################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII Acc65I AclI AgeI AscI AseI AsnI Asp718I AspI BbvCI BsiWI BspHI BsrBI BsrFI BssSI BstBI Cfr10I CpoI DraI EcoT22I FseI HindIII HpaI KpnI KspI MfeI MluI MunI NaeI NcoI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 202 GTATAC GT^[AC][GT]AC AccIII 2769 TCCGGA T^CCGGA AflII 3156 CTTAAG C^TTAAG AflIII 2989 ACATGT A^C[GA][CT]GT Aor51HI 2395 AGCGCT AGC^GCT ApaI 2973 GGGCCC GGGCC^C BamHI 2564 GGATCC G^GATCC BbrPI 3712 CACGTG CAC^GTG BciVI 1773 GGATAC GTATCC(6/5) BclI 1255 TGATCA T^GATCA BfrI 3156 CTTAAG C^TTAAG BglII 226 AGATCT A^GATCT BlpI 2874 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2874 GCTCAGC GC^T[ACGT]AGC BseAI 2769 TCCGGA T^CCGGA Bsp1407I 1020 TGTACA T^GTACA BspEI 2769 TCCGGA T^CCGGA BspLU11I 2989 ACATGT A^CATGT BsrGI 1020 TGTACA T^GTACA BssHII 103 GCGCGC G^CGCGC Bst1107I 202 GTATAC GTA^TAC BstEII 658 GGTCACC G^GT[ACGT]ACC BstPI 658 GGTCACC G^GT[ACGT]ACC BstZ17I 202 GTATAC GTA^TAC CelII 2874 GCTCAGC GC^T[ACGT]AGC DraIII 1259 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 56 CGGCCG C^GGCCG EclXI 56 CGGCCG C^GGCCG Eco47III 2395 AGCGCT AGC^GCT Eco52I 56 CGGCCG C^GGCCG EcoO65I 658 GGTCACC G^GT[ACGT]ACC EcoRI 2044 GAATTC G^AATTC FbaI 1255 TGATCA T^GATCA MroI 2769 TCCGGA T^CCGGA NdeI 993 CATATG CA^TATG NheI 1515 GCTAGC G^CTAGC PaeR7I 551 CTCGAG C^TCGAG PflMI 649 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3712 CACGTG CAC^GTG PmlI 3712 CACGTG CAC^GTG SmaI 2832 CCCGGG CCC^GGG SspBI 1020 TGTACA T^GTACA StuI 907 AGGCCT AGG^CCT Van91I 649 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1696 TCTAGA T^CTAGA XhoI 551 CTCGAG C^TCGAG XmaCI 2832 CCCGGG C^CCGGG XmaI 2832 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccII 104 CGCG CG^CG 139 CGCG AfaI 1021 GTAC GT^AC 3291 GTAC AhdI 1829 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3154 GACTTAAGGTC Alw44I 3715 GTGCAC G^TGCAC 3869 GTGCAC ApaLI 3715 GTGCAC G^TGCAC 3869 GTGCAC AspEI 1829 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3154 GACTTAAGGTC AviII 531 TGCGCA TGC^GCA 1932 TGCGCA AvrII 1318 CCTAGG C^CTAGG 3810 CCTAGG BalI 1985 TGGCCA TGG^CCA 4069 TGGCCA BbeI 68 GGCGCC GGCGC^C 153 GGCGCC BcgI 473 GCAAAGATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1459 GCATGGAAATCG BglI 277 GCCAAATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2847 GCCCTGATGGC BlnI 1318 CCTAGG C^CTAGG 3810 CCTAGG BsaAI 2861 TACGTG [CT]AC^GT[GA] 3712 CACGTG BsaBI 1774 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2161 GATGATTATC BsiEI 56 CGGCCG CG[GA][CT]^CG 2259 CGACCG BsmBI 1535 GAGACG CGTCTC(1/5) 1827 GAGACG BspDI 1280 ATCGAT AT^CGAT 2168 ATCGAT BsrDI 1375 CATTGC GCAATG(2/0) 2574 GCAATG BstAPI 268 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1370 GCAGTCATTGC BstUI 104 CGCG CG^CG 139 CGCG ClaI 1280 ATCGAT AT^CGAT 2168 ATCGAT DrdI 803 GACATTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1939 GACGGAGAGGTC DsaI 3063 CCGTGG C^C[GA][CT]GG 3073 CCACGG Eam1105I 1829 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3154 GACTTAAGGTC EcoNI 899 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3214 CCTAGCAGAGG EcoRV 1861 GATATC GAT^ATC 2642 GATATC FspI 531 TGCGCA TGC^GCA 1932 TGCGCA KasI 68 GGCGCC G^GCGCC 153 GGCGCC MamI 1774 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2161 GATGATTATC MluNI 1985 TGGCCA TGG^CCA 4069 TGGCCA MscI 1985 TGGCCA TGG^CCA 4069 TGGCCA MvnI 104 CGCG CG^CG 139 CGCG NarI 68 GGCGCC GG^CGCC 153 GGCGCC PvuII 2569 CAGCTG CAG^CTG 3565 CAGCTG RsaI 1021 GTAC GT^AC 3291 GTAC SacI 2723 GAGCTC GAGCT^C 3971 GAGCTC SfoI 68 GGCGCC GGC^GCC 153 GGCGCC SphI 1311 GCATGC GCATG^C 1402 GCATGC SspI 981 AATATT AAT^ATT 1204 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AcyI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI