################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## Acc65I AccIII AclI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BglI BlnI BlpI Bpu1102I BsaI BseAI BsiWI BspDI BspEI BspHI BssHII Bst1107I BstZ17I CelII ClaI CpoI DraIII Eam1105I Eco47III EcoNI EcoRV FbaI FseI FspI HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI PacI PflFI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SpeI SphI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 3169 GACGTC GACGT^C AgeI 1462 ACCGGT A^CCGGT Alw44I 3985 GTGCAC G^TGCAC ApaLI 3985 GTGCAC G^TGCAC BamHI 1919 GGATCC G^GATCC BanI 2920 GGCACC G^G[CT][GA]CC BsaAI 1436 TACGTA [CT]AC^GT[GA] BsaBI 3960 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspMI 3040 ACCTGC ACCTGC(4/8) BstBI 1050 TTCGAA TT^CGAA BstEII 944 GGTCACC G^GT[ACGT]ACC BstPI 944 GGTCACC G^GT[ACGT]ACC DrdI 936 GACATTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 944 GGTCACC G^GT[ACGT]ACC EcoRI 543 GAATTC G^AATTC EcoT22I 455 ATGCAT ATGCA^T HindIII 3012 AAGCTT A^AGCTT MamI 3960 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NsiI 455 ATGCAT ATGCA^T NspV 1050 TTCGAA TT^CGAA PaeR7I 2987 CTCGAG C^TCGAG PflMI 3106 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1462 ACCGGT A^CCGGT PpuMI 1731 AGGACCC [GA]G^G[AT]CC[CT] PshAI 2946 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2147 GCTCTTC GCTCTTC(1/4) SexAI 3936 ACCTGGT A^CC[AT]GGT SfcI 228 CTACAG C^T[GA][CT]AG SfuI 1050 TTCGAA TT^CGAA SnaBI 1436 TACGTA TAC^GTA Van91I 3106 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2610 TCTAGA T^CTAGA XhoI 2987 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 226 GTCTAC GT^[AC][GT]AC 2909 GTCTAC AccII 1096 CGCG CG^CG 1802 CGCG AflII 474 CTTAAG C^TTAAG 2307 CTTAAG AlwNI 2365 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 3056 CAGAGACTG Asp700I 233 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 483 GAAAACCTTC BalI 701 TGGCCA TGG^CCA 1601 TGGCCA BbvCI 1561 CCTCAGC CCTCAGC(-5/-2) 2081 GCTGAGG BcnI 49 CCCGG CC^[GC]GG 3082 CCCGG BfrI 474 CTTAAG C^TTAAG 2307 CTTAAG BglII 2613 AGATCT A^GATCT 3965 AGATCT BsaWI 1462 ACCGGT [AT]^CCGG[AT] 3319 ACCGGA BsgI 3649 CTGCAC GTGCAG(16/14) 1666 GTGCAG BsmI 3954 GCATTC GAATGC(1/-1) 3971 GCATTC Bsp1407I 434 TGTACA T^GTACA 2520 TGTACA BsrBI 2021 CCGCTC CCGCTC(-3/-3) 1791 GAGCGG BsrGI 434 TGTACA T^GTACA 2520 TGTACA BstAPI 3336 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3954 GCATTCGATGC BstUI 1096 CGCG CG^CG 1802 CGCG DraII 1731 AGGACCC [GA]G^G[ACGT]CC[CT] 3640 AGGCCCC EagI 1098 CGGCCG C^GGCCG 3084 CGGCCG EclXI 1098 CGGCCG C^GGCCG 3084 CGGCCG Eco52I 1098 CGGCCG C^GGCCG 3084 CGGCCG EcoO109I 1731 AGGACCC [GA]G^G[ACGT]CC[CT] 3640 AGGCCCC HaeII 1186 GGCGCT [GA]GCGC^[CT] 2982 AGCGCC MluNI 701 TGGCCA TGG^CCA 1601 TGGCCA MscI 701 TGGCCA TGG^CCA 1601 TGGCCA MvnI 1096 CGCG CG^CG 1802 CGCG NaeI 1203 GCCGGC GCC^GGC 3086 GCCGGC NciI 49 CCCGG CC^[GC]GG 3082 CCCGG NgoMIV 1203 GCCGGC G^CCGGC 3086 GCCGGC SacI 1751 GAGCTC GAGCT^C 2057 GAGCTC SspBI 434 TGTACA T^GTACA 2520 TGTACA SspI 510 AATATT AAT^ATT 3420 AATATT XmnI 233 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 483 GAAAACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AocI ApoI AspHI AvaI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspLU11I BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DsaI EaeI EarI Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII