##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AspI BbvCI
BsiWI BspHI BsrBI BsrFI BssSI
BstBI Cfr10I CpoI DraI EcoT22I
FseI HindIII HpaI KpnI KspI
MfeI MluI MunI NaeI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 202 GTATAC GT^[AC][GT]AC
AccIII 2769 TCCGGA T^CCGGA
AflII 3156 CTTAAG C^TTAAG
AflIII 2989 ACATGT A^C[GA][CT]GT
Aor51HI 2395 AGCGCT AGC^GCT
ApaI 2973 GGGCCC GGGCC^C
BamHI 2564 GGATCC G^GATCC
BbrPI 3712 CACGTG CAC^GTG
BciVI 1773 GGATAC GTATCC(6/5)
BclI 1255 TGATCA T^GATCA
BfrI 3156 CTTAAG C^TTAAG
BglII 226 AGATCT A^GATCT
BlpI 2874 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2874 GCTCAGC GC^T[ACGT]AGC
BseAI 2769 TCCGGA T^CCGGA
Bsp1407I 1020 TGTACA T^GTACA
BspEI 2769 TCCGGA T^CCGGA
BspLU11I 2989 ACATGT A^CATGT
BsrGI 1020 TGTACA T^GTACA
BssHII 103 GCGCGC G^CGCGC
Bst1107I 202 GTATAC GTA^TAC
BstEII 658 GGTCACC G^GT[ACGT]ACC
BstPI 658 GGTCACC G^GT[ACGT]ACC
BstZ17I 202 GTATAC GTA^TAC
CelII 2874 GCTCAGC GC^T[ACGT]AGC
DraIII 1259 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 56 CGGCCG C^GGCCG
EclXI 56 CGGCCG C^GGCCG
Eco47III 2395 AGCGCT AGC^GCT
Eco52I 56 CGGCCG C^GGCCG
EcoO65I 658 GGTCACC G^GT[ACGT]ACC
EcoRI 2044 GAATTC G^AATTC
FbaI 1255 TGATCA T^GATCA
MroI 2769 TCCGGA T^CCGGA
NdeI 993 CATATG CA^TATG
NheI 1515 GCTAGC G^CTAGC
PaeR7I 551 CTCGAG C^TCGAG
PflMI 649 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3712 CACGTG CAC^GTG
PmlI 3712 CACGTG CAC^GTG
SmaI 2832 CCCGGG CCC^GGG
SspBI 1020 TGTACA T^GTACA
StuI 907 AGGCCT AGG^CCT
Van91I 649 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1696 TCTAGA T^CTAGA
XhoI 551 CTCGAG C^TCGAG
XmaCI 2832 CCCGGG C^CCGGG
XmaI 2832 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 104 CGCG CG^CG
139 CGCG
AfaI 1021 GTAC GT^AC
3291 GTAC
AhdI 1829 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3154 GACTTAAGGTC
Alw44I 3715 GTGCAC G^TGCAC
3869 GTGCAC
ApaLI 3715 GTGCAC G^TGCAC
3869 GTGCAC
AspEI 1829 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3154 GACTTAAGGTC
AviII 531 TGCGCA TGC^GCA
1932 TGCGCA
AvrII 1318 CCTAGG C^CTAGG
3810 CCTAGG
BalI 1985 TGGCCA TGG^CCA
4069 TGGCCA
BbeI 68 GGCGCC GGCGC^C
153 GGCGCC
BcgI 473 GCAAAGATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1459 GCATGGAAATCG
BglI 277 GCCAAATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2847 GCCCTGATGGC
BlnI 1318 CCTAGG C^CTAGG
3810 CCTAGG
BsaAI 2861 TACGTG [CT]AC^GT[GA]
3712 CACGTG
BsaBI 1774 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2161 GATGATTATC
BsiEI 56 CGGCCG CG[GA][CT]^CG
2259 CGACCG
BsmBI 1535 GAGACG CGTCTC(1/5)
1827 GAGACG
BspDI 1280 ATCGAT AT^CGAT
2168 ATCGAT
BsrDI 1375 CATTGC GCAATG(2/0)
2574 GCAATG
BstAPI 268 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1370 GCAGTCATTGC
BstUI 104 CGCG CG^CG
139 CGCG
ClaI 1280 ATCGAT AT^CGAT
2168 ATCGAT
DrdI 803 GACATTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1939 GACGGAGAGGTC
DsaI 3063 CCGTGG C^C[GA][CT]GG
3073 CCACGG
Eam1105I 1829 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3154 GACTTAAGGTC
EcoNI 899 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3214 CCTAGCAGAGG
EcoRV 1861 GATATC GAT^ATC
2642 GATATC
FspI 531 TGCGCA TGC^GCA
1932 TGCGCA
KasI 68 GGCGCC G^GCGCC
153 GGCGCC
MamI 1774 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2161 GATGATTATC
MluNI 1985 TGGCCA TGG^CCA
4069 TGGCCA
MscI 1985 TGGCCA TGG^CCA
4069 TGGCCA
MvnI 104 CGCG CG^CG
139 CGCG
NarI 68 GGCGCC GG^CGCC
153 GGCGCC
PvuII 2569 CAGCTG CAG^CTG
3565 CAGCTG
RsaI 1021 GTAC GT^AC
3291 GTAC
SacI 2723 GAGCTC GAGCT^C
3971 GAGCTC
SfoI 68 GGCGCC GGC^GCC
153 GGCGCC
SphI 1311 GCATGC GCATG^C
1402 GCATGC
SspI 981 AATATT AAT^ATT
1204 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI