##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AccIII AclI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvrII BbeI BbrPI BcgI
BclI BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRV FbaI
FseI HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1323 GACGTC GACGT^C
AccII 488 CGCG CG^CG
AflII 2234 CTTAAG C^TTAAG
AgeI 1023 ACCGGT A^CCGGT
ApaI 1213 GGGCCC GGGCC^C
Asp700I 1304 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 30 CCCGGG C^[CT]CG[GA]G
AviII 571 TGCGCA TGC^GCA
BanI 1467 GGTGCC G^G[CT][GA]CC
BbvCI 1092 CCTCAGC CCTCAGC(-5/-2)
BciVI 1449 GGATAC GTATCC(6/5)
BfrI 2234 CTTAAG C^TTAAG
BglI 629 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 259 AGATCT A^GATCT
BsaWI 1023 ACCGGT [AT]^CCGG[AT]
BsiEI 464 CGACCG CG[GA][CT]^CG
BsmI 1681 GCATTC GAATGC(1/-1)
BsoBI 30 CCCGGG C^[CT]CG[GA]G
BspHI 499 TCATGA T^CATGA
BspMI 281 GCAGGT ACCTGC(4/8)
BstUI 488 CGCG CG^CG
EcoRI 667 GAATTC G^AATTC
EcoT14I 1176 CCATGG C^C[AT][AT]GG
FspI 571 TGCGCA TGC^GCA
HgaI 606 GACGC GACGC(5/10)
HincII 2700 GTCAAC GT[CT]^[GA]AC
HindII 2700 GTCAAC GT[CT]^[GA]AC
MvnI 488 CGCG CG^CG
NcoI 1176 CCATGG C^CATGG
PinAI 1023 ACCGGT A^CCGGT
RcaI 499 TCATGA T^CATGA
SmaI 30 CCCGGG CCC^GGG
SpeI 2338 ACTAGT A^CTAGT
StyI 1176 CCATGG C^C[AT][AT]GG
XcmI 334 CCAACAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
XmnI 1304 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 606 GACGCC G[GA]^CG[CT]C
1323 GACGTC
AocI 219 CCTCAGG CC^T[ACGT]AGG
2581 CCTCAGG
BamHI 931 GGATCC G^GATCC
3277 GGATCC
BanII 27 GAGCCC G[GA]GC[CT]^C
1213 GGGCCC
BsaHI 606 GACGCC G[GA]^CG[CT]C
1323 GACGTC
BspLU11I 2717 ACATGT A^CATGT
3041 ACATGT
BsrBI 2853 CCGCTC CCGCTC(-3/-3)
546 GAGCGG
BsrDI 2662 CATTGC GCAATG(2/0)
2863 CATTGC
BssSI 3173 CACGAG CACGAG(-5/-1)
3388 CACGAG
BstAPI 1104 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2657 GCATCCATTGC
BstXI 322 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
733 CCAGCAATCTGG
Bsu36I 219 CCTCAGG CC^T[ACGT]AGG
2581 CCTCAGG
Eco81I 219 CCTCAGG CC^T[ACGT]AGG
2581 CCTCAGG
EcoT22I 973 ATGCAT ATGCA^T
1679 ATGCAT
HaeII 505 AGCGCC [GA]GCGC^[CT]
1072 GGCGCT
Hin1I 606 GACGCC G[GA]^CG[CT]C
1323 GACGTC
HindIII 3166 AAGCTT A^AGCTT
3679 AAGCTT
MfeI 1800 CAATTG C^AATTG
2214 CAATTG
MunI 1800 CAATTG C^AATTG
2214 CAATTG
NsiI 973 ATGCAT ATGCA^T
1679 ATGCAT
PflMI 554 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2016 CCACCAAATGG
PstI 99 CTGCAG CTGCA^G
644 CTGCAG
SapI 958 GAAGAGC GCTCTTC(1/4)
2288 GCTCTTC
SexAI 343 ACCTGGT A^CC[AT]GGT
2668 ACCAGGT
SphI 478 GCATGC GCATG^C
975 GCATGC
StuI 1752 AGGCCT AGG^CCT
3153 AGGCCT
Van91I 554 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2016 CCACCAAATGG
XbaI 2826 TCTAGA T^CTAGA
3612 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AspI AvaII BalI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrFI BsrGI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoO65I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII