##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AscI        
AseI        AsnI        Asp718I     AspI        BbvCI       
BsiWI       BspHI       BsrBI       BsrFI       BssSI       
BstBI       Cfr10I      CpoI        DraI        EcoT22I     
FseI        HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MunI        NaeI        NcoI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          202  GTATAC               GT^[AC][GT]AC
AccIII       2769  TCCGGA               T^CCGGA
AflII        3156  CTTAAG               C^TTAAG
AflIII       2989  ACATGT               A^C[GA][CT]GT
Aor51HI      2395  AGCGCT               AGC^GCT
ApaI         2973  GGGCCC               GGGCC^C
BamHI        2564  GGATCC               G^GATCC
BbrPI        3712  CACGTG               CAC^GTG
BciVI        1773  GGATAC               GTATCC(6/5)
BclI         1255  TGATCA               T^GATCA
BfrI         3156  CTTAAG               C^TTAAG
BglII         226  AGATCT               A^GATCT
BlpI         2874  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2874  GCTCAGC              GC^T[ACGT]AGC
BseAI        2769  TCCGGA               T^CCGGA
Bsp1407I     1020  TGTACA               T^GTACA
BspEI        2769  TCCGGA               T^CCGGA
BspLU11I     2989  ACATGT               A^CATGT
BsrGI        1020  TGTACA               T^GTACA
BssHII        103  GCGCGC               G^CGCGC
Bst1107I      202  GTATAC               GTA^TAC
BstEII        658  GGTCACC              G^GT[ACGT]ACC
BstPI         658  GGTCACC              G^GT[ACGT]ACC
BstZ17I       202  GTATAC               GTA^TAC
CelII        2874  GCTCAGC              GC^T[ACGT]AGC
DraIII       1259  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           56  CGGCCG               C^GGCCG
EclXI          56  CGGCCG               C^GGCCG
Eco47III     2395  AGCGCT               AGC^GCT
Eco52I         56  CGGCCG               C^GGCCG
EcoO65I       658  GGTCACC              G^GT[ACGT]ACC
EcoRI        2044  GAATTC               G^AATTC
FbaI         1255  TGATCA               T^GATCA
MroI         2769  TCCGGA               T^CCGGA
NdeI          993  CATATG               CA^TATG
NheI         1515  GCTAGC               G^CTAGC
PaeR7I        551  CTCGAG               C^TCGAG
PflMI         649  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        3712  CACGTG               CAC^GTG
PmlI         3712  CACGTG               CAC^GTG
SmaI         2832  CCCGGG               CCC^GGG
SspBI        1020  TGTACA               T^GTACA
StuI          907  AGGCCT               AGG^CCT
Van91I        649  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1696  TCTAGA               T^CTAGA
XhoI          551  CTCGAG               C^TCGAG
XmaCI        2832  CCCGGG               C^CCGGG
XmaI         2832  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccII         104  CGCG                 CG^CG
              139  CGCG                
AfaI         1021  GTAC                 GT^AC
             3291  GTAC                
AhdI         1829  GACGCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3154  GACTTAAGGTC         
Alw44I       3715  GTGCAC               G^TGCAC
             3869  GTGCAC              
ApaLI        3715  GTGCAC               G^TGCAC
             3869  GTGCAC              
AspEI        1829  GACGCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3154  GACTTAAGGTC         
AviII         531  TGCGCA               TGC^GCA
             1932  TGCGCA              
AvrII        1318  CCTAGG               C^CTAGG
             3810  CCTAGG              
BalI         1985  TGGCCA               TGG^CCA
             4069  TGGCCA              
BbeI           68  GGCGCC               GGCGC^C
              153  GGCGCC              
BcgI          473  GCAAAGATTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1459  GCATGGAAATCG        
BglI          277  GCCAAATAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2847  GCCCTGATGGC         
BlnI         1318  CCTAGG               C^CTAGG
             3810  CCTAGG              
BsaAI        2861  TACGTG               [CT]AC^GT[GA]
             3712  CACGTG              
BsaBI        1774  GATACTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2161  GATGATTATC          
BsiEI          56  CGGCCG               CG[GA][CT]^CG
             2259  CGACCG              
BsmBI        1535  GAGACG               CGTCTC(1/5)
             1827  GAGACG              
BspDI        1280  ATCGAT               AT^CGAT
             2168  ATCGAT              
BsrDI        1375  CATTGC               GCAATG(2/0)
             2574  GCAATG              
BstAPI        268  GCATGACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1370  GCAGTCATTGC         
BstUI         104  CGCG                 CG^CG
              139  CGCG                
ClaI         1280  ATCGAT               AT^CGAT
             2168  ATCGAT              
DrdI          803  GACATTGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1939  GACGGAGAGGTC        
DsaI         3063  CCGTGG               C^C[GA][CT]GG
             3073  CCACGG              
Eam1105I     1829  GACGCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3154  GACTTAAGGTC         
EcoNI         899  CCTGCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3214  CCTAGCAGAGG         
EcoRV        1861  GATATC               GAT^ATC
             2642  GATATC              
FspI          531  TGCGCA               TGC^GCA
             1932  TGCGCA              
KasI           68  GGCGCC               G^GCGCC
              153  GGCGCC              
MamI         1774  GATACTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2161  GATGATTATC          
MluNI        1985  TGGCCA               TGG^CCA
             4069  TGGCCA              
MscI         1985  TGGCCA               TGG^CCA
             4069  TGGCCA              
MvnI          104  CGCG                 CG^CG
              139  CGCG                
NarI           68  GGCGCC               GG^CGCC
              153  GGCGCC              
PvuII        2569  CAGCTG               CAG^CTG
             3565  CAGCTG              
RsaI         1021  GTAC                 GT^AC
             3291  GTAC                
SacI         2723  GAGCTC               GAGCT^C
             3971  GAGCTC              
SfoI           68  GGCGCC               GGC^GCC
              153  GGCGCC              
SphI         1311  GCATGC               GCATG^C
             1402  GCATGC              
SspI          981  AATATT               AAT^ATT
             1204  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AcyI        AluI        AlwI        
AlwNI       AocI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmnI