##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp700I AspI AvrII BamHI BbrPI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrFI
BsrGI BssHII Bst1107I BstZ17I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EcoO109I FbaI FseI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI PvuI RcaI RsrII SbfI
SfiI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 4247 GGTACC G^GTACC
AclI 3607 AACGTT AA^CGTT
Aor51HI 1923 AGCGCT AGC^GCT
Asp718I 4247 GGTACC G^GTACC
AvaII 769 GGTCC G^G[AT]CC
AviII 2507 TGCGCA TGC^GCA
BalI 642 TGGCCA TGG^CCA
BbeI 2564 GGCGCC GGCGC^C
BbvCI 2610 GCTGAGG CCTCAGC(-5/-2)
BciVI 3568 GGATAC GTATCC(6/5)
BsmBI 3902 GAGACG CGTCTC(1/5)
BspMI 2277 ACCTGC ACCTGC(4/8)
BsrBI 1993 CCGCTC CCGCTC(-3/-3)
BstBI 175 TTCGAA TT^CGAA
BstEII 4117 GGTGACC G^GT[ACGT]ACC
BstPI 4117 GGTGACC G^GT[ACGT]ACC
BstXI 1755 CCAACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 45 GGATCT [GA]^GATC[CT]
Eco47III 1923 AGCGCT AGC^GCT
EcoO65I 4117 GGTGACC G^GT[ACGT]ACC
EcoRV 4093 GATATC GAT^ATC
EcoT22I 833 ATGCAT ATGCA^T
FspI 2507 TGCGCA TGC^GCA
HpaI 3757 GTTAAC GTT^AAC
KasI 2564 GGCGCC G^GCGCC
KpnI 4247 GGTACC GGTAC^C
KspI 2033 CCGCGG CCGC^GG
MflI 45 GGATCT [GA]^GATC[CT]
MluNI 642 TGGCCA TGG^CCA
MscI 642 TGGCCA TGG^CCA
NarI 2564 GGCGCC GG^CGCC
NcoI 866 CCATGG C^CATGG
NotI 2029 GCGGCCGC GC^GGCCGC
NruI 35 TCGCGA TCG^CGA
NsiI 833 ATGCAT ATGCA^T
NspV 175 TTCGAA TT^CGAA
PshAI 1415 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3607 AACGTT AA^CGTT
PstI 2836 CTGCAG CTGCA^G
SacI 3050 GAGCTC GAGCT^C
SacII 2033 CCGCGG CCGC^GG
SalI 1407 GTCGAC G^TCGAC
ScaI 819 AGTACT AGT^ACT
SexAI 1172 ACCAGGT A^CC[AT]GGT
SfoI 2564 GGCGCC GGC^GCC
SfuI 175 TTCGAA TT^CGAA
SphI 3769 GCATGC GCATG^C
SspI 3439 AATATT AAT^ATT
StuI 4317 AGGCCT AGG^CCT
XhoII 45 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 515 GTCTAC GT^[AC][GT]AC
1407 GTCGAC
AccII 36 CGCG CG^CG
2034 CGCG
AflIII 3299 ACATGT A^C[GA][CT]GT
3815 ACATGT
AhdI 415 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3625 GACAATGTGTC
AspEI 415 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3625 GACAATGTGTC
BanI 2564 GGCGCC G^G[CT][GA]CC
4247 GGTACC
BanII 1901 GAGCCC G[GA]GC[CT]^C
3050 GAGCTC
BcgI 2271 CGAGAAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3917 GCATTGACCTCG
BsiEI 2030 CGGCCG CG[GA][CT]^CG
2697 CGGCCG
BsmI 2639 GAATGC GAATGC(1/-1)
537 GCATTC
BspLU11I 3299 ACATGT A^CATGT
3815 ACATGT
BsrDI 1979 GCAATG GCAATG(2/0)
2689 GCAATG
BssSI 1887 CACGAG CACGAG(-5/-1)
650 CTCGTG
BstUI 36 CGCG CG^CG
2034 CGCG
Cfr13I 769 GGTCC G^G[ACGT]CC
3660 GGCCC
EagI 2030 CGGCCG C^GGCCG
2697 CGGCCG
Eam1105I 415 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3625 GACAATGTGTC
EclXI 2030 CGGCCG C^GGCCG
2697 CGGCCG
Eco52I 2030 CGGCCG C^GGCCG
2697 CGGCCG
EcoNI 772 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1629 CCTGAAGAAGG
EcoRI 686 GAATTC G^AATTC
2797 GAATTC
HaeII 1923 AGCGCT [GA]GCGC^[CT]
2564 GGCGCC
HincII 1407 GTCGAC GT[CT]^[GA]AC
3757 GTTAAC
HindII 1407 GTCGAC GT[CT]^[GA]AC
3757 GTTAAC
MvnI 36 CGCG CG^CG
2034 CGCG
PaeR7I 1044 CTCGAG C^TCGAG
3064 CTCGAG
PvuII 443 CAGCTG CAG^CTG
3536 CAGCTG
SapI 753 GAAGAGC GCTCTTC(1/4)
1623 GAAGAGC
Sau96I 769 GGTCC G^G[ACGT]CC
3660 GGCCC
SmaI 138 CCCGGG CCC^GGG
3345 CCCGGG
XhoI 1044 CTCGAG C^TCGAG
3064 CTCGAG
XmaCI 138 CCCGGG C^CCGGG
3345 CCCGGG
XmaI 138 CCCGGG C^CCGGG
3345 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Bsu36I Cac8I CelII
CfoI DdeI DpnII DsaI EaeI
EarI Eco81I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI Sau3AI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI