################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AvrII BamHI BanI BbeI BbvCI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI BsoBI BspDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI HaeII HgaI Hin1I KasI KpnI KspI MamI MluI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflFI PflMI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 2973 CGCG CG^CG AccIII 1427 TCCGGA T^CCGGA AviII 3274 TGCGCA TGC^GCA BalI 2194 TGGCCA TGG^CCA BbrPI 1436 CACGTG CAC^GTG BclI 47 TGATCA T^GATCA BsaWI 1427 TCCGGA [AT]^CCGG[AT] BseAI 1427 TCCGGA T^CCGGA BspEI 1427 TCCGGA T^CCGGA BsrBI 63 GAGCGG CCGCTC(-3/-3) BsrFI 1850 GCCGGT [GA]^CCGG[CT] BstAPI 723 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 2973 CGCG CG^CG CfoI 3275 GCGC GCG^C Cfr10I 1850 GCCGGT [GA]^CCGG[CT] DraIII 824 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2194 TGGCCA [CT]^GGCC[GA] FbaI 47 TGATCA T^GATCA FspI 3274 TGCGCA TGC^GCA HhaI 3275 GCGC GCG^C HinP1I 3275 GCGC G^CGC MfeI 3600 CAATTG C^AATTG MluNI 2194 TGGCCA TGG^CCA MroI 1427 TCCGGA T^CCGGA MscI 2194 TGGCCA TGG^CCA MunI 3600 CAATTG C^AATTG MvnI 2973 CGCG CG^CG NcoI 2982 CCATGG C^CATGG PacI 2779 TTAATTAA TTAAT^TAA PmaCI 1436 CACGTG CAC^GTG PmlI 1436 CACGTG CAC^GTG PshAI 1405 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1178 GAGCTC GAGCT^C SexAI 2959 ACCTGGT A^CC[AT]GGT StuI 2911 AGGCCT AGG^CCT XbaI 2394 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1601 GTCTAC GT^[AC][GT]AC 2031 GTCTAC BanII 1178 GAGCTC G[GA]GC[CT]^C 2471 GGGCTC BciVI 2987 GTATCC GTATCC(6/5) 3660 GTATCC BsaAI 1436 CACGTG [CT]AC^GT[GA] 4129 CACGTA BsaI 95 GAGACC GGTCTC(1/5) 1169 GAGACC BsmI 1314 GAATGC GAATGC(1/-1) 3766 GCATTC Bsp1407I 1559 TGTACA T^GTACA 1942 TGTACA BspHI 1128 TCATGA T^CATGA 3480 TCATGA BspLU11I 197 ACATGT A^CATGT 3121 ACATGT BsrGI 1559 TGTACA T^GTACA 1942 TGTACA BstXI 3119 CCACATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3414 CCAACCAAATGG DraII 742 AGGACCC [GA]G^G[ACGT]CC[CT] 3093 GGGACCT DsaI 132 CCACGG C^C[GA][CT]GG 2982 CCATGG EcoO109I 742 AGGACCC [GA]G^G[ACGT]CC[CT] 3093 GGGACCT EcoRV 178 GATATC GAT^ATC 2421 GATATC FauI 1795 CCCGC CCCGC(4/6) 65 GCGGG HapII 1428 CCGG C^CGG 1851 CCGG HincII 3945 GTTAAC GT[CT]^[GA]AC 3962 GTTAAC HindII 3945 GTTAAC GT[CT]^[GA]AC 3962 GTTAAC HpaI 3945 GTTAAC GTT^AAC 3962 GTTAAC HpaII 1428 CCGG C^CGG 1851 CCGG MaeII 1437 ACGT A^CGT 4130 ACGT MspA1I 448 CAGCTG C[AC]G^C[GT]G 2306 CAGCTG MspI 1428 CCGG C^CGG 1851 CCGG PpuMI 742 AGGACCC [GA]G^G[AT]CC[CT] 3093 GGGACCT PstI 264 CTGCAG CTGCA^G 2454 CTGCAG PvuII 448 CAGCTG CAG^CTG 2306 CAGCTG RcaI 1128 TCATGA T^CATGA 3480 TCATGA SspBI 1559 TGTACA T^GTACA 1942 TGTACA XcmI 541 CCAGCAGAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3042 CCAATGGATTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI ApoI AseI AsnI Asp700I AspHI AvaII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI