################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AccIII AclI AflII AocI AscI AseI AsnI AspI AvaI AviII AvrII BbeI BbvCI BciVI BfrI BlnI BseAI BsiWI BsoBI BspEI BssHII BssSI BstBI BstEII BstPI Bsu36I CpoI DraI DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 3050 GGTACC G^GTACC AcsI 3174 GAATTC [GA]^AATT[CT] AgeI 3397 ACCGGT A^CCGGT AhdI 2388 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 43 GGGCCC GGGCC^C ApoI 3174 GAATTC [GA]^AATT[CT] Asp718I 3050 GGTACC G^GTACC AspEI 2388 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2980 GGATCC G^GATCC BclI 1921 TGATCA T^GATCA BsaWI 3397 ACCGGT [AT]^CCGG[AT] BsiEI 2516 CGACCG CG[GA][CT]^CG Bsp1407I 2200 TGTACA T^GTACA BspHI 2009 TCATGA T^CATGA BsrFI 3397 ACCGGT [GA]^CCGG[CT] BsrGI 2200 TGTACA T^GTACA Cfr10I 3397 ACCGGT [GA]^CCGG[CT] Eam1105I 2388 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3174 GAATTC G^AATTC FbaI 1921 TGATCA T^GATCA HincII 80 GTTGAC GT[CT]^[GA]AC HindII 80 GTTGAC GT[CT]^[GA]AC KpnI 3050 GGTACC GGTAC^C PinAI 3397 ACCGGT A^CCGGT PshAI 998 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2009 TCATGA T^CATGA ScaI 3404 AGTACT AGT^ACT SexAI 3166 ACCTGGT A^CC[AT]GGT SfiI 2153 GGCCGACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 2939 GCATGC GCATG^C SspBI 2200 TGTACA T^GTACA SspI 3469 AATATT AAT^ATT XbaI 1850 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AatII 1002 GACGTC GACGT^C 2478 GACGTC AccII 2495 CGCG CG^CG 2699 CGCG AcyI 1002 GACGTC G[GA]^CG[CT]C 2478 GACGTC Alw44I 1644 GTGCAC G^TGCAC 3928 GTGCAC ApaLI 1644 GTGCAC G^TGCAC 3928 GTGCAC BalI 520 TGGCCA TGG^CCA 1837 TGGCCA BbrPI 473 CACGTG CAC^GTG 1499 CACGTG BbsI 1140 GAAGAC GAAGAC(2/6) 1710 GTCTTC BcgI 1147 CGACAGACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1525 CGAAGGAACTGC BglII 1387 AGATCT A^GATCT 1693 AGATCT BpuAI 1140 GAAGAC GAAGAC(2/6) 1710 GTCTTC BsaHI 1002 GACGTC G[GA]^CG[CT]C 2478 GACGTC BsmI 1550 GAATGC GAATGC(1/-1) 325 GCATTC BspDI 1087 ATCGAT AT^CGAT 3222 ATCGAT BspLU11I 2062 ACATGT A^CATGT 2263 ACATGT BsrDI 2276 CATTGC GCAATG(2/0) 3461 GCAATG Bst1107I 538 GTATAC GTA^TAC 2066 GTATAC BstUI 2495 CGCG CG^CG 2699 CGCG BstZ17I 538 GTATAC GTA^TAC 2066 GTATAC ClaI 1087 ATCGAT AT^CGAT 3222 ATCGAT Hin1I 1002 GACGTC G[GA]^CG[CT]C 2478 GACGTC MluNI 520 TGGCCA TGG^CCA 1837 TGGCCA MscI 520 TGGCCA TGG^CCA 1837 TGGCCA MvnI 2495 CGCG CG^CG 2699 CGCG PmaCI 473 CACGTG CAC^GTG 1499 CACGTG PmlI 473 CACGTG CAC^GTG 1499 CACGTG PpuMI 1900 AGGACCT [GA]G^G[AT]CC[CT] 2386 GGGACCT SmlI 2677 CTCAAG C^T[CT][GA]AG 3272 CTCAAG TfiI 149 GATTC G^A[AT]TC 1299 GAATC Tsp45I 409 GTGAC ^GT[GC]AC 1812 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AciI AfaI AflIII AluI AlwI AlwNI Aor51HI Asp700I AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI