##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I Alw44I Aor51HI ApaLI AscI
Asp718I BbrPI BlpI Bpu1102I BsaAI
BsiEI BsiWI BsmBI BspLU11I BssHII
Bst1107I BstBI BstZ17I CelII CpoI
EagI EclXI Eco47III Eco52I EcoRV
FseI HpaI KpnI KspI MluI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI PvuI RsrII SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AatII 2488 GACGTC GACGT^C
AccI 1160 GTAGAC GT^[AC][GT]AC
AccII 1355 CGCG CG^CG
AclI 2987 AACGTT AA^CGTT
AgeI 2970 ACCGGT A^CCGGT
AhdI 3211 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2601 GGGCCC GGGCC^C
AseI 3445 ATTAAT AT^TAAT
AsnI 3445 ATTAAT AT^TAAT
AspEI 3211 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 287 TGCGCA TGC^GCA
BalI 3279 TGGCCA TGG^CCA
BamHI 4035 GGATCC G^GATCC
BbeI 498 GGCGCC GGCGC^C
BbvCI 992 GCTGAGG CCTCAGC(-5/-2)
BcgI 290 GCACCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 616 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI 1374 GAATGC GAATGC(1/-1)
Bsp1407I 649 TGTACA T^GTACA
BspDI 279 ATCGAT AT^CGAT
BsrFI 2970 ACCGGT [GA]^CCGG[CT]
BsrGI 649 TGTACA T^GTACA
BssSI 963 CACGAG CACGAG(-5/-1)
BstUI 1355 CGCG CG^CG
BstXI 121 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2970 ACCGGT [GA]^CCGG[CT]
ClaI 279 ATCGAT AT^CGAT
DraI 3787 TTTAAA TTT^AAA
DraIII 4006 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1319 GACGTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3211 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3258 GAATTC G^AATTC
EcoT22I 275 ATGCAT ATGCA^T
FspI 287 TGCGCA TGC^GCA
HgaI 1436 GACGC GACGC(5/10)
HincII 3410 GTTGAC GT[CT]^[GA]AC
HindII 3410 GTTGAC GT[CT]^[GA]AC
KasI 498 GGCGCC G^GCGCC
MfeI 3250 CAATTG C^AATTG
MluNI 3279 TGGCCA TGG^CCA
MscI 3279 TGGCCA TGG^CCA
MunI 3250 CAATTG C^AATTG
MvnI 1355 CGCG CG^CG
NarI 498 GGCGCC GG^CGCC
NcoI 604 CCATGG C^CATGG
NheI 2773 GCTAGC G^CTAGC
NsiI 275 ATGCAT ATGCA^T
PflMI 2723 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2970 ACCGGT A^CCGGT
PshAI 1908 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3445 ATTAAT AT^TAAT
Psp1406I 2987 AACGTT AA^CGTT
SacI 948 GAGCTC GAGCT^C
SbfI 1419 CCTGCAGG CCTGCA^GG
SfoI 498 GGCGCC GGC^GCC
SmaI 486 CCCGGG CCC^GGG
Sse8387I 1419 CCTGCAGG CCTGCA^GG
SspBI 649 TGTACA T^GTACA
SspI 1235 AATATT AAT^ATT
StuI 3139 AGGCCT AGG^CCT
Van91I 2723 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 115 CCATTCCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 486 CCCGGG C^CCGGG
XmaI 486 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 218 TCCGGA T^CCGGA
833 TCCGGA
AflII 2682 CTTAAG C^TTAAG
4123 CTTAAG
AflIII 1212 ACGTGT A^C[GA][CT]GT
2130 ACGTGT
AocI 99 CCTCAGG CC^T[ACGT]AGG
2442 CCTCAGG
AvrII 2256 CCTAGG C^CTAGG
2395 CCTAGG
BciVI 327 GGATAC GTATCC(6/5)
845 GGATAC
BfrI 2682 CTTAAG C^TTAAG
4123 CTTAAG
BglII 2463 AGATCT A^GATCT
2650 AGATCT
BlnI 2256 CCTAGG C^CTAGG
2395 CCTAGG
BsaBI 915 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3655 GATAATGATC
BsaI 2400 GGTCTC GGTCTC(1/5)
2517 GGTCTC
BseAI 218 TCCGGA T^CCGGA
833 TCCGGA
BsgI 3627 CTGCAC GTGCAG(16/14)
840 GTGCAG
BspEI 218 TCCGGA T^CCGGA
833 TCCGGA
BspMI 475 ACCTGC ACCTGC(4/8)
663 ACCTGC
BstEII 2268 GGTGACC G^GT[ACGT]ACC
3560 GGTTACC
BstPI 2268 GGTGACC G^GT[ACGT]ACC
3560 GGTTACC
Bsu36I 99 CCTCAGG CC^T[ACGT]AGG
2442 CCTCAGG
Eco81I 99 CCTCAGG CC^T[ACGT]AGG
2442 CCTCAGG
EcoO65I 2268 GGTGACC G^GT[ACGT]ACC
3560 GGTTACC
HaeII 223 AGCGCC [GA]GCGC^[CT]
498 GGCGCC
HindIII 2955 AAGCTT A^AGCTT
3059 AAGCTT
MamI 915 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3655 GATAATGATC
MroI 218 TCCGGA T^CCGGA
833 TCCGGA
MslI 1004 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2606 CACTTTGATG
SapI 950 GCTCTTC GCTCTTC(1/4)
3617 GCTCTTC
SphI 273 GCATGC GCATG^C
1957 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AspI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII XmnI