################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## Acc65I AccIII AgeI AhdI AocI ApaI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BcgI BlnI BsaAI BseAI BsiWI BsmBI BspEI BsrBI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI FseI KpnI MluI MroI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SfuI SmaI SnaBI SpeI StuI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 2685 GACGTC GACGT^C AclI 675 AACGTT AA^CGTT AflIII 3068 ACATGT A^C[GA][CT]GT Alw44I 3754 GTGCAC G^TGCAC AlwI 1669 GATCC GGATC(4/5) AlwNI 715 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 54 AGCGCT AGC^GCT ApaLI 3754 GTGCAC G^TGCAC AscI 687 GGCGCGCC GG^CGCGCC AviII 2368 TGCGCA TGC^GCA BanI 180 GGCGCC G^G[CT][GA]CC BbeI 180 GGCGCC GGCGC^C BglII 1503 AGATCT A^GATCT BlpI 3296 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3296 GCTGAGC GC^T[ACGT]AGC BsaBI 1669 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 276 CGGTCG CG[GA][CT]^CG BspDI 1121 ATCGAT AT^CGAT BspLU11I 3068 ACATGT A^CATGT BsrFI 627 GCCGGT [GA]^CCGG[CT] CelII 3296 GCTGAGC GC^T[ACGT]AGC Cfr10I 627 GCCGGT [GA]^CCGG[CT] ClaI 1121 ATCGAT AT^CGAT DraIII 2338 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2181 GACAATCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 54 AGCGCT AGC^GCT EcoNI 584 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 2368 TGCGCA TGC^GCA HindIII 4294 AAGCTT A^AGCTT HpaI 4102 GTTAAC GTT^AAC KasI 180 GGCGCC G^GCGCC MamI 1669 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 798 CAATTG C^AATTG MunI 798 CAATTG C^AATTG NarI 180 GGCGCC GG^CGCC NcoI 3519 CCATGG C^CATGG NdeI 1262 CATATG CA^TATG NheI 2170 GCTAGC G^CTAGC Psp1406I 675 AACGTT AA^CGTT SalI 2190 GTCGAC G^TCGAC SbfI 3540 CCTGCAGG CCTGCA^GG SexAI 309 ACCAGGT A^CC[AT]GGT SfoI 180 GGCGCC GGC^GCC SgrAI 626 CGCCGGTG C[GA]^CCGG[CT]G Sse8387I 3540 CCTGCAGG CCTGCA^GG SspI 3206 AATATT AAT^ATT SwaI 4013 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 2190 GTCGAC GT^[AC][GT]AC 2682 GTAGAC AflII 1507 CTTAAG C^TTAAG 4180 CTTAAG AseI 2478 ATTAAT AT^TAAT 4056 ATTAAT AsnI 2478 ATTAAT AT^TAAT 4056 ATTAAT BciVI 740 GGATAC GTATCC(6/5) 2117 GTATCC BclI 1957 TGATCA T^GATCA 4301 TGATCA BfrI 1507 CTTAAG C^TTAAG 4180 CTTAAG BglI 199 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3456 GCCATCGAGGC BsaWI 612 ACCGGA [AT]^CCGG[AT] 826 ACCGGA Bsp1407I 470 TGTACA T^GTACA 3992 TGTACA BspHI 904 TCATGA T^CATGA 1342 TCATGA BspMI 1212 GCAGGT ACCTGC(4/8) 1834 GCAGGT BsrGI 470 TGTACA T^GTACA 3992 TGTACA BstYI 1503 AGATCT [GA]^GATC[CT] 1668 AGATCC EcoT14I 3519 CCATGG C^C[AT][AT]GG 3722 CCTTGG FbaI 1957 TGATCA T^GATCA 4301 TGATCA MflI 1503 AGATCT [GA]^GATC[CT] 1668 AGATCC PshBI 2478 ATTAAT AT^TAAT 4056 ATTAAT RcaI 904 TCATGA T^CATGA 1342 TCATGA SapI 1289 GAAGAGC GCTCTTC(1/4) 3039 GCTCTTC ScaI 1036 AGTACT AGT^ACT 4363 AGTACT SfiI 198 GGCCCCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3455 GGCCATCGAGGCC SmlI 1507 CTTAAG C^T[CT][GA]AG 4180 CTTAAG SphI 1294 GCATGC GCATG^C 2620 GCATGC SspBI 470 TGTACA T^GTACA 3992 TGTACA StyI 3519 CCATGG C^C[AT][AT]GG 3722 CCTTGG XhoII 1503 AGATCT [GA]^GATC[CT] 1668 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoRV EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI