################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BbsI BcgI BlnI BpuAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HaeII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 2055 GACGCC G[GA]^CG[CT]C AflII 3594 CTTAAG C^TTAAG AocI 945 CCTGAGG CC^T[ACGT]AGG Asp700I 2481 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 290 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 1759 GGCACC G^G[CT][GA]CC BbrPI 1369 CACGTG CAC^GTG BbvCI 1592 CCTCAGC CCTCAGC(-5/-2) BciVI 487 GTATCC GTATCC(6/5) BclI 447 TGATCA T^GATCA BfrI 3594 CTTAAG C^TTAAG BglI 1415 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1369 CACGTG [CT]AC^GT[GA] BsaBI 3453 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2055 GACGCC G[GA]^CG[CT]C BspHI 1965 TCATGA T^CATGA BspLU11I 3354 ACATGT A^CATGT BsrBI 2518 CCGCTC CCGCTC(-3/-3) BstAPI 1081 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 225 GGTGACC G^GT[ACGT]ACC BstPI 225 GGTGACC G^GT[ACGT]ACC BstXI 38 CCATCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 945 CCTGAGG CC^T[ACGT]AGG CfoI 2234 GCGC GCG^C DraIII 917 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1630 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 945 CCTGAGG CC^T[ACGT]AGG EcoNI 3010 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 225 GGTGACC G^GT[ACGT]ACC FauI 745 CCCGC CCCGC(4/6) FbaI 447 TGATCA T^GATCA HgaI 2055 GACGC GACGC(5/10) HhaI 2234 GCGC GCG^C Hin1I 2055 GACGCC G[GA]^CG[CT]C HinP1I 2234 GCGC G^CGC HincII 2889 GTTGAC GT[CT]^[GA]AC HindII 2889 GTTGAC GT[CT]^[GA]AC MamI 3453 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflFI 290 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1369 CACGTG CAC^GTG PmlI 1369 CACGTG CAC^GTG RcaI 1965 TCATGA T^CATGA SacI 477 GAGCTC GAGCT^C SexAI 918 ACCAGGT A^CC[AT]GGT SmaI 3211 CCCGGG CCC^GGG StuI 898 AGGCCT AGG^CCT SwaI 2610 ATTTAAAT ATTT^AAAT TaqI 1302 TCGA T^CGA Tth111I 290 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1302 TCGA T^CGA XmaCI 3211 CCCGGG C^CCGGG XmaI 3211 CCCGGG C^CCGGG XmnI 2481 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 813 GTCTAC GT^[AC][GT]AC 2628 GTAGAC AcsI 2744 AAATTT [GA]^AATT[CT] 3004 AAATTT AflIII 1370 ACGTGT A^C[GA][CT]GT 3354 ACATGT Alw44I 152 GTGCAC G^TGCAC 389 GTGCAC ApaLI 152 GTGCAC G^TGCAC 389 GTGCAC ApoI 2744 AAATTT [GA]^AATT[CT] 3004 AAATTT AvaI 665 CCCGAG C^[CT]CG[GA]G 3211 CCCGGG BglII 1853 AGATCT A^GATCT 3402 AGATCT BsoBI 665 CCCGAG C^[CT]CG[GA]G 3211 CCCGGG BspMI 1808 ACCTGC ACCTGC(4/8) 222 GCAGGT BsrDI 2294 CATTGC GCAATG(2/0) 1913 GCAATG DsaI 230 CCATGG C^C[GA][CT]GG 1323 CCATGG NcoI 230 CCATGG C^CATGG 1323 CCATGG PflMI 1692 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1763 CCAAGCTCTGG SapI 3200 GAAGAGC GCTCTTC(1/4) 479 GCTCTTC SspI 2907 AATATT AAT^ATT 2992 AATATT TfiI 2089 GAATC G^A[AT]TC 2433 GAATC Van91I 1692 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1763 CCAAGCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BamHI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI StyI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII