################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Aor51HI AscI AspEI AspI AviII AvrII BbrPI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI Bsp1407I BspDI BspEI BspMI BsrFI BsrGI Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI DraIII Eam1105I Eco47III EcoRV FseI FspI KspI MamI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 2119 GGTACC G^GTACC Alw44I 2604 GTGCAC G^TGCAC AocI 4064 CCTAAGG CC^T[ACGT]AGG ApaLI 2604 GTGCAC G^TGCAC AseI 3826 ATTAAT AT^TAAT AsnI 3826 ATTAAT AT^TAAT Asp718I 2119 GGTACC G^GTACC BamHI 1457 GGATCC G^GATCC BbvCI 2800 GCTGAGG CCTCAGC(-5/-2) BciVI 2214 GTATCC GTATCC(6/5) BclI 3201 TGATCA T^GATCA BglII 491 AGATCT A^GATCT BsaWI 2225 ACCGGA [AT]^CCGG[AT] BsiWI 3162 CGTACG C^GTACG BsmI 3582 GCATTC GAATGC(1/-1) BspLU11I 3763 ACATGT A^CATGT BsrBI 90 GAGCGG CCGCTC(-3/-3) BstAPI 2144 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1758 GGTAACC G^GT[ACGT]ACC BstPI 1758 GGTAACC G^GT[ACGT]ACC Bsu36I 4064 CCTAAGG CC^T[ACGT]AGG DrdI 3456 GACCTTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4064 CCTAAGG CC^T[ACGT]AGG EcoO65I 1758 GGTAACC G^GT[ACGT]ACC EcoT22I 780 ATGCAT ATGCA^T FbaI 3201 TGATCA T^GATCA HpaI 3029 GTTAAC GTT^AAC KpnI 2119 GGTACC GGTAC^C MfeI 2965 CAATTG C^AATTG MunI 2965 CAATTG C^AATTG NsiI 780 ATGCAT ATGCA^T PmeI 3573 GTTTAAAC GTTT^AAAC PshAI 1806 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3826 ATTAAT AT^TAAT SacI 628 GAGCTC GAGCT^C SapI 625 GAAGAGC GCTCTTC(1/4) ScaI 3929 AGTACT AGT^ACT SexAI 1432 ACCTGGT A^CC[AT]GGT SphI 2144 GCATGC GCATG^C StuI 3565 AGGCCT AGG^CCT XcmI 1698 CCAGATCCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 118 GGCGCC G[GA]^CG[CT]C 1000 GGCGCC AflIII 2539 ACGTGT A^C[GA][CT]GT 3763 ACATGT AlwNI 2401 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG 3935 CAGAGCCTG ApaI 338 GGGCCC GGGCC^C 686 GGGCCC Asp700I 1356 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3702 GAATCTGTTC BalI 666 TGGCCA TGG^CCA 2501 TGGCCA BbeI 118 GGCGCC GGCGC^C 1000 GGCGCC BcgI 3462 CGAGTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1730 GCAGATTCCTCG BsaHI 118 GGCGCC G[GA]^CG[CT]C 1000 GGCGCC BsmBI 1221 CGTCTC CGTCTC(1/5) 2757 GAGACG BspHI 1320 TCATGA T^CATGA 1493 TCATGA BsrDI 282 CATTGC GCAATG(2/0) 3064 GCAATG BssHII 5 GCGCGC G^CGCGC 7 GCGCGC BssSI 2353 CTCGTG CACGAG(-5/-1) 2368 CTCGTG BstXI 657 CCAAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2330 CCATCCTGCTGG EagI 672 CGGCCG C^GGCCG 2273 CGGCCG EclXI 672 CGGCCG C^GGCCG 2273 CGGCCG Eco52I 672 CGGCCG C^GGCCG 2273 CGGCCG EcoRI 2184 GAATTC G^AATTC 3115 GAATTC HaeII 118 GGCGCC [GA]GCGC^[CT] 1000 GGCGCC Hin1I 118 GGCGCC G[GA]^CG[CT]C 1000 GGCGCC HindIII 1534 AAGCTT A^AGCTT 2472 AAGCTT KasI 118 GGCGCC G^GCGCC 1000 GGCGCC MluNI 666 TGGCCA TGG^CCA 2501 TGGCCA MscI 666 TGGCCA TGG^CCA 2501 TGGCCA NarI 118 GGCGCC GG^CGCC 1000 GGCGCC PaeR7I 2754 CTCGAG C^TCGAG 3875 CTCGAG PpuMI 110 GGGTCCC [GA]G^G[AT]CC[CT] 3454 AGGACCT PvuII 1182 CAGCTG CAG^CTG 3440 CAGCTG RcaI 1320 TCATGA T^CATGA 1493 TCATGA SfoI 118 GGCGCC GGC^GCC 1000 GGCGCC XhoI 2754 CTCGAG C^TCGAG 3875 CTCGAG XmnI 1356 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3702 GAATCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI