################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AgeI ApaI ApoI AscI AseI AsnI Asp718I AvrII BbrPI BclI BlnI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BssHII Bst1107I BstBI BstZ17I ClaI DrdI EcoRI EcoRV EcoT22I FbaI FseI KpnI KspI MamI MluI MroI NdeI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AflII 1969 CTTAAG C^TTAAG AhdI 3840 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2002 CCTTAGG CC^T[ACGT]AGG AspEI 3840 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 863 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 76 TGCGCA TGC^GCA BamHI 2641 GGATCC G^GATCC BbeI 1671 GGCGCC GGCGC^C BbvCI 3821 GCTGAGG CCTCAGC(-5/-2) BcgI 948 GCACGTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3882 GTATCC GTATCC(6/5) BfrI 1969 CTTAAG C^TTAAG BlpI 1147 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1147 GCTGAGC GC^T[ACGT]AGC BsiEI 89 CGGCCG CG[GA][CT]^CG Bsp1407I 1587 TGTACA T^GTACA BspLU11I 2938 ACATGT A^CATGT BsrBI 2800 GAGCGG CCGCTC(-3/-3) BsrDI 3679 GCAATG GCAATG(2/0) BsrGI 1587 TGTACA T^GTACA BstEII 2856 GGTTACC G^GT[ACGT]ACC BstPI 2856 GGTTACC G^GT[ACGT]ACC Bsu36I 2002 CCTTAGG CC^T[ACGT]AGG CelII 1147 GCTGAGC GC^T[ACGT]AGC CpoI 1743 CGGACCG CG^G[AT]CCG DraI 2781 TTTAAA TTT^AAA DraIII 2693 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 89 CGGCCG C^GGCCG Eam1105I 3840 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 89 CGGCCG C^GGCCG Eco52I 89 CGGCCG C^GGCCG Eco81I 2002 CCTTAGG CC^T[ACGT]AGG EcoO65I 2856 GGTTACC G^GT[ACGT]ACC FspI 76 TGCGCA TGC^GCA HincII 4075 GTTAAC GT[CT]^[GA]AC HindII 4075 GTTAAC GT[CT]^[GA]AC HindIII 445 AAGCTT A^AGCTT HpaI 4075 GTTAAC GTT^AAC KasI 1671 GGCGCC G^GCGCC MfeI 3640 CAATTG C^AATTG MunI 3640 CAATTG C^AATTG NarI 1671 GGCGCC GG^CGCC NheI 2299 GCTAGC G^CTAGC PaeR7I 2468 CTCGAG C^TCGAG PflFI 863 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC RsrII 1743 CGGACCG CG^G[AT]CCG SfoI 1671 GGCGCC GGC^GCC SmaI 666 CCCGGG CCC^GGG SspBI 1587 TGTACA T^GTACA Tth111I 863 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2468 CTCGAG C^TCGAG XmaCI 666 CCCGGG C^CCGGG XmaI 666 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 3827 GTAGAC GT^[AC][GT]AC 4109 GTAGAC AflIII 1257 ACGTGT A^C[GA][CT]GT 2938 ACATGT Alw44I 2314 GTGCAC G^TGCAC 3371 GTGCAC AlwNI 425 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 4399 CAGCCTCTG Aor51HI 1497 AGCGCT AGC^GCT 2877 AGCGCT ApaLI 2314 GTGCAC G^TGCAC 3371 GTGCAC Asp700I 497 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2538 GAACATTTTC BanI 1470 GGCACC G^G[CT][GA]CC 1671 GGCGCC BglII 1251 AGATCT A^GATCT 1425 AGATCT BsaAI 1256 TACGTG [CT]AC^GT[GA] 2901 CACGTA BsrFI 795 GCCGGC [GA]^CCGG[CT] 906 GCCGGC Cfr10I 795 GCCGGC [GA]^CCGG[CT] 906 GCCGGC Eco47III 1497 AGCGCT AGC^GCT 2877 AGCGCT NaeI 795 GCCGGC GCC^GGC 906 GCCGGC NgoMIV 795 GCCGGC G^CCGGC 906 GCCGGC PflMI 1038 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3953 CCAGTCCGTGG PshAI 2030 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4475 GACGCATGTC PvuII 455 CAGCTG CAG^CTG 1663 CAGCTG SacI 1348 GAGCTC GAGCT^C 2834 GAGCTC SexAI 834 ACCTGGT A^CC[AT]GGT 864 ACCTGGT StuI 71 AGGCCT AGG^CCT 1161 AGGCCT Van91I 1038 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3953 CCAGTCCGTGG XcmI 510 CCAAAAATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2609 CCATGACAACAGTGG XmnI 497 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2538 GAACATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII