################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AflII AflIII AhdI AscI Asp700I Asp718I AspEI BamHI BcgI BciVI BclI BfrI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PmeI PshAI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 3349 GTCTAC GT^[AC][GT]AC AccIII 46 TCCGGA T^CCGGA AclI 1421 AACGTT AA^CGTT AcsI 1975 GAATTT [GA]^AATT[CT] AgeI 107 ACCGGT A^CCGGT Alw44I 1177 GTGCAC G^TGCAC AocI 737 CCTAAGG CC^T[ACGT]AGG ApaI 659 GGGCCC GGGCC^C ApaLI 1177 GTGCAC G^TGCAC ApoI 1975 GAATTT [GA]^AATT[CT] AseI 1712 ATTAAT AT^TAAT AsnI 1712 ATTAAT AT^TAAT AvrII 3434 CCTAGG C^CTAGG BbrPI 2374 CACGTG CAC^GTG BglII 2017 AGATCT A^GATCT BlnI 3434 CCTAGG C^CTAGG BlpI 1060 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1060 GCTGAGC GC^T[ACGT]AGC BseAI 46 TCCGGA T^CCGGA BsmBI 1046 CGTCTC CGTCTC(1/5) BspEI 46 TCCGGA T^CCGGA BsrDI 1683 GCAATG GCAATG(2/0) BstAPI 959 GCACCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 737 CCTAAGG CC^T[ACGT]AGG CelII 1060 GCTGAGC GC^T[ACGT]AGC DraIII 3146 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4170 GACTTTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 737 CCTAAGG CC^T[ACGT]AGG EcoRV 3717 GATATC GAT^ATC HgaI 581 GCGTC GACGC(5/10) KspI 2486 CCGCGG CCGC^GG MroI 46 TCCGGA T^CCGGA PinAI 107 ACCGGT A^CCGGT PmaCI 2374 CACGTG CAC^GTG PmlI 2374 CACGTG CAC^GTG PshBI 1712 ATTAAT AT^TAAT Psp1406I 1421 AACGTT AA^CGTT PvuI 3075 CGATCG CGAT^CG SacII 2486 CCGCGG CCGC^GG SpeI 173 ACTAGT A^CTAGT SspI 4320 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Aor51HI 314 AGCGCT AGC^GCT 1191 AGCGCT AspI 1238 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1672 GACCTTGTC BalI 2241 TGGCCA TGG^CCA 2307 TGGCCA BbvCI 1818 CCTCAGC CCTCAGC(-5/-2) 1445 GCTGAGG BsaAI 2374 CACGTG [CT]AC^GT[GA] 3543 TACGTG BsaI 1208 GAGACC GGTCTC(1/5) 3293 GAGACC BssSI 3225 CACGAG CACGAG(-5/-1) 588 CTCGTG BstEII 1270 GGTGACC G^GT[ACGT]ACC 4372 GGTTACC BstPI 1270 GGTGACC G^GT[ACGT]ACC 4372 GGTTACC BstXI 3656 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4336 CCAGGTAATTGG Eco47III 314 AGCGCT AGC^GCT 1191 AGCGCT EcoNI 377 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 650 CCTGGTCCAGG EcoO65I 1270 GGTGACC G^GT[ACGT]ACC 4372 GGTTACC MluNI 2241 TGGCCA TGG^CCA 2307 TGGCCA MscI 2241 TGGCCA TGG^CCA 2307 TGGCCA PflFI 1238 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1672 GACCTTGTC SexAI 864 ACCAGGT A^CC[AT]GGT 4335 ACCAGGT SmaI 79 CCCGGG CCC^GGG 2956 CCCGGG Tth111I 1238 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1672 GACCTTGTC XmaCI 79 CCCGGG C^CCGGG 2956 CCCGGG XmaI 79 CCCGGG C^CCGGG 2956 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII AviII BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII