##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
Asp700I AviII BbeI BbrPI BfrI
BglII BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI BsmI BspDI BspHI
BsrFI BssHII BssSI BstBI Bsu36I
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FseI FspI Hin1I HpaI KasI
KspI MamI MfeI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacI SacII SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 4162 GGTACC G^GTACC
AccII 27 CGCG CG^CG
AhdI 3561 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3951 ATTAAT AT^TAAT
AsnI 3951 ATTAAT AT^TAAT
Asp718I 4162 GGTACC G^GTACC
AspEI 3561 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3772 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2076 CTCGGG C^[CT]CG[GA]G
AvrII 4628 CCTAGG C^CTAGG
BcgI 2215 CGACACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 842 TGATCA T^GATCA
BglI 3820 GCCATCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4628 CCTAGG C^CTAGG
BsoBI 2076 CTCGGG C^[CT]CG[GA]G
Bsp1407I 4052 TGTACA T^GTACA
BspLU11I 3511 ACATGT A^CATGT
BspMI 4083 ACCTGC ACCTGC(4/8)
BsrBI 2363 GAGCGG CCGCTC(-3/-3)
BsrGI 4052 TGTACA T^GTACA
BstEII 4472 GGTCACC G^GT[ACGT]ACC
BstPI 4472 GGTCACC G^GT[ACGT]ACC
BstUI 27 CGCG CG^CG
DraIII 2162 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1777 GACAGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3561 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4472 GGTCACC G^GT[ACGT]ACC
EcoRV 397 GATATC GAT^ATC
FbaI 842 TGATCA T^GATCA
HgaI 28 GCGTC GACGC(5/10)
HindIII 1372 AAGCTT A^AGCTT
KpnI 4162 GGTACC GGTAC^C
MluI 26 ACGCGT A^CGCGT
MvnI 27 CGCG CG^CG
NheI 313 GCTAGC G^CTAGC
PflFI 3772 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3951 ATTAAT AT^TAAT
SalI 3769 GTCGAC G^TCGAC
SexAI 715 ACCTGGT A^CC[AT]GGT
SfiI 3819 GGCCATCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4428 ACTAGT A^CTAGT
SspBI 4052 TGTACA T^GTACA
Tth111I 3772 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4548 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 262 TCCGGA T^CCGGA
4390 TCCGGA
AflIII 26 ACGCGT A^C[GA][CT]GT
3511 ACATGT
ApaI 3679 GGGCCC GGGCC^C
4576 GGGCCC
BamHI 1351 GGATCC G^GATCC
1898 GGATCC
BbvCI 2460 GCTGAGG CCTCAGC(-5/-2)
3252 GCTGAGG
BciVI 3507 GGATAC GTATCC(6/5)
1189 GTATCC
BlpI 3728 GCTTAGC GC^T[ACGT]AGC
4256 GCTTAGC
Bpu1102I 3728 GCTTAGC GC^T[ACGT]AGC
4256 GCTTAGC
BsaAI 105 TACGTG [CT]AC^GT[GA]
325 CACGTA
BseAI 262 TCCGGA T^CCGGA
4390 TCCGGA
BseRI 2446 CTCCTC GAGGAG(10/8)
4077 CTCCTC
BspEI 262 TCCGGA T^CCGGA
4390 TCCGGA
Bst1107I 3872 GTATAC GTA^TAC
4728 GTATAC
BstXI 575 CCAGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2728 CCAGGACTGTGG
BstYI 1351 GGATCC [GA]^GATC[CT]
1898 GGATCC
BstZ17I 3872 GTATAC GTA^TAC
4728 GTATAC
CelII 3728 GCTTAGC GC^T[ACGT]AGC
4256 GCTTAGC
CfoI 1956 GCGC GCG^C
3322 GCGC
DraI 903 TTTAAA TTT^AAA
4750 TTTAAA
DsaI 3102 CCATGG C^C[GA][CT]GG
3240 CCATGG
HaeII 1955 GGCGCT [GA]GCGC^[CT]
3321 GGCGCT
HhaI 1956 GCGC GCG^C
3322 GCGC
HinP1I 1956 GCGC G^CGC
3322 GCGC
MflI 1351 GGATCC [GA]^GATC[CT]
1898 GGATCC
MroI 262 TCCGGA T^CCGGA
4390 TCCGGA
MslI 383 CATTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4243 CATCAGAATG
NcoI 3102 CCATGG C^CATGG
3240 CCATGG
NspI 3511 ACATGT [GA]CATG^[CT]
4715 GCATGT
PshAI 2732 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4091 GACAACCGTC
SapI 1197 GCTCTTC GCTCTTC(1/4)
3759 GCTCTTC
XhoII 1351 GGATCC [GA]^GATC[CT]
1898 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI