################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AgeI AscI Asp718I AviII AvrII BalI BamHI BbeI BcgI BglI BglII BlnI BsaHI BsaWI BseAI BsiEI BsiWI BspEI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CpoI EaeI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MluI MluNI MroI MscI MvnI NarI NheI NotI NruI NsiI NspV PacI PinAI PmeI PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SphI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 2153 AACGTT AA^CGTT AflII 3704 CTTAAG C^TTAAG AflIII 80 ACGTGT A^C[GA][CT]GT Alw44I 3257 GTGCAC G^TGCAC Aor51HI 4790 AGCGCT AGC^GCT ApaI 24 GGGCCC GGGCC^C ApaLI 3257 GTGCAC G^TGCAC AseI 4182 ATTAAT AT^TAAT AsnI 4182 ATTAAT AT^TAAT AspI 3321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 4728 CACGTG CAC^GTG BbvCI 4476 GCTGAGG CCTCAGC(-5/-2) BcnI 4773 CCCGG CC^[GC]GG BfrI 3704 CTTAAG C^TTAAG BsaBI 620 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 937 GAGACG CGTCTC(1/5) BspDI 912 ATCGAT AT^CGAT BsrBI 4746 GAGCGG CCGCTC(-3/-3) BsrDI 3546 CATTGC GCAATG(2/0) BsrFI 901 GCCGGC [GA]^CCGG[CT] BstXI 4260 CCAAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 901 GCCGGC [GA]^CCGG[CT] ClaI 912 ATCGAT AT^CGAT DraIII 3694 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 4790 AGCGCT AGC^GCT EcoRI 3177 GAATTC G^AATTC FauI 1156 CCCGC CCCGC(4/6) HindIII 1315 AAGCTT A^AGCTT MamI 620 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2845 CAATTG C^AATTG MunI 2845 CAATTG C^AATTG NaeI 901 GCCGGC GCC^GGC NciI 4773 CCCGG CC^[GC]GG NgoMIV 901 GCCGGC G^CCGGC PflFI 3321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4703 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 4728 CACGTG CAC^GTG PmlI 4728 CACGTG CAC^GTG PshAI 1141 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4182 ATTAAT AT^TAAT Psp1406I 2153 AACGTT AA^CGTT SapI 318 GCTCTTC GCTCTTC(1/4) SbfI 2983 CCTGCAGG CCTGCA^GG SnaBI 3762 TACGTA TAC^GTA Sse8387I 2983 CCTGCAGG CCTGCA^GG SspI 1038 AATATT AAT^ATT Tth111I 3321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4703 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3689 CCAGGCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AocI 1712 CCTGAGG CC^T[ACGT]AGG 4513 CCTCAGG AvaI 652 CTCGAG C^[CT]CG[GA]G 3077 CTCGAG BclI 3941 TGATCA T^GATCA 4089 TGATCA BsaI 3379 GGTCTC GGTCTC(1/5) 4380 GGTCTC BsmI 3307 GAATGC GAATGC(1/-1) 4588 GAATGC BsoBI 652 CTCGAG C^[CT]CG[GA]G 3077 CTCGAG BspHI 1688 TCATGA T^CATGA 4384 TCATGA Bsu36I 1712 CCTGAGG CC^T[ACGT]AGG 4513 CCTCAGG DrdI 1547 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4142 GACATCAGGGTC Eco81I 1712 CCTGAGG CC^T[ACGT]AGG 4513 CCTCAGG EcoNI 1743 CCTGTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2953 CCTCTATGAGG EcoT14I 1476 CCATGG C^C[AT][AT]GG 2747 CCATGG FbaI 3941 TGATCA T^GATCA 4089 TGATCA HaeII 1622 GGCGCT [GA]GCGC^[CT] 4790 AGCGCT HapII 902 CCGG C^CGG 4774 CCGG HgaI 471 GACGC GACGC(5/10) 1077 GACGC HpaII 902 CCGG C^CGG 4774 CCGG MspA1I 3220 CAGCTG C[AC]G^C[GT]G 3729 CAGCTG MspI 902 CCGG C^CGG 4774 CCGG NcoI 1476 CCATGG C^CATGG 2747 CCATGG NspI 2207 ACATGC [GA]CATG^[CT] 2860 ACATGC PaeR7I 652 CTCGAG C^TCGAG 3077 CTCGAG PvuII 3220 CAGCTG CAG^CTG 3729 CAGCTG RcaI 1688 TCATGA T^CATGA 4384 TCATGA SacI 1514 GAGCTC GAGCT^C 3724 GAGCTC SpeI 1503 ACTAGT A^CTAGT 1936 ACTAGT StyI 1476 CCATGG C^C[AT][AT]GG 2747 CCATGG XhoI 652 CTCGAG C^TCGAG 3077 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI Asp700I AspEI AspHI AvaII BanI BanII BbsI BbvI BciVI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI Eam1105I EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI