##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AviII AvrII
BbeI BbrPI BcgI BclI BlnI
BlpI Bpu1102I BsaHI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I CelII CfoI
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 49 GGTACC G^GTACC
AccI 1163 GTAGAC GT^[AC][GT]AC
AflII 490 CTTAAG C^TTAAG
AhdI 2839 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1526 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3061 CCTAAGG CC^T[ACGT]AGG
ApaI 2067 GGGCCC GGGCC^C
Asp718I 49 GGTACC G^GTACC
AspEI 2839 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3355 GAGCTC G[AT]GC[AT]^C
AvaI 457 CCCGGG C^[CT]CG[GA]G
BanI 49 GGTACC G^G[CT][GA]CC
BciVI 1137 GGATAC GTATCC(6/5)
BfrI 490 CTTAAG C^TTAAG
BsaAI 2413 TACGTG [CT]AC^GT[GA]
BsaWI 3329 ACCGGA [AT]^CCGG[AT]
BsiHKAI 3355 GAGCTC G[AT]GC[AT]^C
BsmBI 3692 GAGACG CGTCTC(1/5)
BsoBI 457 CCCGGG C^[CT]CG[GA]G
BsrBI 3379 GAGCGG CCGCTC(-3/-3)
BsrDI 1348 GCAATG GCAATG(2/0)
BssSI 217 CTCGTG CACGAG(-5/-1)
BstAPI 272 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3061 CCTAAGG CC^T[ACGT]AGG
DrdI 1963 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2839 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3061 CCTAAGG CC^T[ACGT]AGG
HgaI 3694 GACGC GACGC(5/10)
KpnI 49 GGTACC GGTAC^C
KspI 28 CCGCGG CCGC^GG
MaeII 2414 ACGT A^CGT
MslI 1339 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 28 CCGCGG C[AC]G^C[GT]G
NheI 2026 GCTAGC G^CTAGC
NruI 3174 TCGCGA TCG^CGA
NspI 2633 ACATGC [GA]CATG^[CT]
SacI 3355 GAGCTC GAGCT^C
SacII 28 CCGCGG CCGC^GG
SapI 2512 GCTCTTC GCTCTTC(1/4)
SmaI 457 CCCGGG CCC^GGG
SspI 2657 AATATT AAT^ATT
XbaI 1119 TCTAGA T^CTAGA
XmaCI 457 CCCGGG C^CCGGG
XmaI 457 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 29 CGCG CG^CG
3175 CGCG
BamHI 955 GGATCC G^GATCC
1251 GGATCC
BbvCI 3160 CCTCAGC CCTCAGC(-5/-2)
3166 CCTCAGC
BglI 27 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3541 GCCCAGCAGGC
BglII 80 AGATCT A^GATCT
293 AGATCT
BsaBI 1609 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2707 GATACAAATC
BsaI 2291 GAGACC GGTCTC(1/5)
3085 GGTCTC
BsgI 3304 CTGCAC GTGCAG(16/14)
3154 GTGCAG
BstBI 1814 TTCGAA TT^CGAA
3701 TTCGAA
BstUI 29 CGCG CG^CG
3175 CGCG
BstXI 1635 CCAGATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1996 CCAGATTTTTGG
DraIII 629 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
1418 CACAAAGTG
EcoT22I 1926 ATGCAT ATGCA^T
2635 ATGCAT
HincII 207 GTTGAC GT[CT]^[GA]AC
874 GTTGAC
HindII 207 GTTGAC GT[CT]^[GA]AC
874 GTTGAC
MamI 1609 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2707 GATACAAATC
MvnI 29 CGCG CG^CG
3175 CGCG
NcoI 2308 CCATGG C^CATGG
2465 CCATGG
NdeI 841 CATATG CA^TATG
926 CATATG
NsiI 1926 ATGCAT ATGCA^T
2635 ATGCAT
NspV 1814 TTCGAA TT^CGAA
3701 TTCGAA
SfuI 1814 TTCGAA TT^CGAA
3701 TTCGAA
SpeI 2094 ACTAGT A^CTAGT
3520 ACTAGT
XcmI 1919 CCATCGGATGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3543 CCAGCAGGCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI