##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccIII AclI AflII AocI AscI
AseI AsnI AspI AvaI AviII
AvrII BbeI BbvCI BciVI BfrI
BlnI BseAI BsiWI BsoBI BspEI
BssHII BssSI BstBI BstEII BstPI
Bsu36I CpoI DraI DrdI EagI
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3050 GGTACC G^GTACC
AcsI 3174 GAATTC [GA]^AATT[CT]
AgeI 3397 ACCGGT A^CCGGT
AhdI 2388 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 43 GGGCCC GGGCC^C
ApoI 3174 GAATTC [GA]^AATT[CT]
Asp718I 3050 GGTACC G^GTACC
AspEI 2388 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2980 GGATCC G^GATCC
BclI 1921 TGATCA T^GATCA
BsaWI 3397 ACCGGT [AT]^CCGG[AT]
BsiEI 2516 CGACCG CG[GA][CT]^CG
Bsp1407I 2200 TGTACA T^GTACA
BspHI 2009 TCATGA T^CATGA
BsrFI 3397 ACCGGT [GA]^CCGG[CT]
BsrGI 2200 TGTACA T^GTACA
Cfr10I 3397 ACCGGT [GA]^CCGG[CT]
Eam1105I 2388 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3174 GAATTC G^AATTC
FbaI 1921 TGATCA T^GATCA
HincII 80 GTTGAC GT[CT]^[GA]AC
HindII 80 GTTGAC GT[CT]^[GA]AC
KpnI 3050 GGTACC GGTAC^C
PinAI 3397 ACCGGT A^CCGGT
PshAI 998 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2009 TCATGA T^CATGA
ScaI 3404 AGTACT AGT^ACT
SexAI 3166 ACCTGGT A^CC[AT]GGT
SfiI 2153 GGCCGACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2939 GCATGC GCATG^C
SspBI 2200 TGTACA T^GTACA
SspI 3469 AATATT AAT^ATT
XbaI 1850 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 1002 GACGTC GACGT^C
2478 GACGTC
AccII 2495 CGCG CG^CG
2699 CGCG
AcyI 1002 GACGTC G[GA]^CG[CT]C
2478 GACGTC
Alw44I 1644 GTGCAC G^TGCAC
3928 GTGCAC
ApaLI 1644 GTGCAC G^TGCAC
3928 GTGCAC
BalI 520 TGGCCA TGG^CCA
1837 TGGCCA
BbrPI 473 CACGTG CAC^GTG
1499 CACGTG
BbsI 1140 GAAGAC GAAGAC(2/6)
1710 GTCTTC
BcgI 1147 CGACAGACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1525 CGAAGGAACTGC
BglII 1387 AGATCT A^GATCT
1693 AGATCT
BpuAI 1140 GAAGAC GAAGAC(2/6)
1710 GTCTTC
BsaHI 1002 GACGTC G[GA]^CG[CT]C
2478 GACGTC
BsmI 1550 GAATGC GAATGC(1/-1)
325 GCATTC
BspDI 1087 ATCGAT AT^CGAT
3222 ATCGAT
BspLU11I 2062 ACATGT A^CATGT
2263 ACATGT
BsrDI 2276 CATTGC GCAATG(2/0)
3461 GCAATG
Bst1107I 538 GTATAC GTA^TAC
2066 GTATAC
BstUI 2495 CGCG CG^CG
2699 CGCG
BstZ17I 538 GTATAC GTA^TAC
2066 GTATAC
ClaI 1087 ATCGAT AT^CGAT
3222 ATCGAT
Hin1I 1002 GACGTC G[GA]^CG[CT]C
2478 GACGTC
MluNI 520 TGGCCA TGG^CCA
1837 TGGCCA
MscI 520 TGGCCA TGG^CCA
1837 TGGCCA
MvnI 2495 CGCG CG^CG
2699 CGCG
PmaCI 473 CACGTG CAC^GTG
1499 CACGTG
PmlI 473 CACGTG CAC^GTG
1499 CACGTG
PpuMI 1900 AGGACCT [GA]G^G[AT]CC[CT]
2386 GGGACCT
SmlI 2677 CTCAAG C^T[CT][GA]AG
3272 CTCAAG
TfiI 149 GATTC G^A[AT]TC
1299 GAATC
Tsp45I 409 GTGAC ^GT[GC]AC
1812 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AfaI AflIII AluI
AlwI AlwNI Aor51HI Asp700I AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI