##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AvrII
BamHI BanI BbeI BbvCI BcgI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BsmBI BsoBI BspDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FseI
HaeII HgaI Hin1I KasI KpnI
KspI MamI MluI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PflFI
PflMI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 2973 CGCG CG^CG
AccIII 1427 TCCGGA T^CCGGA
AviII 3274 TGCGCA TGC^GCA
BalI 2194 TGGCCA TGG^CCA
BbrPI 1436 CACGTG CAC^GTG
BclI 47 TGATCA T^GATCA
BsaWI 1427 TCCGGA [AT]^CCGG[AT]
BseAI 1427 TCCGGA T^CCGGA
BspEI 1427 TCCGGA T^CCGGA
BsrBI 63 GAGCGG CCGCTC(-3/-3)
BsrFI 1850 GCCGGT [GA]^CCGG[CT]
BstAPI 723 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2973 CGCG CG^CG
CfoI 3275 GCGC GCG^C
Cfr10I 1850 GCCGGT [GA]^CCGG[CT]
DraIII 824 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2194 TGGCCA [CT]^GGCC[GA]
FbaI 47 TGATCA T^GATCA
FspI 3274 TGCGCA TGC^GCA
HhaI 3275 GCGC GCG^C
HinP1I 3275 GCGC G^CGC
MfeI 3600 CAATTG C^AATTG
MluNI 2194 TGGCCA TGG^CCA
MroI 1427 TCCGGA T^CCGGA
MscI 2194 TGGCCA TGG^CCA
MunI 3600 CAATTG C^AATTG
MvnI 2973 CGCG CG^CG
NcoI 2982 CCATGG C^CATGG
PacI 2779 TTAATTAA TTAAT^TAA
PmaCI 1436 CACGTG CAC^GTG
PmlI 1436 CACGTG CAC^GTG
PshAI 1405 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1178 GAGCTC GAGCT^C
SexAI 2959 ACCTGGT A^CC[AT]GGT
StuI 2911 AGGCCT AGG^CCT
XbaI 2394 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1601 GTCTAC GT^[AC][GT]AC
2031 GTCTAC
BanII 1178 GAGCTC G[GA]GC[CT]^C
2471 GGGCTC
BciVI 2987 GTATCC GTATCC(6/5)
3660 GTATCC
BsaAI 1436 CACGTG [CT]AC^GT[GA]
4129 CACGTA
BsaI 95 GAGACC GGTCTC(1/5)
1169 GAGACC
BsmI 1314 GAATGC GAATGC(1/-1)
3766 GCATTC
Bsp1407I 1559 TGTACA T^GTACA
1942 TGTACA
BspHI 1128 TCATGA T^CATGA
3480 TCATGA
BspLU11I 197 ACATGT A^CATGT
3121 ACATGT
BsrGI 1559 TGTACA T^GTACA
1942 TGTACA
BstXI 3119 CCACATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3414 CCAACCAAATGG
DraII 742 AGGACCC [GA]G^G[ACGT]CC[CT]
3093 GGGACCT
DsaI 132 CCACGG C^C[GA][CT]GG
2982 CCATGG
EcoO109I 742 AGGACCC [GA]G^G[ACGT]CC[CT]
3093 GGGACCT
EcoRV 178 GATATC GAT^ATC
2421 GATATC
FauI 1795 CCCGC CCCGC(4/6)
65 GCGGG
HapII 1428 CCGG C^CGG
1851 CCGG
HincII 3945 GTTAAC GT[CT]^[GA]AC
3962 GTTAAC
HindII 3945 GTTAAC GT[CT]^[GA]AC
3962 GTTAAC
HpaI 3945 GTTAAC GTT^AAC
3962 GTTAAC
HpaII 1428 CCGG C^CGG
1851 CCGG
MaeII 1437 ACGT A^CGT
4130 ACGT
MspA1I 448 CAGCTG C[AC]G^C[GT]G
2306 CAGCTG
MspI 1428 CCGG C^CGG
1851 CCGG
PpuMI 742 AGGACCC [GA]G^G[AT]CC[CT]
3093 GGGACCT
PstI 264 CTGCAG CTGCA^G
2454 CTGCAG
PvuII 448 CAGCTG CAG^CTG
2306 CAGCTG
RcaI 1128 TCATGA T^CATGA
3480 TCATGA
SspBI 1559 TGTACA T^GTACA
1942 TGTACA
XcmI 541 CCAGCAGAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3042 CCAATGGATTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI AseI AsnI Asp700I AspHI
AvaII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI