################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccIII AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI BamHI BbeI BbvCI BcgI BfrI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoT22I FseI HaeII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 1925 GGTACC G^GTACC AccI 2404 GTCTAC GT^[AC][GT]AC AclI 1141 AACGTT AA^CGTT AflIII 3119 ACATGT A^C[GA][CT]GT Alw44I 1326 GTGCAC G^TGCAC ApaLI 1326 GTGCAC G^TGCAC Asp718I 1925 GGTACC G^GTACC AvrII 4058 CCTAGG C^CTAGG BciVI 3916 GGATAC GTATCC(6/5) BclI 3683 TGATCA T^GATCA BcnI 961 CCGGG CC^[GC]GG BlnI 4058 CCTAGG C^CTAGG BlpI 2162 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2162 GCTTAGC GC^T[ACGT]AGC BsmI 2841 GAATGC GAATGC(1/-1) BspLU11I 3119 ACATGT A^CATGT BstAPI 1440 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 2162 GCTTAGC GC^T[ACGT]AGC DraIII 1284 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 2560 GATATC GAT^ATC FbaI 3683 TGATCA T^GATCA HindIII 928 AAGCTT A^AGCTT KpnI 1925 GGTACC GGTAC^C NciI 961 CCGGG CC^[GC]GG NdeI 1347 CATATG CA^TATG PshAI 1262 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1141 AACGTT AA^CGTT SexAI 864 ACCAGGT A^CC[AT]GGT SpeI 4294 ACTAGT A^CTAGT SphI 3303 GCATGC GCATG^C SwaI 4532 ATTTAAAT ATTT^AAAT XcmI 1865 CCAGGTGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 37 CGCG CG^CG 39 CGCG AcsI 1400 AAATTC [GA]^AATT[CT] 2859 AAATTT AcyI 570 GACGCC G[GA]^CG[CT]C 3378 GACGCC AocI 1271 CCTAAGG CC^T[ACGT]AGG 1665 CCTCAGG ApoI 1400 AAATTC [GA]^AATT[CT] 2859 AAATTT Asp700I 1765 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3225 GAACATTTTC BalI 592 TGGCCA TGG^CCA 2593 TGGCCA BsaHI 570 GACGCC G[GA]^CG[CT]C 3378 GACGCC BsrDI 1678 GCAATG GCAATG(2/0) 3537 GCAATG BstUI 37 CGCG CG^CG 39 CGCG Bsu36I 1271 CCTAAGG CC^T[ACGT]AGG 1665 CCTCAGG DraI 3085 TTTAAA TTT^AAA 4533 TTTAAA EaeI 592 TGGCCA [CT]^GGCC[GA] 2593 TGGCCA Eco81I 1271 CCTAAGG CC^T[ACGT]AGG 1665 CCTCAGG Hin1I 570 GACGCC G[GA]^CG[CT]C 3378 GACGCC HincII 2096 GTCAAC GT[CT]^[GA]AC 3741 GTTGAC HindII 2096 GTCAAC GT[CT]^[GA]AC 3741 GTTGAC MluNI 592 TGGCCA TGG^CCA 2593 TGGCCA MscI 592 TGGCCA TGG^CCA 2593 TGGCCA MvnI 37 CGCG CG^CG 39 CGCG NcoI 2956 CCATGG C^CATGG 4467 CCATGG PaeR7I 431 CTCGAG C^TCGAG 1022 CTCGAG PstI 278 CTGCAG CTGCA^G 2493 CTGCAG SacI 784 GAGCTC GAGCT^C 2783 GAGCTC SapI 781 GAAGAGC GCTCTTC(1/4) 2630 GCTCTTC XbaI 2426 TCTAGA T^CTAGA 3264 TCTAGA XhoI 431 CTCGAG C^TCGAG 1022 CTCGAG XmnI 1765 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3225 GAACATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII AviII BanI BanII BbrPI BbsI BbvI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII