MvnI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 858 GACGTC GACGT^C
Aor51HI 105 AGCGCT AGC^GCT
BanI 624 GGCGCC G^G[CT][GA]CC
BbeI 624 GGCGCC GGCGC^C
BbvCI 571 CCTCAGC CCTCAGC(-5/-2)
BcgI 643 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3336 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3336 GCTTAGC GC^T[ACGT]AGC
BsaAI 4060 TACGTG [CT]AC^GT[GA]
BsaBI 3684 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 75 ATCGAT AT^CGAT
BsrDI 3766 CATTGC GCAATG(2/0)
BsrFI 684 ACCGGC [GA]^CCGG[CT]
BstAPI 2049 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3336 GCTTAGC GC^T[ACGT]AGC
Cfr10I 684 ACCGGC [GA]^CCGG[CT]
ClaI 75 ATCGAT AT^CGAT
DrdI 1560 GACTCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1315 CCATGG C^C[GA][CT]GG
Eco47III 105 AGCGCT AGC^GCT
FauI 42 CCCGC CCCGC(4/6)
HincII 1851 GTTGAC GT[CT]^[GA]AC
HindII 1851 GTTGAC GT[CT]^[GA]AC
KasI 624 GGCGCC G^GCGCC
MamI 3684 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 238 CCGCTG C[AC]G^C[GT]G
NarI 624 GGCGCC GG^CGCC
NcoI 1315 CCATGG C^CATGG
PaeR7I 332 CTCGAG C^TCGAG
PshAI 2624 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 500 GAGCTC GAGCT^C
SapI 1649 GAAGAGC GCTCTTC(1/4)
ScaI 4248 AGTACT AGT^ACT
SexAI 4612 ACCTGGT A^CC[AT]GGT
SfoI 624 GGCGCC GGC^GCC
SmaI 367 CCCGGG CCC^GGG
SpeI 4818 ACTAGT A^CTAGT
SphI 1207 GCATGC GCATG^C
XbaI 4271 TCTAGA T^CTAGA
XcmI 232 CCAGCACCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 332 CTCGAG C^TCGAG
XmaCI 367 CCCGGG C^CCGGG
XmaI 367 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 748 ACGTGT A^C[GA][CT]GT
4061 ACGTGT
Asp700I 3605 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4158 GAAATAATTC
BalI 292 TGGCCA TGG^CCA
2143 TGGCCA
BamHI 432 GGATCC G^GATCC
709 GGATCC
BclI 397 TGATCA T^GATCA
4285 TGATCA
BglII 2917 AGATCT A^GATCT
3865 AGATCT
BspHI 1915 TCATGA T^CATGA
4288 TCATGA
EaeI 292 TGGCCA [CT]^GGCC[GA]
2143 TGGCCA
FbaI 397 TGATCA T^GATCA
4285 TGATCA
HaeII 105 AGCGCT [GA]GCGC^[CT]
624 GGCGCC
HgaI 2467 GACGC GACGC(5/10)
1654 GCGTC
MfeI 2222 CAATTG C^AATTG
2365 CAATTG
MluNI 292 TGGCCA TGG^CCA
2143 TGGCCA
MscI 292 TGGCCA TGG^CCA
2143 TGGCCA
MunI 2222 CAATTG C^AATTG
2365 CAATTG
NdeI 2183 CATATG CA^TATG
3889 CATATG
PflMI 1310 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4795 CCAAAACTTGG
RcaI 1915 TCATGA T^CATGA
4288 TCATGA
Van91I 1310 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4795 CCAAAACTTGG
XmnI 3605 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4158 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII