##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI AspEI
AspI AviII AvrII BbrPI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI Bsp1407I BspDI
BspEI BspMI BsrFI BsrGI Bst1107I
BstBI BstZ17I CelII Cfr10I ClaI
CpoI DraIII Eam1105I Eco47III EcoRV
FseI FspI KspI MamI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2119 GGTACC G^GTACC
Alw44I 2604 GTGCAC G^TGCAC
AocI 4064 CCTAAGG CC^T[ACGT]AGG
ApaLI 2604 GTGCAC G^TGCAC
AseI 3826 ATTAAT AT^TAAT
AsnI 3826 ATTAAT AT^TAAT
Asp718I 2119 GGTACC G^GTACC
BamHI 1457 GGATCC G^GATCC
BbvCI 2800 GCTGAGG CCTCAGC(-5/-2)
BciVI 2214 GTATCC GTATCC(6/5)
BclI 3201 TGATCA T^GATCA
BglII 491 AGATCT A^GATCT
BsaWI 2225 ACCGGA [AT]^CCGG[AT]
BsiWI 3162 CGTACG C^GTACG
BsmI 3582 GCATTC GAATGC(1/-1)
BspLU11I 3763 ACATGT A^CATGT
BsrBI 90 GAGCGG CCGCTC(-3/-3)
BstAPI 2144 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1758 GGTAACC G^GT[ACGT]ACC
BstPI 1758 GGTAACC G^GT[ACGT]ACC
Bsu36I 4064 CCTAAGG CC^T[ACGT]AGG
DrdI 3456 GACCTTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4064 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1758 GGTAACC G^GT[ACGT]ACC
EcoT22I 780 ATGCAT ATGCA^T
FbaI 3201 TGATCA T^GATCA
HpaI 3029 GTTAAC GTT^AAC
KpnI 2119 GGTACC GGTAC^C
MfeI 2965 CAATTG C^AATTG
MunI 2965 CAATTG C^AATTG
NsiI 780 ATGCAT ATGCA^T
PmeI 3573 GTTTAAAC GTTT^AAAC
PshAI 1806 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3826 ATTAAT AT^TAAT
SacI 628 GAGCTC GAGCT^C
SapI 625 GAAGAGC GCTCTTC(1/4)
ScaI 3929 AGTACT AGT^ACT
SexAI 1432 ACCTGGT A^CC[AT]GGT
SphI 2144 GCATGC GCATG^C
StuI 3565 AGGCCT AGG^CCT
XcmI 1698 CCAGATCCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 118 GGCGCC G[GA]^CG[CT]C
1000 GGCGCC
AflIII 2539 ACGTGT A^C[GA][CT]GT
3763 ACATGT
AlwNI 2401 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3935 CAGAGCCTG
ApaI 338 GGGCCC GGGCC^C
686 GGGCCC
Asp700I 1356 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3702 GAATCTGTTC
BalI 666 TGGCCA TGG^CCA
2501 TGGCCA
BbeI 118 GGCGCC GGCGC^C
1000 GGCGCC
BcgI 3462 CGAGTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1730 GCAGATTCCTCG
BsaHI 118 GGCGCC G[GA]^CG[CT]C
1000 GGCGCC
BsmBI 1221 CGTCTC CGTCTC(1/5)
2757 GAGACG
BspHI 1320 TCATGA T^CATGA
1493 TCATGA
BsrDI 282 CATTGC GCAATG(2/0)
3064 GCAATG
BssHII 5 GCGCGC G^CGCGC
7 GCGCGC
BssSI 2353 CTCGTG CACGAG(-5/-1)
2368 CTCGTG
BstXI 657 CCAAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2330 CCATCCTGCTGG
EagI 672 CGGCCG C^GGCCG
2273 CGGCCG
EclXI 672 CGGCCG C^GGCCG
2273 CGGCCG
Eco52I 672 CGGCCG C^GGCCG
2273 CGGCCG
EcoRI 2184 GAATTC G^AATTC
3115 GAATTC
HaeII 118 GGCGCC [GA]GCGC^[CT]
1000 GGCGCC
Hin1I 118 GGCGCC G[GA]^CG[CT]C
1000 GGCGCC
HindIII 1534 AAGCTT A^AGCTT
2472 AAGCTT
KasI 118 GGCGCC G^GCGCC
1000 GGCGCC
MluNI 666 TGGCCA TGG^CCA
2501 TGGCCA
MscI 666 TGGCCA TGG^CCA
2501 TGGCCA
NarI 118 GGCGCC GG^CGCC
1000 GGCGCC
PaeR7I 2754 CTCGAG C^TCGAG
3875 CTCGAG
PpuMI 110 GGGTCCC [GA]G^G[AT]CC[CT]
3454 AGGACCT
PvuII 1182 CAGCTG CAG^CTG
3440 CAGCTG
RcaI 1320 TCATGA T^CATGA
1493 TCATGA
SfoI 118 GGCGCC GGC^GCC
1000 GGCGCC
XhoI 2754 CTCGAG C^TCGAG
3875 CTCGAG
XmnI 1356 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3702 GAATCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI