################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## Acc65I AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvrII BcgI BclI BlnI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssSI BstBI BstEII BstPI ClaI CpoI Eco47III EcoO65I FbaI FseI HindIII KpnI MamI MfeI MroI MunI NdeI NheI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SfiI SfuI SnaBI SpeI SspBI SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 3846 GACGTC GACGT^C AflII 1368 CTTAAG C^TTAAG AviII 2969 TGCGCA TGC^GCA BbrPI 3498 CACGTG CAC^GTG BfrI 1368 CTTAAG C^TTAAG BsaAI 3498 CACGTG [CT]AC^GT[GA] BsmBI 2490 CGTCTC CGTCTC(1/5) Bst1107I 4044 GTATAC GTA^TAC BstZ17I 4044 GTATAC GTA^TAC DraI 3924 TTTAAA TTT^AAA DrdI 1611 GACTTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 771 GAATTC G^AATTC EcoT22I 913 ATGCAT ATGCA^T FspI 2969 TGCGCA TGC^GCA HpaI 1921 GTTAAC GTT^AAC MluI 1431 ACGCGT A^CGCGT NcoI 2834 CCATGG C^CATGG NotI 2743 GCGGCCGC GC^GGCCGC NruI 2400 TCGCGA TCG^CGA NsiI 913 ATGCAT ATGCA^T PaeR7I 3134 CTCGAG C^TCGAG PflMI 250 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3498 CACGTG CAC^GTG PmlI 3498 CACGTG CAC^GTG SacI 149 GAGCTC GAGCT^C SbfI 2613 CCTGCAGG CCTGCA^GG ScaI 1957 AGTACT AGT^ACT SexAI 1597 ACCAGGT A^CC[AT]GGT Sse8387I 2613 CCTGCAGG CCTGCA^GG Van91I 250 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1489 TCTAGA T^CTAGA XcmI 3259 CCAGTCCCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3134 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 771 GAATTC [GA]^AATT[CT] 1374 AAATTC AflIII 1431 ACGCGT A^C[GA][CT]GT 3499 ACGTGT ApoI 771 GAATTC [GA]^AATT[CT] 1374 AAATTC BciVI 1081 GTATCC GTATCC(6/5) 1837 GTATCC BglII 954 AGATCT A^GATCT 3479 AGATCT BlpI 428 GCTTAGC GC^T[ACGT]AGC 2249 GCTCAGC Bpu1102I 428 GCTTAGC GC^T[ACGT]AGC 2249 GCTCAGC BsmI 3752 GAATGC GAATGC(1/-1) 915 GCATTC BspHI 284 TCATGA T^CATGA 1859 TCATGA CelII 428 GCTTAGC GC^T[ACGT]AGC 2249 GCTCAGC DraIII 3600 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3992 CACTGAGTG EcoRV 32 GATATC GAT^ATC 3908 GATATC KspI 3046 CCGCGG CCGC^GG 3340 CCGCGG RcaI 284 TCATGA T^CATGA 1859 TCATGA SacII 3046 CCGCGG CCGC^GG 3340 CCGCGG SalI 2089 GTCGAC G^TCGAC 3849 GTCGAC SapI 2352 GAAGAGC GCTCTTC(1/4) 3355 GAAGAGC SmaI 2013 CCCGGG CCC^GGG 3104 CCCGGG SphI 3473 GCATGC GCATG^C 3635 GCATGC StuI 415 AGGCCT AGG^CCT 2483 AGGCCT XmaCI 2013 CCCGGG C^CCGGG 3104 CCCGGG XmaI 2013 CCCGGG C^CCGGG 3104 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApaI AspEI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SgrAI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII