##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AgeI AhdI AscI
AseI AsnI Asp700I AspEI AspI
BamHI BciVI BclI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrGI BstBI BstEII BstPI ClaI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2244 GACGTC GACGT^C
Acc65I 2014 GGTACC G^GTACC
AflII 353 CTTAAG C^TTAAG
AocI 3373 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2831 AGCGCT AGC^GCT
Asp718I 2014 GGTACC G^GTACC
AviII 2189 TGCGCA TGC^GCA
AvrII 1365 CCTAGG C^CTAGG
BalI 2150 TGGCCA TGG^CCA
BcgI 604 CGAGCTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 353 CTTAAG C^TTAAG
BglI 3291 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1504 AGATCT A^GATCT
BlnI 1365 CCTAGG C^CTAGG
BsaBI 1499 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssHII 2480 GCGCGC G^CGCGC
Bst1107I 3215 GTATAC GTA^TAC
BstAPI 1001 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3215 GTATAC GTA^TAC
Bsu36I 3373 CCTGAGG CC^T[ACGT]AGG
CpoI 282 CGGTCCG CG^G[AT]CCG
DraI 3533 TTTAAA TTT^AAA
Eco47III 2831 AGCGCT AGC^GCT
Eco81I 3373 CCTGAGG CC^T[ACGT]AGG
EcoNI 1374 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 2189 TGCGCA TGC^GCA
HgaI 2678 GCGTC GACGC(5/10)
HincII 1853 GTTGAC GT[CT]^[GA]AC
HindII 1853 GTTGAC GT[CT]^[GA]AC
KpnI 2014 GGTACC GGTAC^C
KspI 57 CCGCGG CCGC^GG
MamI 1499 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2150 TGGCCA TGG^CCA
MscI 2150 TGGCCA TGG^CCA
NaeI 683 GCCGGC GCC^GGC
NdeI 2935 CATATG CA^TATG
NgoMIV 683 GCCGGC G^CCGGC
RsrII 282 CGGTCCG CG^G[AT]CCG
SacII 57 CCGCGG CCGC^GG
SwaI 3532 ATTTAAAT ATTT^AAAT
XbaI 1576 TCTAGA T^CTAGA
XcmI 3679 CCATGTGTACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2930 GTCTAC GT^[AC][GT]AC
3215 GTATAC
BbeI 277 GGCGCC GGCGC^C
410 GGCGCC
BbrPI 587 CACGTG CAC^GTG
2664 CACGTG
BbsI 2179 GAAGAC GAAGAC(2/6)
2241 GAAGAC
BlpI 1386 GCTGAGC GC^T[ACGT]AGC
3168 GCTCAGC
Bpu1102I 1386 GCTGAGC GC^T[ACGT]AGC
3168 GCTCAGC
BpuAI 2179 GAAGAC GAAGAC(2/6)
2241 GAAGAC
BsaAI 587 CACGTG [CT]AC^GT[GA]
2664 CACGTG
BsaI 1724 GAGACC GGTCTC(1/5)
1355 GGTCTC
BsaWI 1352 TCCGGT [AT]^CCGG[AT]
2464 ACCGGA
BspLU11I 3311 ACATGT A^CATGT
3699 ACATGT
BsrBI 179 CCGCTC CCGCTC(-3/-3)
887 GAGCGG
BssSI 568 CACGAG CACGAG(-5/-1)
2158 CTCGTG
BstXI 836 CCAGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2228 CCATCGACCTGG
CelII 1386 GCTGAGC GC^T[ACGT]AGC
3168 GCTCAGC
DrdI 2399 GACTGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2491 GACTTTGCAGTC
KasI 277 GGCGCC G^GCGCC
410 GGCGCC
NarI 277 GGCGCC GG^CGCC
410 GGCGCC
NcoI 615 CCATGG C^CATGG
1823 CCATGG
NspI 3311 ACATGT [GA]CATG^[CT]
3699 ACATGT
PaeR7I 332 CTCGAG C^TCGAG
1180 CTCGAG
PmaCI 587 CACGTG CAC^GTG
2664 CACGTG
PmlI 587 CACGTG CAC^GTG
2664 CACGTG
PstI 2074 CTGCAG CTGCA^G
2434 CTGCAG
SapI 884 GAAGAGC GCTCTTC(1/4)
3196 GCTCTTC
SfoI 277 GGCGCC GGC^GCC
410 GGCGCC
XhoI 332 CTCGAG C^TCGAG
1180 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PshAI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI