##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AocI ApaI AscI Asp700I AspI
AviII BamHI BbeI BbrPI BcgI
BciVI BcnI BglI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I CpoI DraIII DrdI
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HgaI Hin1I HindIII KasI KspI
MamI MluI MroI NaeI NarI
NciI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1706 GGTACC G^GTACC
AccII 49 CGCG CG^CG
AhdI 4154 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 424 GTGCAC G^TGCAC
Aor51HI 497 AGCGCT AGC^GCT
ApaLI 424 GTGCAC G^TGCAC
Asp718I 1706 GGTACC G^GTACC
AspEI 4154 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4568 CCTAGG C^CTAGG
BanI 1706 GGTACC G^G[CT][GA]CC
BbvCI 1357 CCTCAGC CCTCAGC(-5/-2)
BclI 3043 TGATCA T^GATCA
BlnI 4568 CCTAGG C^CTAGG
BsaAI 4316 TACGTG [CT]AC^GT[GA]
BseRI 1231 GAGGAG GAGGAG(10/8)
BsgI 200 GTGCAG GTGCAG(16/14)
BspDI 2325 ATCGAT AT^CGAT
BsrDI 807 GCAATG GCAATG(2/0)
BstUI 49 CGCG CG^CG
BstXI 1486 CCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2325 ATCGAT AT^CGAT
DsaI 2023 CCATGG C^C[GA][CT]GG
Eam1105I 4154 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 497 AGCGCT AGC^GCT
FauI 13 GCGGG CCCGC(4/6)
FbaI 3043 TGATCA T^GATCA
HapII 1032 CCGG C^CGG
HpaI 4880 GTTAAC GTT^AAC
HpaII 1032 CCGG C^CGG
KpnI 1706 GGTACC GGTAC^C
MaeII 4317 ACGT A^CGT
MfeI 317 CAATTG C^AATTG
MspA1I 1193 CAGCTG C[AC]G^C[GT]G
MspI 1032 CCGG C^CGG
MunI 317 CAATTG C^AATTG
MvnI 49 CGCG CG^CG
NcoI 2023 CCATGG C^CATGG
NdeI 2075 CATATG CA^TATG
NheI 19 GCTAGC G^CTAGC
PflMI 4245 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4737 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 225 CTGCAG CTGCA^G
PvuII 1193 CAGCTG CAG^CTG
SphI 2169 GCATGC GCATG^C
SwaI 3315 ATTTAAAT ATTT^AAAT
Van91I 4245 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4245 CCATTTCTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 270 CCCGAG C^[CT]CG[GA]G
3683 CCCGAG
BalI 2250 TGGCCA TGG^CCA
2628 TGGCCA
BbsI 578 GAAGAC GAAGAC(2/6)
3555 GAAGAC
BglII 85 AGATCT A^GATCT
2550 AGATCT
BpuAI 578 GAAGAC GAAGAC(2/6)
3555 GAAGAC
BsaI 246 GAGACC GGTCTC(1/5)
3590 GGTCTC
BsoBI 270 CCCGAG C^[CT]CG[GA]G
3683 CCCGAG
BspHI 822 TCATGA T^CATGA
4796 TCATGA
CfoI 434 GCGC GCG^C
498 GCGC
EaeI 2250 TGGCCA [CT]^GGCC[GA]
2628 TGGCCA
HaeII 433 GGCGCT [GA]GCGC^[CT]
497 AGCGCT
HhaI 434 GCGC GCG^C
498 GCGC
HinP1I 434 GCGC G^CGC
498 GCGC
HincII 1813 GTTGAC GT[CT]^[GA]AC
4880 GTTAAC
HindII 1813 GTTGAC GT[CT]^[GA]AC
4880 GTTAAC
MluNI 2250 TGGCCA TGG^CCA
2628 TGGCCA
MscI 2250 TGGCCA TGG^CCA
2628 TGGCCA
MslI 817 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1016 CACATGCGTG
PpuMI 1323 AGGTCCT [GA]G^G[AT]CC[CT]
4591 AGGACCC
RcaI 822 TCATGA T^CATGA
4796 TCATGA
SpeI 1736 ACTAGT A^CTAGT
4384 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaII BanII
BbvI BfaI BfrI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII