################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI Alw44I AocI ApaLI AscI Asp718I AspEI AviII AvrII BbrPI BlnI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV FseI FspI KpnI KspI MluI MroI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI ScaI SfiI SfuI SgrAI SmaI SnaBI SphI SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflII 3454 CTTAAG C^TTAAG AlwNI 966 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 67 AGCGCT AGC^GCT AspI 2234 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 4153 TGGCCA TGG^CCA BamHI 2355 GGATCC G^GATCC BbeI 114 GGCGCC GGCGC^C BbvCI 465 CCTCAGC CCTCAGC(-5/-2) BciVI 3103 GGATAC GTATCC(6/5) BclI 2407 TGATCA T^GATCA BcnI 28 CCGGG CC^[GC]GG BfrI 3454 CTTAAG C^TTAAG BglI 3913 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3345 AGATCT A^GATCT BlpI 4944 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4944 GCTGAGC GC^T[ACGT]AGC BsaBI 938 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1777 GGTCTC GGTCTC(1/5) BsiEI 315 CGACCG CG[GA][CT]^CG BsmBI 233 GAGACG CGTCTC(1/5) BsmI 721 GAATGC GAATGC(1/-1) BsrDI 533 GCAATG GCAATG(2/0) BssHII 12 GCGCGC G^CGCGC BssSI 2759 CACGAG CACGAG(-5/-1) BstAPI 861 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 4944 GCTGAGC GC^T[ACGT]AGC Eco47III 67 AGCGCT AGC^GCT EcoT22I 988 ATGCAT ATGCA^T FbaI 2407 TGATCA T^GATCA HpaI 1280 GTTAAC GTT^AAC KasI 114 GGCGCC G^GCGCC MamI 938 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 4153 TGGCCA TGG^CCA MscI 4153 TGGCCA TGG^CCA NarI 114 GGCGCC GG^CGCC NciI 28 CCGGG CC^[GC]GG NsiI 988 ATGCAT ATGCA^T PflFI 2234 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3130 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2802 GCTCTTC GCTCTTC(1/4) SbfI 815 CCTGCAGG CCTGCA^GG SexAI 2275 ACCAGGT A^CC[AT]GGT SfoI 114 GGCGCC GGC^GCC SpeI 2909 ACTAGT A^CTAGT Sse8387I 815 CCTGCAGG CCTGCA^GG SspI 4394 AATATT AAT^ATT StuI 754 AGGCCT AGG^CCT Tth111I 2234 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 553 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 114 GGCGCC G[GA]^CG[CT]C 332 GACGCC AspHI 3748 GAGCAC G[AT]GC[AT]^C 4466 GAGCAC BanI 3 GGCACC G^G[CT][GA]CC 114 GGCGCC BcgI 126 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 694 CGATCATTCTGC BsaHI 114 GGCGCC G[GA]^CG[CT]C 332 GACGCC BsgI 4894 CTGCAC GTGCAG(16/14) 963 GTGCAG BsiHKAI 3748 GAGCAC G[AT]GC[AT]^C 4466 GAGCAC BspHI 3126 TCATGA T^CATGA 4428 TCATGA BspLU11I 959 ACATGT A^CATGT 2094 ACATGT EaeI 3391 TGGCCG [CT]^GGCC[GA] 4153 TGGCCA EcoNI 908 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2183 CCTTTGAAAGG EcoRI 2776 GAATTC G^AATTC 3122 GAATTC HaeII 67 AGCGCT [GA]GCGC^[CT] 114 GGCGCC HgaI 332 GACGC GACGC(5/10) 192 GCGTC Hin1I 114 GGCGCC G[GA]^CG[CT]C 332 GACGCC MfeI 3320 CAATTG C^AATTG 4647 CAATTG MunI 3320 CAATTG C^AATTG 4647 CAATTG NdeI 2744 CATATG CA^TATG 3845 CATATG PpuMI 345 AGGACCC [GA]G^G[AT]CC[CT] 1527 AGGTCCC RcaI 3126 TCATGA T^CATGA 4428 TCATGA XcmI 3799 CCAAGCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4335 CCAACAACCTTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApaI ApoI AseI AsnI Asp700I AvaI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI