##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI ApaI ApoI AscI AseI
AsnI Asp718I AvrII BbrPI BclI
BlnI BsaBI BseAI BsiWI BsmI
BspDI BspEI BspHI BssHII Bst1107I
BstBI BstZ17I ClaI DrdI EcoRI
EcoRV EcoT22I FbaI FseI KpnI
KspI MamI MluI MroI NdeI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflII 1969 CTTAAG C^TTAAG
AhdI 3840 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2002 CCTTAGG CC^T[ACGT]AGG
AspEI 3840 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 863 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 76 TGCGCA TGC^GCA
BamHI 2641 GGATCC G^GATCC
BbeI 1671 GGCGCC GGCGC^C
BbvCI 3821 GCTGAGG CCTCAGC(-5/-2)
BcgI 948 GCACGTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3882 GTATCC GTATCC(6/5)
BfrI 1969 CTTAAG C^TTAAG
BlpI 1147 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1147 GCTGAGC GC^T[ACGT]AGC
BsiEI 89 CGGCCG CG[GA][CT]^CG
Bsp1407I 1587 TGTACA T^GTACA
BspLU11I 2938 ACATGT A^CATGT
BsrBI 2800 GAGCGG CCGCTC(-3/-3)
BsrDI 3679 GCAATG GCAATG(2/0)
BsrGI 1587 TGTACA T^GTACA
BstEII 2856 GGTTACC G^GT[ACGT]ACC
BstPI 2856 GGTTACC G^GT[ACGT]ACC
Bsu36I 2002 CCTTAGG CC^T[ACGT]AGG
CelII 1147 GCTGAGC GC^T[ACGT]AGC
CpoI 1743 CGGACCG CG^G[AT]CCG
DraI 2781 TTTAAA TTT^AAA
DraIII 2693 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 89 CGGCCG C^GGCCG
Eam1105I 3840 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 89 CGGCCG C^GGCCG
Eco52I 89 CGGCCG C^GGCCG
Eco81I 2002 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2856 GGTTACC G^GT[ACGT]ACC
FspI 76 TGCGCA TGC^GCA
HincII 4075 GTTAAC GT[CT]^[GA]AC
HindII 4075 GTTAAC GT[CT]^[GA]AC
HindIII 445 AAGCTT A^AGCTT
HpaI 4075 GTTAAC GTT^AAC
KasI 1671 GGCGCC G^GCGCC
MfeI 3640 CAATTG C^AATTG
MunI 3640 CAATTG C^AATTG
NarI 1671 GGCGCC GG^CGCC
NheI 2299 GCTAGC G^CTAGC
PaeR7I 2468 CTCGAG C^TCGAG
PflFI 863 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1743 CGGACCG CG^G[AT]CCG
SfoI 1671 GGCGCC GGC^GCC
SmaI 666 CCCGGG CCC^GGG
SspBI 1587 TGTACA T^GTACA
Tth111I 863 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2468 CTCGAG C^TCGAG
XmaCI 666 CCCGGG C^CCGGG
XmaI 666 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 3827 GTAGAC GT^[AC][GT]AC
4109 GTAGAC
AflIII 1257 ACGTGT A^C[GA][CT]GT
2938 ACATGT
Alw44I 2314 GTGCAC G^TGCAC
3371 GTGCAC
AlwNI 425 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
4399 CAGCCTCTG
Aor51HI 1497 AGCGCT AGC^GCT
2877 AGCGCT
ApaLI 2314 GTGCAC G^TGCAC
3371 GTGCAC
Asp700I 497 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2538 GAACATTTTC
BanI 1470 GGCACC G^G[CT][GA]CC
1671 GGCGCC
BglII 1251 AGATCT A^GATCT
1425 AGATCT
BsaAI 1256 TACGTG [CT]AC^GT[GA]
2901 CACGTA
BsrFI 795 GCCGGC [GA]^CCGG[CT]
906 GCCGGC
Cfr10I 795 GCCGGC [GA]^CCGG[CT]
906 GCCGGC
Eco47III 1497 AGCGCT AGC^GCT
2877 AGCGCT
NaeI 795 GCCGGC GCC^GGC
906 GCCGGC
NgoMIV 795 GCCGGC G^CCGGC
906 GCCGGC
PflMI 1038 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3953 CCAGTCCGTGG
PshAI 2030 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4475 GACGCATGTC
PvuII 455 CAGCTG CAG^CTG
1663 CAGCTG
SacI 1348 GAGCTC GAGCT^C
2834 GAGCTC
SexAI 834 ACCTGGT A^CC[AT]GGT
864 ACCTGGT
StuI 71 AGGCCT AGG^CCT
1161 AGGCCT
Van91I 1038 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3953 CCAGTCCGTGG
XcmI 510 CCAAAAATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2609 CCATGACAACAGTGG
XmnI 497 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2538 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII