##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbsI BcgI BlnI
BpuAI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcyI 2055 GACGCC G[GA]^CG[CT]C
AflII 3594 CTTAAG C^TTAAG
AocI 945 CCTGAGG CC^T[ACGT]AGG
Asp700I 2481 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 290 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1759 GGCACC G^G[CT][GA]CC
BbrPI 1369 CACGTG CAC^GTG
BbvCI 1592 CCTCAGC CCTCAGC(-5/-2)
BciVI 487 GTATCC GTATCC(6/5)
BclI 447 TGATCA T^GATCA
BfrI 3594 CTTAAG C^TTAAG
BglI 1415 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1369 CACGTG [CT]AC^GT[GA]
BsaBI 3453 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2055 GACGCC G[GA]^CG[CT]C
BspHI 1965 TCATGA T^CATGA
BspLU11I 3354 ACATGT A^CATGT
BsrBI 2518 CCGCTC CCGCTC(-3/-3)
BstAPI 1081 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 225 GGTGACC G^GT[ACGT]ACC
BstPI 225 GGTGACC G^GT[ACGT]ACC
BstXI 38 CCATCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 945 CCTGAGG CC^T[ACGT]AGG
CfoI 2234 GCGC GCG^C
DraIII 917 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1630 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 945 CCTGAGG CC^T[ACGT]AGG
EcoNI 3010 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 225 GGTGACC G^GT[ACGT]ACC
FauI 745 CCCGC CCCGC(4/6)
FbaI 447 TGATCA T^GATCA
HgaI 2055 GACGC GACGC(5/10)
HhaI 2234 GCGC GCG^C
Hin1I 2055 GACGCC G[GA]^CG[CT]C
HinP1I 2234 GCGC G^CGC
HincII 2889 GTTGAC GT[CT]^[GA]AC
HindII 2889 GTTGAC GT[CT]^[GA]AC
MamI 3453 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 290 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1369 CACGTG CAC^GTG
PmlI 1369 CACGTG CAC^GTG
RcaI 1965 TCATGA T^CATGA
SacI 477 GAGCTC GAGCT^C
SexAI 918 ACCAGGT A^CC[AT]GGT
SmaI 3211 CCCGGG CCC^GGG
StuI 898 AGGCCT AGG^CCT
SwaI 2610 ATTTAAAT ATTT^AAAT
TaqI 1302 TCGA T^CGA
Tth111I 290 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1302 TCGA T^CGA
XmaCI 3211 CCCGGG C^CCGGG
XmaI 3211 CCCGGG C^CCGGG
XmnI 2481 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 813 GTCTAC GT^[AC][GT]AC
2628 GTAGAC
AcsI 2744 AAATTT [GA]^AATT[CT]
3004 AAATTT
AflIII 1370 ACGTGT A^C[GA][CT]GT
3354 ACATGT
Alw44I 152 GTGCAC G^TGCAC
389 GTGCAC
ApaLI 152 GTGCAC G^TGCAC
389 GTGCAC
ApoI 2744 AAATTT [GA]^AATT[CT]
3004 AAATTT
AvaI 665 CCCGAG C^[CT]CG[GA]G
3211 CCCGGG
BglII 1853 AGATCT A^GATCT
3402 AGATCT
BsoBI 665 CCCGAG C^[CT]CG[GA]G
3211 CCCGGG
BspMI 1808 ACCTGC ACCTGC(4/8)
222 GCAGGT
BsrDI 2294 CATTGC GCAATG(2/0)
1913 GCAATG
DsaI 230 CCATGG C^C[GA][CT]GG
1323 CCATGG
NcoI 230 CCATGG C^CATGG
1323 CCATGG
PflMI 1692 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCAAGCTCTGG
SapI 3200 GAAGAGC GCTCTTC(1/4)
479 GCTCTTC
SspI 2907 AATATT AAT^ATT
2992 AATATT
TfiI 2089 GAATC G^A[AT]TC
2433 GAATC
Van91I 1692 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCAAGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BamHI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII