##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AgeI AscI Asp718I AviII
AvrII BalI BamHI BbeI BcgI
BglI BglII BlnI BsaHI BsaWI
BseAI BsiEI BsiWI BspEI BspLU11I
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CpoI
EaeI EagI EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI FspI Hin1I
HpaI KasI KpnI KspI MluI
MluNI MroI MscI MvnI NarI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SphI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 2153 AACGTT AA^CGTT
AflII 3704 CTTAAG C^TTAAG
AflIII 80 ACGTGT A^C[GA][CT]GT
Alw44I 3257 GTGCAC G^TGCAC
Aor51HI 4790 AGCGCT AGC^GCT
ApaI 24 GGGCCC GGGCC^C
ApaLI 3257 GTGCAC G^TGCAC
AseI 4182 ATTAAT AT^TAAT
AsnI 4182 ATTAAT AT^TAAT
AspI 3321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4728 CACGTG CAC^GTG
BbvCI 4476 GCTGAGG CCTCAGC(-5/-2)
BcnI 4773 CCCGG CC^[GC]GG
BfrI 3704 CTTAAG C^TTAAG
BsaBI 620 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 937 GAGACG CGTCTC(1/5)
BspDI 912 ATCGAT AT^CGAT
BsrBI 4746 GAGCGG CCGCTC(-3/-3)
BsrDI 3546 CATTGC GCAATG(2/0)
BsrFI 901 GCCGGC [GA]^CCGG[CT]
BstXI 4260 CCAAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 901 GCCGGC [GA]^CCGG[CT]
ClaI 912 ATCGAT AT^CGAT
DraIII 3694 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4790 AGCGCT AGC^GCT
EcoRI 3177 GAATTC G^AATTC
FauI 1156 CCCGC CCCGC(4/6)
HindIII 1315 AAGCTT A^AGCTT
MamI 620 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2845 CAATTG C^AATTG
MunI 2845 CAATTG C^AATTG
NaeI 901 GCCGGC GCC^GGC
NciI 4773 CCCGG CC^[GC]GG
NgoMIV 901 GCCGGC G^CCGGC
PflFI 3321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4703 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4728 CACGTG CAC^GTG
PmlI 4728 CACGTG CAC^GTG
PshAI 1141 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4182 ATTAAT AT^TAAT
Psp1406I 2153 AACGTT AA^CGTT
SapI 318 GCTCTTC GCTCTTC(1/4)
SbfI 2983 CCTGCAGG CCTGCA^GG
SnaBI 3762 TACGTA TAC^GTA
Sse8387I 2983 CCTGCAGG CCTGCA^GG
SspI 1038 AATATT AAT^ATT
Tth111I 3321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4703 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3689 CCAGGCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AocI 1712 CCTGAGG CC^T[ACGT]AGG
4513 CCTCAGG
AvaI 652 CTCGAG C^[CT]CG[GA]G
3077 CTCGAG
BclI 3941 TGATCA T^GATCA
4089 TGATCA
BsaI 3379 GGTCTC GGTCTC(1/5)
4380 GGTCTC
BsmI 3307 GAATGC GAATGC(1/-1)
4588 GAATGC
BsoBI 652 CTCGAG C^[CT]CG[GA]G
3077 CTCGAG
BspHI 1688 TCATGA T^CATGA
4384 TCATGA
Bsu36I 1712 CCTGAGG CC^T[ACGT]AGG
4513 CCTCAGG
DrdI 1547 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4142 GACATCAGGGTC
Eco81I 1712 CCTGAGG CC^T[ACGT]AGG
4513 CCTCAGG
EcoNI 1743 CCTGTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2953 CCTCTATGAGG
EcoT14I 1476 CCATGG C^C[AT][AT]GG
2747 CCATGG
FbaI 3941 TGATCA T^GATCA
4089 TGATCA
HaeII 1622 GGCGCT [GA]GCGC^[CT]
4790 AGCGCT
HapII 902 CCGG C^CGG
4774 CCGG
HgaI 471 GACGC GACGC(5/10)
1077 GACGC
HpaII 902 CCGG C^CGG
4774 CCGG
MspA1I 3220 CAGCTG C[AC]G^C[GT]G
3729 CAGCTG
MspI 902 CCGG C^CGG
4774 CCGG
NcoI 1476 CCATGG C^CATGG
2747 CCATGG
NspI 2207 ACATGC [GA]CATG^[CT]
2860 ACATGC
PaeR7I 652 CTCGAG C^TCGAG
3077 CTCGAG
PvuII 3220 CAGCTG CAG^CTG
3729 CAGCTG
RcaI 1688 TCATGA T^CATGA
4384 TCATGA
SacI 1514 GAGCTC GAGCT^C
3724 GAGCTC
SpeI 1503 ACTAGT A^CTAGT
1936 ACTAGT
StyI 1476 CCATGG C^C[AT][AT]GG
2747 CCATGG
XhoI 652 CTCGAG C^TCGAG
3077 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI Asp700I AspEI
AspHI AvaII BanI BanII BbsI
BbvI BciVI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
Eam1105I EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI