##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
BamHI BcgI BciVI BglII BsaBI
BsiEI BsiWI BssHII BstBI BstUI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI HindIII HpaI KspI MamI
MfeI MluI MunI MvnI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfuI
SgrAI Sse8387I SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2144 GACGTC GACGT^C
Acc65I 4258 GGTACC G^GTACC
AcsI 4411 AAATTT [GA]^AATT[CT]
Alw44I 4138 GTGCAC G^TGCAC
ApaLI 4138 GTGCAC G^TGCAC
ApoI 4411 AAATTT [GA]^AATT[CT]
Asp700I 3476 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4258 GGTACC G^GTACC
AviII 4008 TGCGCA TGC^GCA
BbeI 2017 GGCGCC GGCGC^C
BclI 767 TGATCA T^GATCA
BglI 3190 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BspDI 3633 ATCGAT AT^CGAT
BspHI 1250 TCATGA T^CATGA
BssSI 2404 CTCGTG CACGAG(-5/-1)
Bst1107I 4684 GTATAC GTA^TAC
BstAPI 1552 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4684 GTATAC GTA^TAC
ClaI 3633 ATCGAT AT^CGAT
DraIII 3486 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 4655 GATATC GAT^ATC
FbaI 767 TGATCA T^GATCA
FspI 4008 TGCGCA TGC^GCA
KasI 2017 GGCGCC G^GCGCC
KpnI 4258 GGTACC GGTAC^C
NarI 2017 GGCGCC GG^CGCC
NdeI 617 CATATG CA^TATG
PshAI 868 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1250 TCATGA T^CATGA
SapI 126 GAAGAGC GCTCTTC(1/4)
SexAI 1511 ACCAGGT A^CC[AT]GGT
SfiI 3189 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2017 GGCGCC GGC^GCC
SmaI 256 CCCGGG CCC^GGG
SnaBI 3426 TACGTA TAC^GTA
SpeI 1987 ACTAGT A^CTAGT
SspI 820 AATATT AAT^ATT
XmaCI 256 CCCGGG C^CCGGG
XmaI 256 CCCGGG C^CCGGG
XmnI 3476 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 15 TCCGGA T^CCGGA
1195 TCCGGA
AflII 3142 CTTAAG C^TTAAG
4100 CTTAAG
BfrI 3142 CTTAAG C^TTAAG
4100 CTTAAG
BseAI 15 TCCGGA T^CCGGA
1195 TCCGGA
BsmBI 159 CGTCTC CGTCTC(1/5)
94 GAGACG
Bsp1407I 1676 TGTACA T^GTACA
4449 TGTACA
BspEI 15 TCCGGA T^CCGGA
1195 TCCGGA
BspLU11I 437 ACATGT A^CATGT
2336 ACATGT
BsrBI 1800 GAGCGG CCGCTC(-3/-3)
2317 GAGCGG
BsrFI 2038 GCCGGT [GA]^CCGG[CT]
3549 GCCGGT
BsrGI 1676 TGTACA T^GTACA
4449 TGTACA
BstEII 2470 GGTAACC G^GT[ACGT]ACC
4189 GGTAACC
BstPI 2470 GGTAACC G^GT[ACGT]ACC
4189 GGTAACC
Cfr10I 2038 GCCGGT [GA]^CCGG[CT]
3549 GCCGGT
EcoO65I 2470 GGTAACC G^GT[ACGT]ACC
4189 GGTAACC
HaeII 2017 GGCGCC [GA]GCGC^[CT]
3786 GGCGCT
HgaI 96 GACGC GACGC(5/10)
885 GACGC
MroI 15 TCCGGA T^CCGGA
1195 TCCGGA
PflMI 1281 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3485 CCACCACGTGG
SspBI 1676 TGTACA T^GTACA
4449 TGTACA
Tsp45I 3880 GTCAC ^GT[GC]AC
4312 GTGAC
Van91I 1281 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3485 CCACCACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI XhoII