################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AscI Asp718I BbeI BciVI BfrI BglI BsaI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I FseI HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NspI NspV PacI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 948 GGCGTC G[GA]^CG[CT]C AgeI 609 ACCGGT A^CCGGT AhdI 4379 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2236 GTGCAC G^TGCAC AocI 191 CCTCAGG CC^T[ACGT]AGG ApaLI 2236 GTGCAC G^TGCAC AseI 806 ATTAAT AT^TAAT AsnI 806 ATTAAT AT^TAAT AspEI 4379 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3664 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2857 CCTAGG C^CTAGG BbrPI 3659 CACGTG CAC^GTG BbvCI 3926 GCTGAGG CCTCAGC(-5/-2) BlnI 2857 CCTAGG C^CTAGG BsaAI 3659 CACGTG [CT]AC^GT[GA] BsaHI 948 GGCGTC G[GA]^CG[CT]C BsaWI 609 ACCGGT [AT]^CCGG[AT] BsiEI 611 CGGTCG CG[GA][CT]^CG BsmBI 264 GAGACG CGTCTC(1/5) BspHI 4420 TCATGA T^CATGA BsrFI 609 ACCGGT [GA]^CCGG[CT] BstEII 388 GGTGACC G^GT[ACGT]ACC BstPI 388 GGTGACC G^GT[ACGT]ACC Bsu36I 191 CCTCAGG CC^T[ACGT]AGG Cfr10I 609 ACCGGT [GA]^CCGG[CT] Eam1105I 4379 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 191 CCTCAGG CC^T[ACGT]AGG EcoNI 4153 CCTCCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 388 GGTGACC G^GT[ACGT]ACC EcoRV 689 GATATC GAT^ATC EcoT22I 1615 ATGCAT ATGCA^T FauI 1283 GCGGG CCCGC(4/6) Hin1I 948 GGCGTC G[GA]^CG[CT]C NsiI 1615 ATGCAT ATGCA^T PaeR7I 39 CTCGAG C^TCGAG PflFI 3664 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 904 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 609 ACCGGT A^CCGGT PmaCI 3659 CACGTG CAC^GTG PmlI 3659 CACGTG CAC^GTG PpuMI 2870 AGGTCCC [GA]G^G[AT]CC[CT] PshBI 806 ATTAAT AT^TAAT PvuII 1310 CAGCTG CAG^CTG RcaI 4420 TCATGA T^CATGA Tth111I 3664 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 904 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 550 TCTAGA T^CTAGA XhoI 39 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 478 CGCG CG^CG 2133 CGCG Aor51HI 23 AGCGCT AGC^GCT 3923 AGCGCT ApaI 1514 GGGCCC GGGCC^C 1674 GGGCCC AvaI 39 CTCGAG C^[CT]CG[GA]G 1517 CCCGAG AviII 582 TGCGCA TGC^GCA 2026 TGCGCA BamHI 729 GGATCC G^GATCC 1577 GGATCC BanI 3223 GGCACC G^G[CT][GA]CC 3692 GGCACC BglII 1651 AGATCT A^GATCT 2296 AGATCT BlpI 1339 GCTCAGC GC^T[ACGT]AGC 3181 GCTGAGC Bpu1102I 1339 GCTCAGC GC^T[ACGT]AGC 3181 GCTGAGC BsaBI 2291 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3823 GATCTTTATC BsoBI 39 CTCGAG C^[CT]CG[GA]G 1517 CCCGAG BstUI 478 CGCG CG^CG 2133 CGCG CelII 1339 GCTCAGC GC^T[ACGT]AGC 3181 GCTGAGC DraI 707 TTTAAA TTT^AAA 4276 TTTAAA DraIII 1386 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 4407 CACATGGTG Eco47III 23 AGCGCT AGC^GCT 3923 AGCGCT FspI 582 TGCGCA TGC^GCA 2026 TGCGCA HaeII 23 AGCGCT [GA]GCGC^[CT] 3923 AGCGCT HincII 923 GTCAAC GT[CT]^[GA]AC 2814 GTCAAC HindII 923 GTCAAC GT[CT]^[GA]AC 2814 GTCAAC HindIII 3229 AAGCTT A^AGCTT 4248 AAGCTT MamI 2291 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3823 GATCTTTATC MspA1I 1310 CAGCTG C[AC]G^C[GT]G 3010 CAGCGG MvnI 478 CGCG CG^CG 2133 CGCG NdeI 156 CATATG CA^TATG 3242 CATATG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanII BbsI BbvI BcgI BclI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI