##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I AocI ApaLI
AscI Asp718I AspEI AviII AvrII
BbrPI BlnI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRV FseI FspI KpnI
KspI MluI MroI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SphI SspBI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 3454 CTTAAG C^TTAAG
AlwNI 966 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 67 AGCGCT AGC^GCT
AspI 2234 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4153 TGGCCA TGG^CCA
BamHI 2355 GGATCC G^GATCC
BbeI 114 GGCGCC GGCGC^C
BbvCI 465 CCTCAGC CCTCAGC(-5/-2)
BciVI 3103 GGATAC GTATCC(6/5)
BclI 2407 TGATCA T^GATCA
BcnI 28 CCGGG CC^[GC]GG
BfrI 3454 CTTAAG C^TTAAG
BglI 3913 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3345 AGATCT A^GATCT
BlpI 4944 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4944 GCTGAGC GC^T[ACGT]AGC
BsaBI 938 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1777 GGTCTC GGTCTC(1/5)
BsiEI 315 CGACCG CG[GA][CT]^CG
BsmBI 233 GAGACG CGTCTC(1/5)
BsmI 721 GAATGC GAATGC(1/-1)
BsrDI 533 GCAATG GCAATG(2/0)
BssHII 12 GCGCGC G^CGCGC
BssSI 2759 CACGAG CACGAG(-5/-1)
BstAPI 861 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4944 GCTGAGC GC^T[ACGT]AGC
Eco47III 67 AGCGCT AGC^GCT
EcoT22I 988 ATGCAT ATGCA^T
FbaI 2407 TGATCA T^GATCA
HpaI 1280 GTTAAC GTT^AAC
KasI 114 GGCGCC G^GCGCC
MamI 938 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4153 TGGCCA TGG^CCA
MscI 4153 TGGCCA TGG^CCA
NarI 114 GGCGCC GG^CGCC
NciI 28 CCGGG CC^[GC]GG
NsiI 988 ATGCAT ATGCA^T
PflFI 2234 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3130 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2802 GCTCTTC GCTCTTC(1/4)
SbfI 815 CCTGCAGG CCTGCA^GG
SexAI 2275 ACCAGGT A^CC[AT]GGT
SfoI 114 GGCGCC GGC^GCC
SpeI 2909 ACTAGT A^CTAGT
Sse8387I 815 CCTGCAGG CCTGCA^GG
SspI 4394 AATATT AAT^ATT
StuI 754 AGGCCT AGG^CCT
Tth111I 2234 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 553 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 114 GGCGCC G[GA]^CG[CT]C
332 GACGCC
AspHI 3748 GAGCAC G[AT]GC[AT]^C
4466 GAGCAC
BanI 3 GGCACC G^G[CT][GA]CC
114 GGCGCC
BcgI 126 CGACAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
694 CGATCATTCTGC
BsaHI 114 GGCGCC G[GA]^CG[CT]C
332 GACGCC
BsgI 4894 CTGCAC GTGCAG(16/14)
963 GTGCAG
BsiHKAI 3748 GAGCAC G[AT]GC[AT]^C
4466 GAGCAC
BspHI 3126 TCATGA T^CATGA
4428 TCATGA
BspLU11I 959 ACATGT A^CATGT
2094 ACATGT
EaeI 3391 TGGCCG [CT]^GGCC[GA]
4153 TGGCCA
EcoNI 908 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2183 CCTTTGAAAGG
EcoRI 2776 GAATTC G^AATTC
3122 GAATTC
HaeII 67 AGCGCT [GA]GCGC^[CT]
114 GGCGCC
HgaI 332 GACGC GACGC(5/10)
192 GCGTC
Hin1I 114 GGCGCC G[GA]^CG[CT]C
332 GACGCC
MfeI 3320 CAATTG C^AATTG
4647 CAATTG
MunI 3320 CAATTG C^AATTG
4647 CAATTG
NdeI 2744 CATATG CA^TATG
3845 CATATG
PpuMI 345 AGGACCC [GA]G^G[AT]CC[CT]
1527 AGGTCCC
RcaI 3126 TCATGA T^CATGA
4428 TCATGA
XcmI 3799 CCAAGCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4335 CCAACAACCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApaI ApoI AseI
AsnI Asp700I AvaI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI