##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AhdI Aor51HI AscI Asp718I AspEI
AviII BamHI BbeI BbvCI BcgI
BclI BsaBI BseAI BsiEI Bsp1407I
BspDI BspEI BspHI BsrGI Bst1107I
BstAPI BstZ17I ClaI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HaeII
KasI KpnI MamI MfeI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PmeI Psp1406I PvuI RcaI SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 170 GGCGTC G[GA]^CG[CT]C
AgeI 625 ACCGGT A^CCGGT
ApaI 3085 GGGCCC GGGCC^C
AseI 773 ATTAAT AT^TAAT
AsnI 773 ATTAAT AT^TAAT
Asp700I 1304 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 3296 CACGTG CAC^GTG
BciVI 2018 GTATCC GTATCC(6/5)
BglI 3168 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2688 AGATCT A^GATCT
BlpI 74 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 74 GCTGAGC GC^T[ACGT]AGC
BsaHI 170 GGCGTC G[GA]^CG[CT]C
BsaWI 625 ACCGGT [AT]^CCGG[AT]
BsiWI 2338 CGTACG C^GTACG
BspLU11I 3478 ACATGT A^CATGT
BsrBI 905 GAGCGG CCGCTC(-3/-3)
BsrFI 625 ACCGGT [GA]^CCGG[CT]
BssHII 65 GCGCGC G^CGCGC
BstBI 1311 TTCGAA TT^CGAA
BstEII 2552 GGTCACC G^GT[ACGT]ACC
BstPI 2552 GGTCACC G^GT[ACGT]ACC
BstXI 3824 CCAACATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 74 GCTGAGC GC^T[ACGT]AGC
Cfr10I 625 ACCGGT [GA]^CCGG[CT]
CpoI 275 CGGACCG CG^G[AT]CCG
EcoO65I 2552 GGTCACC G^GT[ACGT]ACC
Hin1I 170 GGCGTC G[GA]^CG[CT]C
HindIII 4282 AAGCTT A^AGCTT
KspI 13 CCGCGG CCGC^GG
NspV 1311 TTCGAA TT^CGAA
PaeR7I 592 CTCGAG C^TCGAG
PflMI 3500 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 625 ACCGGT A^CCGGT
PmaCI 3296 CACGTG CAC^GTG
PmlI 3296 CACGTG CAC^GTG
PshAI 3110 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 773 ATTAAT AT^TAAT
RsrII 275 CGGACCG CG^G[AT]CCG
SacI 1925 GAGCTC GAGCT^C
SacII 13 CCGCGG CCGC^GG
SfuI 1311 TTCGAA TT^CGAA
SspI 4073 AATATT AAT^ATT
Van91I 3500 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 592 CTCGAG C^TCGAG
XmnI 1304 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 3353 CTTAAG C^TTAAG
3684 CTTAAG
Alw44I 1862 GTGCAC G^TGCAC
1886 GTGCAC
ApaLI 1862 GTGCAC G^TGCAC
1886 GTGCAC
AvrII 2195 CCTAGG C^CTAGG
3316 CCTAGG
BfrI 3353 CTTAAG C^TTAAG
3684 CTTAAG
BlnI 2195 CCTAGG C^CTAGG
3316 CCTAGG
BsaAI 2313 TACGTG [CT]AC^GT[GA]
3296 CACGTG
BsaI 3976 GAGACC GGTCTC(1/5)
340 GGTCTC
BsmBI 187 CGTCTC CGTCTC(1/5)
754 CGTCTC
BsmI 3953 GAATGC GAATGC(1/-1)
2035 GCATTC
DrdI 1940 GACGAACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2463 GACTCAACAGTC
EcoT22I 2033 ATGCAT ATGCA^T
4160 ATGCAT
HpaI 3339 GTTAAC GTT^AAC
3745 GTTAAC
MluI 267 ACGCGT A^CGCGT
1162 ACGCGT
NsiI 2033 ATGCAT ATGCA^T
4160 ATGCAT
PvuII 533 CAGCTG CAG^CTG
3573 CAGCTG
SapI 552 GAAGAGC GCTCTTC(1/4)
2179 GCTCTTC
SmaI 299 CCCGGG CCC^GGG
2087 CCCGGG
SphI 1099 GCATGC GCATG^C
4162 GCATGC
StuI 3128 AGGCCT AGG^CCT
3319 AGGCCT
XmaCI 299 CCCGGG C^CCGGG
2087 CCCGGG
XmaI 299 CCCGGG C^CCGGG
2087 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII