##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BglI
BlnI BlpI Bpu1102I BsaI BseAI
BsiWI BspDI BspEI BspHI BssHII
Bst1107I BstZ17I CelII ClaI CpoI
DraIII Eam1105I Eco47III EcoNI EcoRV
FbaI FseI FspI HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI PacI PflFI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SpeI SphI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3169 GACGTC GACGT^C
AgeI 1462 ACCGGT A^CCGGT
Alw44I 3985 GTGCAC G^TGCAC
ApaLI 3985 GTGCAC G^TGCAC
BamHI 1919 GGATCC G^GATCC
BanI 2920 GGCACC G^G[CT][GA]CC
BsaAI 1436 TACGTA [CT]AC^GT[GA]
BsaBI 3960 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 3040 ACCTGC ACCTGC(4/8)
BstBI 1050 TTCGAA TT^CGAA
BstEII 944 GGTCACC G^GT[ACGT]ACC
BstPI 944 GGTCACC G^GT[ACGT]ACC
DrdI 936 GACATTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 944 GGTCACC G^GT[ACGT]ACC
EcoRI 543 GAATTC G^AATTC
EcoT22I 455 ATGCAT ATGCA^T
HindIII 3012 AAGCTT A^AGCTT
MamI 3960 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 455 ATGCAT ATGCA^T
NspV 1050 TTCGAA TT^CGAA
PaeR7I 2987 CTCGAG C^TCGAG
PflMI 3106 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1462 ACCGGT A^CCGGT
PpuMI 1731 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2946 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2147 GCTCTTC GCTCTTC(1/4)
SexAI 3936 ACCTGGT A^CC[AT]GGT
SfcI 228 CTACAG C^T[GA][CT]AG
SfuI 1050 TTCGAA TT^CGAA
SnaBI 1436 TACGTA TAC^GTA
Van91I 3106 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2610 TCTAGA T^CTAGA
XhoI 2987 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 226 GTCTAC GT^[AC][GT]AC
2909 GTCTAC
AccII 1096 CGCG CG^CG
1802 CGCG
AflII 474 CTTAAG C^TTAAG
2307 CTTAAG
AlwNI 2365 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3056 CAGAGACTG
Asp700I 233 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
483 GAAAACCTTC
BalI 701 TGGCCA TGG^CCA
1601 TGGCCA
BbvCI 1561 CCTCAGC CCTCAGC(-5/-2)
2081 GCTGAGG
BcnI 49 CCCGG CC^[GC]GG
3082 CCCGG
BfrI 474 CTTAAG C^TTAAG
2307 CTTAAG
BglII 2613 AGATCT A^GATCT
3965 AGATCT
BsaWI 1462 ACCGGT [AT]^CCGG[AT]
3319 ACCGGA
BsgI 3649 CTGCAC GTGCAG(16/14)
1666 GTGCAG
BsmI 3954 GCATTC GAATGC(1/-1)
3971 GCATTC
Bsp1407I 434 TGTACA T^GTACA
2520 TGTACA
BsrBI 2021 CCGCTC CCGCTC(-3/-3)
1791 GAGCGG
BsrGI 434 TGTACA T^GTACA
2520 TGTACA
BstAPI 3336 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3954 GCATTCGATGC
BstUI 1096 CGCG CG^CG
1802 CGCG
DraII 1731 AGGACCC [GA]G^G[ACGT]CC[CT]
3640 AGGCCCC
EagI 1098 CGGCCG C^GGCCG
3084 CGGCCG
EclXI 1098 CGGCCG C^GGCCG
3084 CGGCCG
Eco52I 1098 CGGCCG C^GGCCG
3084 CGGCCG
EcoO109I 1731 AGGACCC [GA]G^G[ACGT]CC[CT]
3640 AGGCCCC
HaeII 1186 GGCGCT [GA]GCGC^[CT]
2982 AGCGCC
MluNI 701 TGGCCA TGG^CCA
1601 TGGCCA
MscI 701 TGGCCA TGG^CCA
1601 TGGCCA
MvnI 1096 CGCG CG^CG
1802 CGCG
NaeI 1203 GCCGGC GCC^GGC
3086 GCCGGC
NciI 49 CCCGG CC^[GC]GG
3082 CCCGG
NgoMIV 1203 GCCGGC G^CCGGC
3086 GCCGGC
SacI 1751 GAGCTC GAGCT^C
2057 GAGCTC
SspBI 434 TGTACA T^GTACA
2520 TGTACA
SspI 510 AATATT AAT^ATT
3420 AATATT
XmnI 233 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
483 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AocI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspLU11I BsrDI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI Eco81I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII