################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AccI AccIII AflII AgeI AhdI Alw44I ApaLI AscI AspEI AspI AviII AvrII BalI BbrPI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraIII DrdI Eam1105I EcoO65I EcoRV FseI FspI HincII HindII HpaI MamI MluNI MroI MscI NdeI NspV PflFI PinAI PmaCI PmeI PmlI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SnaBI SphI Sse8387I StuI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 67 GACGTC GACGT^C Acc65I 319 GGTACC G^GTACC AclI 1444 AACGTT AA^CGTT AlwNI 306 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1130 AGCGCT AGC^GCT Asp718I 319 GGTACC G^GTACC BamHI 1996 GGATCC G^GATCC BbeI 290 GGCGCC GGCGC^C BbvCI 760 CCTCAGC CCTCAGC(-5/-2) BcgI 2679 GCAACTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3546 TGATCA T^GATCA BglII 2955 AGATCT A^GATCT BsgI 95 CTGCAC GTGCAG(16/14) BspMI 492 ACCTGC ACCTGC(4/8) BssHII 405 GCGCGC G^CGCGC Eco47III 1130 AGCGCT AGC^GCT EcoNI 3583 CCTGTCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3017 GAATTC G^AATTC EcoT22I 3164 ATGCAT ATGCA^T FbaI 3546 TGATCA T^GATCA KasI 290 GGCGCC G^GCGCC KpnI 319 GGTACC GGTAC^C MfeI 1289 CAATTG C^AATTG MluI 2370 ACGCGT A^CGCGT MunI 1289 CAATTG C^AATTG NaeI 744 GCCGGC GCC^GGC NarI 290 GGCGCC GG^CGCC NcoI 196 CCATGG C^CATGG NgoMIV 744 GCCGGC G^CCGGC NheI 3595 GCTAGC G^CTAGC NotI 330 GCGGCCGC GC^GGCCGC NruI 1723 TCGCGA TCG^CGA NsiI 3164 ATGCAT ATGCA^T PacI 1527 TTAATTAA TTAAT^TAA PshAI 2144 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1444 AACGTT AA^CGTT PstI 312 CTGCAG CTGCA^G PvuII 2099 CAGCTG CAG^CTG SacI 2710 GAGCTC GAGCT^C SapI 2210 GAAGAGC GCTCTTC(1/4) SfoI 290 GGCGCC GGC^GCC SgrAI 743 CGCCGGCG C[GA]^CCGG[CT]G SpeI 3899 ACTAGT A^CTAGT SspI 3680 AATATT AAT^ATT XcmI 905 CCATAGTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 3496 CCTTAGG CC^T[ACGT]AGG 3558 CCTCAGG ApaI 192 GGGCCC GGGCC^C 338 GGGCCC AspHI 910 GTGCTC G[AT]GC[AT]^C 2710 GAGCTC BglI 296 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 648 GCCGGGAGGGC BsiHKAI 910 GTGCTC G[AT]GC[AT]^C 2710 GAGCTC BspLU11I 1626 ACATGT A^CATGT 2626 ACATGT BsrBI 570 CCGCTC CCGCTC(-3/-3) 833 GAGCGG BsrFI 744 GCCGGC [GA]^CCGG[CT] 1141 ACCGGC BstAPI 305 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1337 GCAGGGATTGC Bsu36I 3496 CCTTAGG CC^T[ACGT]AGG 3558 CCTCAGG Cfr10I 744 GCCGGC [GA]^CCGG[CT] 1141 ACCGGC Eco81I 3496 CCTTAGG CC^T[ACGT]AGG 3558 CCTCAGG EcoT14I 196 CCATGG C^C[AT][AT]GG 2823 CCTTGG HindIII 1454 AAGCTT A^AGCTT 2091 AAGCTT HphI 725 GGTGA GGTGA(8/7) 1139 TCACC MslI 1621 CATATACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2114 CATTCAGATG NspI 1626 ACATGT [GA]CATG^[CT] 2626 ACATGT PaeR7I 573 CTCGAG C^TCGAG 2753 CTCGAG PflMI 2134 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2457 CCATAATTTGG SmlI 573 CTCGAG C^T[CT][GA]AG 2753 CTCGAG StyI 196 CCATGG C^C[AT][AT]GG 2823 CCTTGG Tsp45I 1138 GTCAC ^GT[GC]AC 2231 GTGAC Van91I 2134 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2457 CCATAATTTGG XbaI 2663 TCTAGA T^CTAGA 3640 TCTAGA XhoI 573 CTCGAG C^TCGAG 2753 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AseI AsnI Asp700I AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PshBI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SspBI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmaCI XmaI XmnI