##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AocI
ApaI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BcgI
BlnI BsaAI BseAI BsiWI BsmBI
BspEI BsrBI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CpoI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI FseI KpnI
MluI MroI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SfuI SmaI SnaBI
SpeI StuI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2685 GACGTC GACGT^C
AclI 675 AACGTT AA^CGTT
AflIII 3068 ACATGT A^C[GA][CT]GT
Alw44I 3754 GTGCAC G^TGCAC
AlwI 1669 GATCC GGATC(4/5)
AlwNI 715 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 54 AGCGCT AGC^GCT
ApaLI 3754 GTGCAC G^TGCAC
AscI 687 GGCGCGCC GG^CGCGCC
AviII 2368 TGCGCA TGC^GCA
BanI 180 GGCGCC G^G[CT][GA]CC
BbeI 180 GGCGCC GGCGC^C
BglII 1503 AGATCT A^GATCT
BlpI 3296 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3296 GCTGAGC GC^T[ACGT]AGC
BsaBI 1669 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 276 CGGTCG CG[GA][CT]^CG
BspDI 1121 ATCGAT AT^CGAT
BspLU11I 3068 ACATGT A^CATGT
BsrFI 627 GCCGGT [GA]^CCGG[CT]
CelII 3296 GCTGAGC GC^T[ACGT]AGC
Cfr10I 627 GCCGGT [GA]^CCGG[CT]
ClaI 1121 ATCGAT AT^CGAT
DraIII 2338 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2181 GACAATCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 54 AGCGCT AGC^GCT
EcoNI 584 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 2368 TGCGCA TGC^GCA
HindIII 4294 AAGCTT A^AGCTT
HpaI 4102 GTTAAC GTT^AAC
KasI 180 GGCGCC G^GCGCC
MamI 1669 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 798 CAATTG C^AATTG
MunI 798 CAATTG C^AATTG
NarI 180 GGCGCC GG^CGCC
NcoI 3519 CCATGG C^CATGG
NdeI 1262 CATATG CA^TATG
NheI 2170 GCTAGC G^CTAGC
Psp1406I 675 AACGTT AA^CGTT
SalI 2190 GTCGAC G^TCGAC
SbfI 3540 CCTGCAGG CCTGCA^GG
SexAI 309 ACCAGGT A^CC[AT]GGT
SfoI 180 GGCGCC GGC^GCC
SgrAI 626 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 3540 CCTGCAGG CCTGCA^GG
SspI 3206 AATATT AAT^ATT
SwaI 4013 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2190 GTCGAC GT^[AC][GT]AC
2682 GTAGAC
AflII 1507 CTTAAG C^TTAAG
4180 CTTAAG
AseI 2478 ATTAAT AT^TAAT
4056 ATTAAT
AsnI 2478 ATTAAT AT^TAAT
4056 ATTAAT
BciVI 740 GGATAC GTATCC(6/5)
2117 GTATCC
BclI 1957 TGATCA T^GATCA
4301 TGATCA
BfrI 1507 CTTAAG C^TTAAG
4180 CTTAAG
BglI 199 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3456 GCCATCGAGGC
BsaWI 612 ACCGGA [AT]^CCGG[AT]
826 ACCGGA
Bsp1407I 470 TGTACA T^GTACA
3992 TGTACA
BspHI 904 TCATGA T^CATGA
1342 TCATGA
BspMI 1212 GCAGGT ACCTGC(4/8)
1834 GCAGGT
BsrGI 470 TGTACA T^GTACA
3992 TGTACA
BstYI 1503 AGATCT [GA]^GATC[CT]
1668 AGATCC
EcoT14I 3519 CCATGG C^C[AT][AT]GG
3722 CCTTGG
FbaI 1957 TGATCA T^GATCA
4301 TGATCA
MflI 1503 AGATCT [GA]^GATC[CT]
1668 AGATCC
PshBI 2478 ATTAAT AT^TAAT
4056 ATTAAT
RcaI 904 TCATGA T^CATGA
1342 TCATGA
SapI 1289 GAAGAGC GCTCTTC(1/4)
3039 GCTCTTC
ScaI 1036 AGTACT AGT^ACT
4363 AGTACT
SfiI 198 GGCCCCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3455 GGCCATCGAGGCC
SmlI 1507 CTTAAG C^T[CT][GA]AG
4180 CTTAAG
SphI 1294 GCATGC GCATG^C
2620 GCATGC
SspBI 470 TGTACA T^GTACA
3992 TGTACA
StyI 3519 CCATGG C^C[AT][AT]GG
3722 CCTTGG
XhoII 1503 AGATCT [GA]^GATC[CT]
1668 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoRV EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI