##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BcgI
BclI BlnI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssSI BstBI BstEII
BstPI ClaI CpoI Eco47III EcoO65I
FbaI FseI HindIII KpnI MamI
MfeI MroI MunI NdeI NheI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SfiI SfuI SnaBI SpeI SspBI
SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3846 GACGTC GACGT^C
AflII 1368 CTTAAG C^TTAAG
AviII 2969 TGCGCA TGC^GCA
BbrPI 3498 CACGTG CAC^GTG
BfrI 1368 CTTAAG C^TTAAG
BsaAI 3498 CACGTG [CT]AC^GT[GA]
BsmBI 2490 CGTCTC CGTCTC(1/5)
Bst1107I 4044 GTATAC GTA^TAC
BstZ17I 4044 GTATAC GTA^TAC
DraI 3924 TTTAAA TTT^AAA
DrdI 1611 GACTTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 771 GAATTC G^AATTC
EcoT22I 913 ATGCAT ATGCA^T
FspI 2969 TGCGCA TGC^GCA
HpaI 1921 GTTAAC GTT^AAC
MluI 1431 ACGCGT A^CGCGT
NcoI 2834 CCATGG C^CATGG
NotI 2743 GCGGCCGC GC^GGCCGC
NruI 2400 TCGCGA TCG^CGA
NsiI 913 ATGCAT ATGCA^T
PaeR7I 3134 CTCGAG C^TCGAG
PflMI 250 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3498 CACGTG CAC^GTG
PmlI 3498 CACGTG CAC^GTG
SacI 149 GAGCTC GAGCT^C
SbfI 2613 CCTGCAGG CCTGCA^GG
ScaI 1957 AGTACT AGT^ACT
SexAI 1597 ACCAGGT A^CC[AT]GGT
Sse8387I 2613 CCTGCAGG CCTGCA^GG
Van91I 250 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1489 TCTAGA T^CTAGA
XcmI 3259 CCAGTCCCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3134 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 771 GAATTC [GA]^AATT[CT]
1374 AAATTC
AflIII 1431 ACGCGT A^C[GA][CT]GT
3499 ACGTGT
ApoI 771 GAATTC [GA]^AATT[CT]
1374 AAATTC
BciVI 1081 GTATCC GTATCC(6/5)
1837 GTATCC
BglII 954 AGATCT A^GATCT
3479 AGATCT
BlpI 428 GCTTAGC GC^T[ACGT]AGC
2249 GCTCAGC
Bpu1102I 428 GCTTAGC GC^T[ACGT]AGC
2249 GCTCAGC
BsmI 3752 GAATGC GAATGC(1/-1)
915 GCATTC
BspHI 284 TCATGA T^CATGA
1859 TCATGA
CelII 428 GCTTAGC GC^T[ACGT]AGC
2249 GCTCAGC
DraIII 3600 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3992 CACTGAGTG
EcoRV 32 GATATC GAT^ATC
3908 GATATC
KspI 3046 CCGCGG CCGC^GG
3340 CCGCGG
RcaI 284 TCATGA T^CATGA
1859 TCATGA
SacII 3046 CCGCGG CCGC^GG
3340 CCGCGG
SalI 2089 GTCGAC G^TCGAC
3849 GTCGAC
SapI 2352 GAAGAGC GCTCTTC(1/4)
3355 GAAGAGC
SmaI 2013 CCCGGG CCC^GGG
3104 CCCGGG
SphI 3473 GCATGC GCATG^C
3635 GCATGC
StuI 415 AGGCCT AGG^CCT
2483 AGGCCT
XmaCI 2013 CCCGGG C^CCGGG
3104 CCCGGG
XmaI 2013 CCCGGG C^CCGGG
3104 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI AspEI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SgrAI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII