##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccI AccIII AflII AgeI AhdI
Alw44I ApaLI AscI AspEI AspI
AviII AvrII BalI BbrPI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI Eam1105I EcoO65I EcoRV
FseI FspI HincII HindII HpaI
MamI MluNI MroI MscI NdeI
NspV PflFI PinAI PmaCI PmeI
PmlI PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SnaBI SphI Sse8387I StuI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 67 GACGTC GACGT^C
Acc65I 319 GGTACC G^GTACC
AclI 1444 AACGTT AA^CGTT
AlwNI 306 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1130 AGCGCT AGC^GCT
Asp718I 319 GGTACC G^GTACC
BamHI 1996 GGATCC G^GATCC
BbeI 290 GGCGCC GGCGC^C
BbvCI 760 CCTCAGC CCTCAGC(-5/-2)
BcgI 2679 GCAACTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3546 TGATCA T^GATCA
BglII 2955 AGATCT A^GATCT
BsgI 95 CTGCAC GTGCAG(16/14)
BspMI 492 ACCTGC ACCTGC(4/8)
BssHII 405 GCGCGC G^CGCGC
Eco47III 1130 AGCGCT AGC^GCT
EcoNI 3583 CCTGTCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3017 GAATTC G^AATTC
EcoT22I 3164 ATGCAT ATGCA^T
FbaI 3546 TGATCA T^GATCA
KasI 290 GGCGCC G^GCGCC
KpnI 319 GGTACC GGTAC^C
MfeI 1289 CAATTG C^AATTG
MluI 2370 ACGCGT A^CGCGT
MunI 1289 CAATTG C^AATTG
NaeI 744 GCCGGC GCC^GGC
NarI 290 GGCGCC GG^CGCC
NcoI 196 CCATGG C^CATGG
NgoMIV 744 GCCGGC G^CCGGC
NheI 3595 GCTAGC G^CTAGC
NotI 330 GCGGCCGC GC^GGCCGC
NruI 1723 TCGCGA TCG^CGA
NsiI 3164 ATGCAT ATGCA^T
PacI 1527 TTAATTAA TTAAT^TAA
PshAI 2144 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1444 AACGTT AA^CGTT
PstI 312 CTGCAG CTGCA^G
PvuII 2099 CAGCTG CAG^CTG
SacI 2710 GAGCTC GAGCT^C
SapI 2210 GAAGAGC GCTCTTC(1/4)
SfoI 290 GGCGCC GGC^GCC
SgrAI 743 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3899 ACTAGT A^CTAGT
SspI 3680 AATATT AAT^ATT
XcmI 905 CCATAGTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 3496 CCTTAGG CC^T[ACGT]AGG
3558 CCTCAGG
ApaI 192 GGGCCC GGGCC^C
338 GGGCCC
AspHI 910 GTGCTC G[AT]GC[AT]^C
2710 GAGCTC
BglI 296 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
648 GCCGGGAGGGC
BsiHKAI 910 GTGCTC G[AT]GC[AT]^C
2710 GAGCTC
BspLU11I 1626 ACATGT A^CATGT
2626 ACATGT
BsrBI 570 CCGCTC CCGCTC(-3/-3)
833 GAGCGG
BsrFI 744 GCCGGC [GA]^CCGG[CT]
1141 ACCGGC
BstAPI 305 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1337 GCAGGGATTGC
Bsu36I 3496 CCTTAGG CC^T[ACGT]AGG
3558 CCTCAGG
Cfr10I 744 GCCGGC [GA]^CCGG[CT]
1141 ACCGGC
Eco81I 3496 CCTTAGG CC^T[ACGT]AGG
3558 CCTCAGG
EcoT14I 196 CCATGG C^C[AT][AT]GG
2823 CCTTGG
HindIII 1454 AAGCTT A^AGCTT
2091 AAGCTT
HphI 725 GGTGA GGTGA(8/7)
1139 TCACC
MslI 1621 CATATACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2114 CATTCAGATG
NspI 1626 ACATGT [GA]CATG^[CT]
2626 ACATGT
PaeR7I 573 CTCGAG C^TCGAG
2753 CTCGAG
PflMI 2134 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2457 CCATAATTTGG
SmlI 573 CTCGAG C^T[CT][GA]AG
2753 CTCGAG
StyI 196 CCATGG C^C[AT][AT]GG
2823 CCTTGG
Tsp45I 1138 GTCAC ^GT[GC]AC
2231 GTGAC
Van91I 2134 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2457 CCATAATTTGG
XbaI 2663 TCTAGA T^CTAGA
3640 TCTAGA
XhoI 573 CTCGAG C^TCGAG
2753 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PshBI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SspBI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI XmnI