##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI BamHI BbeI BbvCI BcgI
BfrI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI HaeII HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SspI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1925 GGTACC G^GTACC
AccI 2404 GTCTAC GT^[AC][GT]AC
AclI 1141 AACGTT AA^CGTT
AflIII 3119 ACATGT A^C[GA][CT]GT
Alw44I 1326 GTGCAC G^TGCAC
ApaLI 1326 GTGCAC G^TGCAC
Asp718I 1925 GGTACC G^GTACC
AvrII 4058 CCTAGG C^CTAGG
BciVI 3916 GGATAC GTATCC(6/5)
BclI 3683 TGATCA T^GATCA
BcnI 961 CCGGG CC^[GC]GG
BlnI 4058 CCTAGG C^CTAGG
BlpI 2162 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2162 GCTTAGC GC^T[ACGT]AGC
BsmI 2841 GAATGC GAATGC(1/-1)
BspLU11I 3119 ACATGT A^CATGT
BstAPI 1440 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2162 GCTTAGC GC^T[ACGT]AGC
DraIII 1284 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 2560 GATATC GAT^ATC
FbaI 3683 TGATCA T^GATCA
HindIII 928 AAGCTT A^AGCTT
KpnI 1925 GGTACC GGTAC^C
NciI 961 CCGGG CC^[GC]GG
NdeI 1347 CATATG CA^TATG
PshAI 1262 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1141 AACGTT AA^CGTT
SexAI 864 ACCAGGT A^CC[AT]GGT
SpeI 4294 ACTAGT A^CTAGT
SphI 3303 GCATGC GCATG^C
SwaI 4532 ATTTAAAT ATTT^AAAT
XcmI 1865 CCAGGTGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 37 CGCG CG^CG
39 CGCG
AcsI 1400 AAATTC [GA]^AATT[CT]
2859 AAATTT
AcyI 570 GACGCC G[GA]^CG[CT]C
3378 GACGCC
AocI 1271 CCTAAGG CC^T[ACGT]AGG
1665 CCTCAGG
ApoI 1400 AAATTC [GA]^AATT[CT]
2859 AAATTT
Asp700I 1765 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3225 GAACATTTTC
BalI 592 TGGCCA TGG^CCA
2593 TGGCCA
BsaHI 570 GACGCC G[GA]^CG[CT]C
3378 GACGCC
BsrDI 1678 GCAATG GCAATG(2/0)
3537 GCAATG
BstUI 37 CGCG CG^CG
39 CGCG
Bsu36I 1271 CCTAAGG CC^T[ACGT]AGG
1665 CCTCAGG
DraI 3085 TTTAAA TTT^AAA
4533 TTTAAA
EaeI 592 TGGCCA [CT]^GGCC[GA]
2593 TGGCCA
Eco81I 1271 CCTAAGG CC^T[ACGT]AGG
1665 CCTCAGG
Hin1I 570 GACGCC G[GA]^CG[CT]C
3378 GACGCC
HincII 2096 GTCAAC GT[CT]^[GA]AC
3741 GTTGAC
HindII 2096 GTCAAC GT[CT]^[GA]AC
3741 GTTGAC
MluNI 592 TGGCCA TGG^CCA
2593 TGGCCA
MscI 592 TGGCCA TGG^CCA
2593 TGGCCA
MvnI 37 CGCG CG^CG
39 CGCG
NcoI 2956 CCATGG C^CATGG
4467 CCATGG
PaeR7I 431 CTCGAG C^TCGAG
1022 CTCGAG
PstI 278 CTGCAG CTGCA^G
2493 CTGCAG
SacI 784 GAGCTC GAGCT^C
2783 GAGCTC
SapI 781 GAAGAGC GCTCTTC(1/4)
2630 GCTCTTC
XbaI 2426 TCTAGA T^CTAGA
3264 TCTAGA
XhoI 431 CTCGAG C^TCGAG
1022 CTCGAG
XmnI 1765 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3225 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII AviII
BanI BanII BbrPI BbsI BbvI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII