################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccIII AclI AgeI AhdI AscI AseI AsnI Asp700I AspEI AspI BamHI BciVI BclI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrGI BstBI BstEII BstPI ClaI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI MfeI MluI MroI MunI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 2244 GACGTC GACGT^C Acc65I 2014 GGTACC G^GTACC AflII 353 CTTAAG C^TTAAG AocI 3373 CCTGAGG CC^T[ACGT]AGG Aor51HI 2831 AGCGCT AGC^GCT Asp718I 2014 GGTACC G^GTACC AviII 2189 TGCGCA TGC^GCA AvrII 1365 CCTAGG C^CTAGG BalI 2150 TGGCCA TGG^CCA BcgI 604 CGAGCTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 353 CTTAAG C^TTAAG BglI 3291 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1504 AGATCT A^GATCT BlnI 1365 CCTAGG C^CTAGG BsaBI 1499 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BssHII 2480 GCGCGC G^CGCGC Bst1107I 3215 GTATAC GTA^TAC BstAPI 1001 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 3215 GTATAC GTA^TAC Bsu36I 3373 CCTGAGG CC^T[ACGT]AGG CpoI 282 CGGTCCG CG^G[AT]CCG DraI 3533 TTTAAA TTT^AAA Eco47III 2831 AGCGCT AGC^GCT Eco81I 3373 CCTGAGG CC^T[ACGT]AGG EcoNI 1374 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 2189 TGCGCA TGC^GCA HgaI 2678 GCGTC GACGC(5/10) HincII 1853 GTTGAC GT[CT]^[GA]AC HindII 1853 GTTGAC GT[CT]^[GA]AC KpnI 2014 GGTACC GGTAC^C KspI 57 CCGCGG CCGC^GG MamI 1499 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2150 TGGCCA TGG^CCA MscI 2150 TGGCCA TGG^CCA NaeI 683 GCCGGC GCC^GGC NdeI 2935 CATATG CA^TATG NgoMIV 683 GCCGGC G^CCGGC RsrII 282 CGGTCCG CG^G[AT]CCG SacII 57 CCGCGG CCGC^GG SwaI 3532 ATTTAAAT ATTT^AAAT XbaI 1576 TCTAGA T^CTAGA XcmI 3679 CCATGTGTACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 2930 GTCTAC GT^[AC][GT]AC 3215 GTATAC BbeI 277 GGCGCC GGCGC^C 410 GGCGCC BbrPI 587 CACGTG CAC^GTG 2664 CACGTG BbsI 2179 GAAGAC GAAGAC(2/6) 2241 GAAGAC BlpI 1386 GCTGAGC GC^T[ACGT]AGC 3168 GCTCAGC Bpu1102I 1386 GCTGAGC GC^T[ACGT]AGC 3168 GCTCAGC BpuAI 2179 GAAGAC GAAGAC(2/6) 2241 GAAGAC BsaAI 587 CACGTG [CT]AC^GT[GA] 2664 CACGTG BsaI 1724 GAGACC GGTCTC(1/5) 1355 GGTCTC BsaWI 1352 TCCGGT [AT]^CCGG[AT] 2464 ACCGGA BspLU11I 3311 ACATGT A^CATGT 3699 ACATGT BsrBI 179 CCGCTC CCGCTC(-3/-3) 887 GAGCGG BssSI 568 CACGAG CACGAG(-5/-1) 2158 CTCGTG BstXI 836 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2228 CCATCGACCTGG CelII 1386 GCTGAGC GC^T[ACGT]AGC 3168 GCTCAGC DrdI 2399 GACTGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2491 GACTTTGCAGTC KasI 277 GGCGCC G^GCGCC 410 GGCGCC NarI 277 GGCGCC GG^CGCC 410 GGCGCC NcoI 615 CCATGG C^CATGG 1823 CCATGG NspI 3311 ACATGT [GA]CATG^[CT] 3699 ACATGT PaeR7I 332 CTCGAG C^TCGAG 1180 CTCGAG PmaCI 587 CACGTG CAC^GTG 2664 CACGTG PmlI 587 CACGTG CAC^GTG 2664 CACGTG PstI 2074 CTGCAG CTGCA^G 2434 CTGCAG SapI 884 GAAGAGC GCTCTTC(1/4) 3196 GCTCTTC SfoI 277 GGCGCC GGC^GCC 410 GGCGCC XhoI 332 CTCGAG C^TCGAG 1180 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PshAI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI