##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BalI BclI BfrI BlnI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspHI BsrDI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
Eam1105I EcoO65I EcoRI EcoT22I FauI
FbaI FseI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SacII SbfI ScaI SfiI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AocI 3257 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1330 AGCGCT AGC^GCT
ApaI 2985 GGGCCC GGGCC^C
AspI 4026 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1980 GGCGCC GGCGC^C
BcgI 774 CGAGATCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 933 GGATAC GTATCC(6/5)
BglII 164 AGATCT A^GATCT
BsaBI 1995 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 2558 TGTACA T^GTACA
BsrGI 2558 TGTACA T^GTACA
BstBI 838 TTCGAA TT^CGAA
Bsu36I 3257 CCTGAGG CC^T[ACGT]AGG
DraI 4378 TTTAAA TTT^AAA
DrdI 1930 GACGTGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3704 CGGCCG C^GGCCG
EclXI 3704 CGGCCG C^GGCCG
Eco47III 1330 AGCGCT AGC^GCT
Eco52I 3704 CGGCCG C^GGCCG
Eco81I 3257 CCTGAGG CC^T[ACGT]AGG
EcoRV 2871 GATATC GAT^ATC
HindIII 463 AAGCTT A^AGCTT
HpaI 444 GTTAAC GTT^AAC
KasI 1980 GGCGCC G^GCGCC
MamI 1995 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1980 GGCGCC GG^CGCC
NspV 838 TTCGAA TT^CGAA
PflFI 4026 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3430 CGATCG CGAT^CG
SalI 1527 GTCGAC G^TCGAC
SfoI 1980 GGCGCC GGC^GCC
SfuI 838 TTCGAA TT^CGAA
SmaI 3972 CCCGGG CCC^GGG
SspBI 2558 TGTACA T^GTACA
Tth111I 4026 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3972 CCCGGG C^CCGGG
XmaI 3972 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1384 GTAGAC GT^[AC][GT]AC
1527 GTCGAC
AcyI 1980 GGCGCC G[GA]^CG[CT]C
3253 GACGCC
Alw44I 1798 GTGCAC G^TGCAC
2029 GTGCAC
ApaLI 1798 GTGCAC G^TGCAC
2029 GTGCAC
AviII 552 TGCGCA TGC^GCA
2591 TGCGCA
BbsI 2581 GTCTTC GAAGAC(2/6)
2794 GTCTTC
BlpI 1025 GCTCAGC GC^T[ACGT]AGC
3322 GCTGAGC
Bpu1102I 1025 GCTCAGC GC^T[ACGT]AGC
3322 GCTGAGC
BpuAI 2581 GTCTTC GAAGAC(2/6)
2794 GTCTTC
BsaHI 1980 GGCGCC G[GA]^CG[CT]C
3253 GACGCC
BsaI 658 GAGACC GGTCTC(1/5)
2846 GAGACC
BsmBI 1136 CGTCTC CGTCTC(1/5)
1928 GAGACG
BspLU11I 734 ACATGT A^CATGT
4078 ACATGT
BstAPI 1238 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4212 GCACCTGCTGC
CelII 1025 GCTCAGC GC^T[ACGT]AGC
3322 GCTGAGC
EcoNI 3223 CCTATGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3682 CCTGACACAGG
FspI 552 TGCGCA TGC^GCA
2591 TGCGCA
HaeII 1330 AGCGCT [GA]GCGC^[CT]
1980 GGCGCC
Hin1I 1980 GGCGCC G[GA]^CG[CT]C
3253 GACGCC
HincII 444 GTTAAC GT[CT]^[GA]AC
1527 GTCGAC
HindII 444 GTTAAC GT[CT]^[GA]AC
1527 GTCGAC
PaeR7I 100 CTCGAG C^TCGAG
187 CTCGAG
PflMI 291 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2597 CCATGGAGTGG
SexAI 2345 ACCTGGT A^CC[AT]GGT
4027 ACCAGGT
SphI 2492 GCATGC GCATG^C
2924 GCATGC
Van91I 291 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2597 CCATGGAGTGG
XcmI 1550 CCAGTCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3415 CCACATTGTTGGTGG
XhoI 100 CTCGAG C^TCGAG
187 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BamHI BanI BanII BbrPI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII