################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccII AclI AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI BamHI BcgI BciVI BglII BsaBI BsiEI BsiWI BssHII BstBI BstUI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoT22I FseI HindIII HpaI KspI MamI MfeI MluI MunI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfuI SgrAI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 2144 GACGTC GACGT^C Acc65I 4258 GGTACC G^GTACC AcsI 4411 AAATTT [GA]^AATT[CT] Alw44I 4138 GTGCAC G^TGCAC ApaLI 4138 GTGCAC G^TGCAC ApoI 4411 AAATTT [GA]^AATT[CT] Asp700I 3476 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4258 GGTACC G^GTACC AviII 4008 TGCGCA TGC^GCA BbeI 2017 GGCGCC GGCGC^C BclI 767 TGATCA T^GATCA BglI 3190 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BspDI 3633 ATCGAT AT^CGAT BspHI 1250 TCATGA T^CATGA BssSI 2404 CTCGTG CACGAG(-5/-1) Bst1107I 4684 GTATAC GTA^TAC BstAPI 1552 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 4684 GTATAC GTA^TAC ClaI 3633 ATCGAT AT^CGAT DraIII 3486 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 4655 GATATC GAT^ATC FbaI 767 TGATCA T^GATCA FspI 4008 TGCGCA TGC^GCA KasI 2017 GGCGCC G^GCGCC KpnI 4258 GGTACC GGTAC^C NarI 2017 GGCGCC GG^CGCC NdeI 617 CATATG CA^TATG PshAI 868 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1250 TCATGA T^CATGA SapI 126 GAAGAGC GCTCTTC(1/4) SexAI 1511 ACCAGGT A^CC[AT]GGT SfiI 3189 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2017 GGCGCC GGC^GCC SmaI 256 CCCGGG CCC^GGG SnaBI 3426 TACGTA TAC^GTA SpeI 1987 ACTAGT A^CTAGT SspI 820 AATATT AAT^ATT XmaCI 256 CCCGGG C^CCGGG XmaI 256 CCCGGG C^CCGGG XmnI 3476 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccIII 15 TCCGGA T^CCGGA 1195 TCCGGA AflII 3142 CTTAAG C^TTAAG 4100 CTTAAG BfrI 3142 CTTAAG C^TTAAG 4100 CTTAAG BseAI 15 TCCGGA T^CCGGA 1195 TCCGGA BsmBI 159 CGTCTC CGTCTC(1/5) 94 GAGACG Bsp1407I 1676 TGTACA T^GTACA 4449 TGTACA BspEI 15 TCCGGA T^CCGGA 1195 TCCGGA BspLU11I 437 ACATGT A^CATGT 2336 ACATGT BsrBI 1800 GAGCGG CCGCTC(-3/-3) 2317 GAGCGG BsrFI 2038 GCCGGT [GA]^CCGG[CT] 3549 GCCGGT BsrGI 1676 TGTACA T^GTACA 4449 TGTACA BstEII 2470 GGTAACC G^GT[ACGT]ACC 4189 GGTAACC BstPI 2470 GGTAACC G^GT[ACGT]ACC 4189 GGTAACC Cfr10I 2038 GCCGGT [GA]^CCGG[CT] 3549 GCCGGT EcoO65I 2470 GGTAACC G^GT[ACGT]ACC 4189 GGTAACC HaeII 2017 GGCGCC [GA]GCGC^[CT] 3786 GGCGCT HgaI 96 GACGC GACGC(5/10) 885 GACGC MroI 15 TCCGGA T^CCGGA 1195 TCCGGA PflMI 1281 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3485 CCACCACGTGG SspBI 1676 TGTACA T^GTACA 4449 TGTACA Tsp45I 3880 GTCAC ^GT[GC]AC 4312 GTGAC Van91I 1281 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3485 CCACCACGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccI AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII AvrII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII