##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AscI Asp718I BbeI BciVI
BfrI BglI BsaI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I FseI HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspI NspV
PacI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 948 GGCGTC G[GA]^CG[CT]C
AgeI 609 ACCGGT A^CCGGT
AhdI 4379 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2236 GTGCAC G^TGCAC
AocI 191 CCTCAGG CC^T[ACGT]AGG
ApaLI 2236 GTGCAC G^TGCAC
AseI 806 ATTAAT AT^TAAT
AsnI 806 ATTAAT AT^TAAT
AspEI 4379 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3664 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2857 CCTAGG C^CTAGG
BbrPI 3659 CACGTG CAC^GTG
BbvCI 3926 GCTGAGG CCTCAGC(-5/-2)
BlnI 2857 CCTAGG C^CTAGG
BsaAI 3659 CACGTG [CT]AC^GT[GA]
BsaHI 948 GGCGTC G[GA]^CG[CT]C
BsaWI 609 ACCGGT [AT]^CCGG[AT]
BsiEI 611 CGGTCG CG[GA][CT]^CG
BsmBI 264 GAGACG CGTCTC(1/5)
BspHI 4420 TCATGA T^CATGA
BsrFI 609 ACCGGT [GA]^CCGG[CT]
BstEII 388 GGTGACC G^GT[ACGT]ACC
BstPI 388 GGTGACC G^GT[ACGT]ACC
Bsu36I 191 CCTCAGG CC^T[ACGT]AGG
Cfr10I 609 ACCGGT [GA]^CCGG[CT]
Eam1105I 4379 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 191 CCTCAGG CC^T[ACGT]AGG
EcoNI 4153 CCTCCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 388 GGTGACC G^GT[ACGT]ACC
EcoRV 689 GATATC GAT^ATC
EcoT22I 1615 ATGCAT ATGCA^T
FauI 1283 GCGGG CCCGC(4/6)
Hin1I 948 GGCGTC G[GA]^CG[CT]C
NsiI 1615 ATGCAT ATGCA^T
PaeR7I 39 CTCGAG C^TCGAG
PflFI 3664 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 904 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 609 ACCGGT A^CCGGT
PmaCI 3659 CACGTG CAC^GTG
PmlI 3659 CACGTG CAC^GTG
PpuMI 2870 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 806 ATTAAT AT^TAAT
PvuII 1310 CAGCTG CAG^CTG
RcaI 4420 TCATGA T^CATGA
Tth111I 3664 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 904 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 550 TCTAGA T^CTAGA
XhoI 39 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 478 CGCG CG^CG
2133 CGCG
Aor51HI 23 AGCGCT AGC^GCT
3923 AGCGCT
ApaI 1514 GGGCCC GGGCC^C
1674 GGGCCC
AvaI 39 CTCGAG C^[CT]CG[GA]G
1517 CCCGAG
AviII 582 TGCGCA TGC^GCA
2026 TGCGCA
BamHI 729 GGATCC G^GATCC
1577 GGATCC
BanI 3223 GGCACC G^G[CT][GA]CC
3692 GGCACC
BglII 1651 AGATCT A^GATCT
2296 AGATCT
BlpI 1339 GCTCAGC GC^T[ACGT]AGC
3181 GCTGAGC
Bpu1102I 1339 GCTCAGC GC^T[ACGT]AGC
3181 GCTGAGC
BsaBI 2291 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3823 GATCTTTATC
BsoBI 39 CTCGAG C^[CT]CG[GA]G
1517 CCCGAG
BstUI 478 CGCG CG^CG
2133 CGCG
CelII 1339 GCTCAGC GC^T[ACGT]AGC
3181 GCTGAGC
DraI 707 TTTAAA TTT^AAA
4276 TTTAAA
DraIII 1386 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4407 CACATGGTG
Eco47III 23 AGCGCT AGC^GCT
3923 AGCGCT
FspI 582 TGCGCA TGC^GCA
2026 TGCGCA
HaeII 23 AGCGCT [GA]GCGC^[CT]
3923 AGCGCT
HincII 923 GTCAAC GT[CT]^[GA]AC
2814 GTCAAC
HindII 923 GTCAAC GT[CT]^[GA]AC
2814 GTCAAC
HindIII 3229 AAGCTT A^AGCTT
4248 AAGCTT
MamI 2291 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3823 GATCTTTATC
MspA1I 1310 CAGCTG C[AC]G^C[GT]G
3010 CAGCGG
MvnI 478 CGCG CG^CG
2133 CGCG
NdeI 156 CATATG CA^TATG
3242 CATATG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanII BbsI BbvI
BcgI BclI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI