##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BcgI BlnI BsaAI BsaHI
BsaI BsgI BsiWI BsmBI BsmI
BspDI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DrdI Eam1105I Eco47III
EcoO65I EcoRV FseI FspI Hin1I
KasI KpnI KspI MaeII MfeI
MluI MunI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 441 GTAGAC GT^[AC][GT]AC
AccIII 2874 TCCGGA T^CCGGA
AflII 3023 CTTAAG C^TTAAG
AflIII 3216 ACATGT A^C[GA][CT]GT
ApaI 5 GGGCCC GGGCC^C
AseI 3610 ATTAAT AT^TAAT
AsnI 3610 ATTAAT AT^TAAT
BamHI 185 GGATCC G^GATCC
BciVI 2252 GGATAC GTATCC(6/5)
BclI 3140 TGATCA T^GATCA
BfrI 3023 CTTAAG C^TTAAG
BsaBI 2977 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2874 TCCGGA T^CCGGA
Bsp1407I 821 TGTACA T^GTACA
BspEI 2874 TCCGGA T^CCGGA
BspHI 1622 TCATGA T^CATGA
BspLU11I 3216 ACATGT A^CATGT
BsrGI 821 TGTACA T^GTACA
BstAPI 2649 GCAATGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRI 3415 GAATTC G^AATTC
EcoT22I 3123 ATGCAT ATGCA^T
FbaI 3140 TGATCA T^GATCA
HincII 3544 GTTAAC GT[CT]^[GA]AC
HindII 3544 GTTAAC GT[CT]^[GA]AC
HindIII 718 AAGCTT A^AGCTT
HpaI 3544 GTTAAC GTT^AAC
MamI 2977 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2874 TCCGGA T^CCGGA
NaeI 290 GCCGGC GCC^GGC
NcoI 1280 CCATGG C^CATGG
NgoMIV 290 GCCGGC G^CCGGC
NsiI 3123 ATGCAT ATGCA^T
PpuMI 2346 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3610 ATTAAT AT^TAAT
RcaI 1622 TCATGA T^CATGA
SexAI 988 ACCAGGT A^CC[AT]GGT
SspBI 821 TGTACA T^GTACA
SspI 3573 AATATT AAT^ATT
StuI 1717 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 1515 GTGCAC G^TGCAC
2921 GTGCAC
AocI 300 CCTCAGG CC^T[ACGT]AGG
1154 CCTGAGG
ApaLI 1515 GTGCAC G^TGCAC
2921 GTGCAC
Asp700I 687 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2427 GAAGAGATTC
AvaI 679 CTCGGG C^[CT]CG[GA]G
2380 CCCGAG
BalI 140 TGGCCA TGG^CCA
1444 TGGCCA
BbvCI 663 GCTGAGG CCTCAGC(-5/-2)
1230 GCTGAGG
BglI 92 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
290 GCCGGCCGGGC
BglII 736 AGATCT A^GATCT
3027 AGATCT
BsoBI 679 CTCGGG C^[CT]CG[GA]G
2380 CCCGAG
BspMI 3173 ACCTGC ACCTGC(4/8)
16 GCAGGT
BsrFI 290 GCCGGC [GA]^CCGG[CT]
1519 ACCGGC
Bsu36I 300 CCTCAGG CC^T[ACGT]AGG
1154 CCTGAGG
Cfr10I 290 GCCGGC [GA]^CCGG[CT]
1519 ACCGGC
DsaI 35 CCACGG C^C[GA][CT]GG
1280 CCATGG
Eco81I 300 CCTCAGG CC^T[ACGT]AGG
1154 CCTGAGG
EcoNI 1154 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2886 CCTCAGTAAGG
FauI 408 CCCGC CCCGC(4/6)
3034 GCGGG
HaeII 306 GGCGCT [GA]GCGC^[CT]
428 GGCGCT
MluNI 140 TGGCCA TGG^CCA
1444 TGGCCA
MscI 140 TGGCCA TGG^CCA
1444 TGGCCA
NspI 1532 GCATGT [GA]CATG^[CT]
3216 ACATGT
SacI 23 GAGCTC GAGCT^C
480 GAGCTC
SpeI 965 ACTAGT A^CTAGT
2175 ACTAGT
TaqI 1076 TCGA T^CGA
2045 TCGA
TthHB8I 1076 TCGA T^CGA
2045 TCGA
XcmI 9 CCAGCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1280 CCATGGGCCTCTTGG
XmnI 687 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2427 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII