##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI AflIII Aor51HI
AscI AseI AsnI AspI AvrII
BalI BcgI BciVI BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BspEI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstBI BstZ17I CelII CpoI Eco47III
EcoNI FseI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NheI NspV PacI PflFI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I StuI
SwaI Tth111I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 3124 GTAGAC GT^[AC][GT]AC
AflII 2827 CTTAAG C^TTAAG
AgeI 605 ACCGGT A^CCGGT
AhdI 3127 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3162 CCTAAGG CC^T[ACGT]AGG
ApaI 2026 GGGCCC GGGCC^C
Asp700I 3401 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3127 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3144 TGCGCA TGC^GCA
BbsI 2067 GAAGAC GAAGAC(2/6)
BfrI 2827 CTTAAG C^TTAAG
BglI 251 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2067 GAAGAC GAAGAC(2/6)
BsiEI 649 CGGCCG CG[GA][CT]^CG
BsmBI 566 GAGACG CGTCTC(1/5)
Bsp1407I 4101 TGTACA T^GTACA
BspDI 1765 ATCGAT AT^CGAT
BsrGI 4101 TGTACA T^GTACA
Bsu36I 3162 CCTAAGG CC^T[ACGT]AGG
ClaI 1765 ATCGAT AT^CGAT
DraIII 3650 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 649 CGGCCG [CT]^GGCC[GA]
EagI 649 CGGCCG C^GGCCG
Eam1105I 3127 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 649 CGGCCG C^GGCCG
Eco52I 649 CGGCCG C^GGCCG
Eco81I 3162 CCTAAGG CC^T[ACGT]AGG
EcoRV 1415 GATATC GAT^ATC
EcoT22I 3729 ATGCAT ATGCA^T
FspI 3144 TGCGCA TGC^GCA
HindIII 1811 AAGCTT A^AGCTT
HpaI 2530 GTTAAC GTT^AAC
NaeI 363 GCCGGC GCC^GGC
NdeI 3732 CATATG CA^TATG
NgoMIV 363 GCCGGC G^CCGGC
NotI 648 GCGGCCGC GC^GGCCGC
NruI 847 TCGCGA TCG^CGA
NsiI 3729 ATGCAT ATGCA^T
PaeR7I 526 CTCGAG C^TCGAG
PinAI 605 ACCGGT A^CCGGT
PshAI 2132 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2575 CTGCAG CTGCA^G
SacI 1927 GAGCTC GAGCT^C
SapI 866 GAAGAGC GCTCTTC(1/4)
SmaI 2030 CCCGGG CCC^GGG
SpeI 3278 ACTAGT A^CTAGT
SphI 3727 GCATGC GCATG^C
SspBI 4101 TGTACA T^GTACA
SspI 4011 AATATT AAT^ATT
XhoI 526 CTCGAG C^TCGAG
XmaCI 2030 CCCGGG C^CCGGG
XmaI 2030 CCCGGG C^CCGGG
XmnI 3401 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 608 GGTACC G^GTACC
2753 GGTACC
Alw44I 2156 GTGCAC G^TGCAC
3738 GTGCAC
AlwNI 1525 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1683 CAGTCTCTG
ApaLI 2156 GTGCAC G^TGCAC
3738 GTGCAC
Asp718I 608 GGTACC G^GTACC
2753 GGTACC
BamHI 48 GGATCC G^GATCC
82 GGATCC
BbrPI 2594 CACGTG CAC^GTG
3650 CACGTG
BclI 1806 TGATCA T^GATCA
2710 TGATCA
BglII 2324 AGATCT A^GATCT
2579 AGATCT
BsaAI 2594 CACGTG [CT]AC^GT[GA]
3650 CACGTG
BseRI 469 CTCCTC GAGGAG(10/8)
1610 GAGGAG
BsmI 536 GCATTC GAATGC(1/-1)
2664 GCATTC
BspHI 1372 TCATGA T^CATGA
2313 TCATGA
BspMI 2660 ACCTGC ACCTGC(4/8)
112 GCAGGT
BssSI 1062 CACGAG CACGAG(-5/-1)
1910 CTCGTG
BstEII 601 GGTCACC G^GT[ACGT]ACC
712 GGTGACC
BstPI 601 GGTCACC G^GT[ACGT]ACC
712 GGTGACC
DraI 2495 TTTAAA TTT^AAA
4090 TTTAAA
DrdI 1213 GACCACGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1676 GACAGTGCAGTC
EcoO65I 601 GGTCACC G^GT[ACGT]ACC
712 GGTGACC
FbaI 1806 TGATCA T^GATCA
2710 TGATCA
KpnI 608 GGTACC GGTAC^C
2753 GGTACC
PflMI 2757 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3649 CCACGTGGTGG
PmaCI 2594 CACGTG CAC^GTG
3650 CACGTG
PmlI 2594 CACGTG CAC^GTG
3650 CACGTG
PvuII 776 CAGCTG CAG^CTG
853 CAGCTG
RcaI 1372 TCATGA T^CATGA
2313 TCATGA
Van91I 2757 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3649 CCACGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII