################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AhdI Aor51HI AscI Asp718I AspEI AviII BamHI BbeI BbvCI BcgI BclI BsaBI BseAI BsiEI Bsp1407I BspDI BspEI BspHI BsrGI Bst1107I BstAPI BstZ17I ClaI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI FspI HaeII KasI KpnI MamI MfeI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PmeI Psp1406I PvuI RcaI SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 170 GGCGTC G[GA]^CG[CT]C AgeI 625 ACCGGT A^CCGGT ApaI 3085 GGGCCC GGGCC^C AseI 773 ATTAAT AT^TAAT AsnI 773 ATTAAT AT^TAAT Asp700I 1304 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 3296 CACGTG CAC^GTG BciVI 2018 GTATCC GTATCC(6/5) BglI 3168 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2688 AGATCT A^GATCT BlpI 74 GCTGAGC GC^T[ACGT]AGC Bpu1102I 74 GCTGAGC GC^T[ACGT]AGC BsaHI 170 GGCGTC G[GA]^CG[CT]C BsaWI 625 ACCGGT [AT]^CCGG[AT] BsiWI 2338 CGTACG C^GTACG BspLU11I 3478 ACATGT A^CATGT BsrBI 905 GAGCGG CCGCTC(-3/-3) BsrFI 625 ACCGGT [GA]^CCGG[CT] BssHII 65 GCGCGC G^CGCGC BstBI 1311 TTCGAA TT^CGAA BstEII 2552 GGTCACC G^GT[ACGT]ACC BstPI 2552 GGTCACC G^GT[ACGT]ACC BstXI 3824 CCAACATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 74 GCTGAGC GC^T[ACGT]AGC Cfr10I 625 ACCGGT [GA]^CCGG[CT] CpoI 275 CGGACCG CG^G[AT]CCG EcoO65I 2552 GGTCACC G^GT[ACGT]ACC Hin1I 170 GGCGTC G[GA]^CG[CT]C HindIII 4282 AAGCTT A^AGCTT KspI 13 CCGCGG CCGC^GG NspV 1311 TTCGAA TT^CGAA PaeR7I 592 CTCGAG C^TCGAG PflMI 3500 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 625 ACCGGT A^CCGGT PmaCI 3296 CACGTG CAC^GTG PmlI 3296 CACGTG CAC^GTG PshAI 3110 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 773 ATTAAT AT^TAAT RsrII 275 CGGACCG CG^G[AT]CCG SacI 1925 GAGCTC GAGCT^C SacII 13 CCGCGG CCGC^GG SfuI 1311 TTCGAA TT^CGAA SspI 4073 AATATT AAT^ATT Van91I 3500 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 592 CTCGAG C^TCGAG XmnI 1304 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflII 3353 CTTAAG C^TTAAG 3684 CTTAAG Alw44I 1862 GTGCAC G^TGCAC 1886 GTGCAC ApaLI 1862 GTGCAC G^TGCAC 1886 GTGCAC AvrII 2195 CCTAGG C^CTAGG 3316 CCTAGG BfrI 3353 CTTAAG C^TTAAG 3684 CTTAAG BlnI 2195 CCTAGG C^CTAGG 3316 CCTAGG BsaAI 2313 TACGTG [CT]AC^GT[GA] 3296 CACGTG BsaI 3976 GAGACC GGTCTC(1/5) 340 GGTCTC BsmBI 187 CGTCTC CGTCTC(1/5) 754 CGTCTC BsmI 3953 GAATGC GAATGC(1/-1) 2035 GCATTC DrdI 1940 GACGAACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2463 GACTCAACAGTC EcoT22I 2033 ATGCAT ATGCA^T 4160 ATGCAT HpaI 3339 GTTAAC GTT^AAC 3745 GTTAAC MluI 267 ACGCGT A^CGCGT 1162 ACGCGT NsiI 2033 ATGCAT ATGCA^T 4160 ATGCAT PvuII 533 CAGCTG CAG^CTG 3573 CAGCTG SapI 552 GAAGAGC GCTCTTC(1/4) 2179 GCTCTTC SmaI 299 CCCGGG CCC^GGG 2087 CCCGGG SphI 1099 GCATGC GCATG^C 4162 GCATGC StuI 3128 AGGCCT AGG^CCT 3319 AGGCCT XmaCI 299 CCCGGG C^CCGGG 2087 CCCGGG XmaI 299 CCCGGG C^CCGGG 2087 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII