##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AviII BfrI BlpI
Bpu1102I BsaBI BsiWI BsmBI BspDI
BssSI Bst1107I BstZ17I CelII ClaI
CpoI DraI EcoRV FseI FspI
HpaI KspI MamI MfeI MluI
MunI NotI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SapI SexAI
SfiI SgrAI SnaBI SpeI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2523 GGTACC G^GTACC
AccIII 2473 TCCGGA T^CCGGA
Aor51HI 2782 AGCGCT AGC^GCT
Asp718I 2523 GGTACC G^GTACC
AvrII 1883 CCTAGG C^CTAGG
BbrPI 3828 CACGTG CAC^GTG
BcgI 3748 GCACCCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2947 AGATCT A^GATCT
BlnI 1883 CCTAGG C^CTAGG
BseAI 2473 TCCGGA T^CCGGA
BspEI 2473 TCCGGA T^CCGGA
BssHII 352 GCGCGC G^CGCGC
BstBI 801 TTCGAA TT^CGAA
DraIII 3138 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4234 CCATGG C^C[GA][CT]GG
EagI 72 CGGCCG C^GGCCG
EclXI 72 CGGCCG C^GGCCG
Eco47III 2782 AGCGCT AGC^GCT
Eco52I 72 CGGCCG C^GGCCG
EcoRI 492 GAATTC G^AATTC
EcoT22I 3130 ATGCAT ATGCA^T
KpnI 2523 GGTACC GGTAC^C
MroI 2473 TCCGGA T^CCGGA
NaeI 3987 GCCGGC GCC^GGC
NcoI 4234 CCATGG C^CATGG
NdeI 870 CATATG CA^TATG
NgoMIV 3987 GCCGGC G^CCGGC
NheI 4706 GCTAGC G^CTAGC
NruI 63 TCGCGA TCG^CGA
NsiI 3130 ATGCAT ATGCA^T
NspV 801 TTCGAA TT^CGAA
PmaCI 3828 CACGTG CAC^GTG
PmlI 3828 CACGTG CAC^GTG
SalI 1743 GTCGAC G^TCGAC
ScaI 1371 AGTACT AGT^ACT
SfuI 801 TTCGAA TT^CGAA
SphI 3177 GCATGC GCATG^C
SspI 508 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 835 ACATGT A^C[GA][CT]GT
2452 ACATGT
AhdI 39 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
936 GACATCGTGTC
AocI 1857 CCTAAGG CC^T[ACGT]AGG
3681 CCTGAGG
Asp700I 566 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2735 GAAAGTATTC
AspEI 39 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
936 GACATCGTGTC
AspI 1531 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4476 GACCGTGTC
BamHI 1142 GGATCC G^GATCC
2476 GGATCC
BbeI 163 GGCGCC GGCGC^C
213 GGCGCC
BbvCI 51 CCTCAGC CCTCAGC(-5/-2)
1735 CCTCAGC
BclI 3151 TGATCA T^GATCA
3832 TGATCA
BsaAI 1813 CACGTA [CT]AC^GT[GA]
3828 CACGTG
BsaWI 545 TCCGGT [AT]^CCGG[AT]
2473 TCCGGA
BsiEI 72 CGGCCG CG[GA][CT]^CG
1741 CGGTCG
BspHI 1164 TCATGA T^CATGA
3148 TCATGA
BspLU11I 835 ACATGT A^CATGT
2452 ACATGT
BstAPI 2078 GCAGGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3971 GCAGGGGCTGC
BstEII 2585 GGTGACC G^GT[ACGT]ACC
2676 GGTGACC
BstPI 2585 GGTGACC G^GT[ACGT]ACC
2676 GGTGACC
Bsu36I 1857 CCTAAGG CC^T[ACGT]AGG
3681 CCTGAGG
DrdI 2588 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3609 GACAGCCCTGTC
Eam1105I 39 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
936 GACATCGTGTC
Eco81I 1857 CCTAAGG CC^T[ACGT]AGG
3681 CCTGAGG
EcoO65I 2585 GGTGACC G^GT[ACGT]ACC
2676 GGTGACC
FbaI 3151 TGATCA T^GATCA
3832 TGATCA
HgaI 3863 GACGC GACGC(5/10)
4731 GCGTC
HindIII 805 AAGCTT A^AGCTT
2838 AAGCTT
KasI 163 GGCGCC G^GCGCC
213 GGCGCC
MaeII 1814 ACGT A^CGT
3829 ACGT
NarI 163 GGCGCC GG^CGCC
213 GGCGCC
PflFI 1531 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4476 GACCGTGTC
PflMI 3137 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4436 CCAGGTCCTGG
RcaI 1164 TCATGA T^CATGA
3148 TCATGA
SbfI 1545 CCTGCAGG CCTGCA^GG
3371 CCTGCAGG
SfoI 163 GGCGCC GGC^GCC
213 GGCGCC
SmaI 34 CCCGGG CCC^GGG
3019 CCCGGG
Sse8387I 1545 CCTGCAGG CCTGCA^GG
3371 CCTGCAGG
Tth111I 1531 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4476 GACCGTGTC
Van91I 3137 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4436 CCAGGTCCTGG
XmaCI 34 CCCGGG C^CCGGG
3019 CCCGGG
XmaI 34 CCCGGG C^CCGGG
3019 CCCGGG
XmnI 566 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2735 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII