##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspI
AvrII BamHI BbeI BbrPI BcgI
BclI BglI BglII BlnI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI Eco47III EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI MamI
MfeI MluI MroI MunI NarI
NdeI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2220 GGTACC G^GTACC
AflII 3088 CTTAAG C^TTAAG
AhdI 1312 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3807 CCTGAGG CC^T[ACGT]AGG
ApaI 754 GGGCCC GGGCC^C
Asp700I 1672 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2220 GGTACC G^GTACC
AspEI 1312 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 715 GGATAC GTATCC(6/5)
BfrI 3088 CTTAAG C^TTAAG
BlpI 3765 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3765 GCTGAGC GC^T[ACGT]AGC
BsaAI 1641 TACGTG [CT]AC^GT[GA]
BsiEI 648 CGGCCG CG[GA][CT]^CG
BsrBI 3860 GAGCGG CCGCTC(-3/-3)
BsrDI 2255 CATTGC GCAATG(2/0)
BssSI 2623 CTCGTG CACGAG(-5/-1)
BstYI 3646 AGATCC [GA]^GATC[CT]
Bsu36I 3807 CCTGAGG CC^T[ACGT]AGG
CelII 3765 GCTGAGC GC^T[ACGT]AGC
DraI 2679 TTTAAA TTT^AAA
DraIII 3775 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4177 GACCAAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 648 CGGCCG C^GGCCG
Eam1105I 1312 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 648 CGGCCG C^GGCCG
Eco52I 648 CGGCCG C^GGCCG
Eco81I 3807 CCTGAGG CC^T[ACGT]AGG
EcoRI 1910 GAATTC G^AATTC
KpnI 2220 GGTACC GGTAC^C
KspI 651 CCGCGG CCGC^GG
MflI 3646 AGATCC [GA]^GATC[CT]
NaeI 2235 GCCGGC GCC^GGC
NgoMIV 2235 GCCGGC G^CCGGC
NheI 74 GCTAGC G^CTAGC
NotI 647 GCGGCCGC GC^GGCCGC
NruI 1088 TCGCGA TCG^CGA
PshAI 2880 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 651 CCGCGG CCGC^GG
SapI 1209 GCTCTTC GCTCTTC(1/4)
SmaI 1633 CCCGGG CCC^GGG
SphI 1648 GCATGC GCATG^C
SspI 3536 AATATT AAT^ATT
TfiI 783 GAATC G^A[AT]TC
XbaI 3747 TCTAGA T^CTAGA
XhoII 3646 AGATCC [GA]^GATC[CT]
XmaCI 1633 CCCGGG C^CCGGG
XmaI 1633 CCCGGG C^CCGGG
XmnI 1672 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 216 ACATGT A^C[GA][CT]GT
1070 ACATGT
AvaI 1633 CCCGGG C^[CT]CG[GA]G
3709 CCCGAG
BbvCI 451 CCTCAGC CCTCAGC(-5/-2)
3936 CCTCAGC
BsaWI 179 ACCGGA [AT]^CCGG[AT]
1121 TCCGGT
BsoBI 1633 CCCGGG C^[CT]CG[GA]G
3709 CCCGAG
Bsp1407I 213 TGTACA T^GTACA
1073 TGTACA
BspHI 318 TCATGA T^CATGA
803 TCATGA
BspLU11I 216 ACATGT A^CATGT
1070 ACATGT
BsrGI 213 TGTACA T^GTACA
1073 TGTACA
BstEII 676 GGTGACC G^GT[ACGT]ACC
1182 GGTGACC
BstPI 676 GGTGACC G^GT[ACGT]ACC
1182 GGTGACC
EcoO65I 676 GGTGACC G^GT[ACGT]ACC
1182 GGTGACC
HaeII 3 GGCGCT [GA]GCGC^[CT]
962 AGCGCC
NcoI 354 CCATGG C^CATGG
1237 CCATGG
RcaI 318 TCATGA T^CATGA
803 TCATGA
SspBI 213 TGTACA T^GTACA
1073 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI AspHI AvaII AviII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI