##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AflII       AflIII      AhdI        
AscI        Asp700I     Asp718I     AspEI       BamHI       
BcgI        BciVI       BclI        BfrI        BsaBI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        Eam1105I    
EcoRI       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PmeI        PshAI       RcaI        RsrII       
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SphI        Sse8387I    
SspBI       StuI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         3349  GTCTAC               GT^[AC][GT]AC
AccIII         46  TCCGGA               T^CCGGA
AclI         1421  AACGTT               AA^CGTT
AcsI         1975  GAATTT               [GA]^AATT[CT]
AgeI          107  ACCGGT               A^CCGGT
Alw44I       1177  GTGCAC               G^TGCAC
AocI          737  CCTAAGG              CC^T[ACGT]AGG
ApaI          659  GGGCCC               GGGCC^C
ApaLI        1177  GTGCAC               G^TGCAC
ApoI         1975  GAATTT               [GA]^AATT[CT]
AseI         1712  ATTAAT               AT^TAAT
AsnI         1712  ATTAAT               AT^TAAT
AvrII        3434  CCTAGG               C^CTAGG
BbrPI        2374  CACGTG               CAC^GTG
BglII        2017  AGATCT               A^GATCT
BlnI         3434  CCTAGG               C^CTAGG
BlpI         1060  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1060  GCTGAGC              GC^T[ACGT]AGC
BseAI          46  TCCGGA               T^CCGGA
BsmBI        1046  CGTCTC               CGTCTC(1/5)
BspEI          46  TCCGGA               T^CCGGA
BsrDI        1683  GCAATG               GCAATG(2/0)
BstAPI        959  GCACCCGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        737  CCTAAGG              CC^T[ACGT]AGG
CelII        1060  GCTGAGC              GC^T[ACGT]AGC
DraIII       3146  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4170  GACTTTAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        737  CCTAAGG              CC^T[ACGT]AGG
EcoRV        3717  GATATC               GAT^ATC
HgaI          581  GCGTC                GACGC(5/10)
KspI         2486  CCGCGG               CCGC^GG
MroI           46  TCCGGA               T^CCGGA
PinAI         107  ACCGGT               A^CCGGT
PmaCI        2374  CACGTG               CAC^GTG
PmlI         2374  CACGTG               CAC^GTG
PshBI        1712  ATTAAT               AT^TAAT
Psp1406I     1421  AACGTT               AA^CGTT
PvuI         3075  CGATCG               CGAT^CG
SacII        2486  CCGCGG               CCGC^GG
SpeI          173  ACTAGT               A^CTAGT
SspI         4320  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Aor51HI       314  AGCGCT               AGC^GCT
             1191  AGCGCT              
AspI         1238  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1672  GACCTTGTC           
BalI         2241  TGGCCA               TGG^CCA
             2307  TGGCCA              
BbvCI        1818  CCTCAGC              CCTCAGC(-5/-2)
             1445  GCTGAGG             
BsaAI        2374  CACGTG               [CT]AC^GT[GA]
             3543  TACGTG              
BsaI         1208  GAGACC               GGTCTC(1/5)
             3293  GAGACC              
BssSI        3225  CACGAG               CACGAG(-5/-1)
              588  CTCGTG              
BstEII       1270  GGTGACC              G^GT[ACGT]ACC
             4372  GGTTACC             
BstPI        1270  GGTGACC              G^GT[ACGT]ACC
             4372  GGTTACC             
BstXI        3656  CCATGGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4336  CCAGGTAATTGG        
Eco47III      314  AGCGCT               AGC^GCT
             1191  AGCGCT              
EcoNI         377  CCTCGGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              650  CCTGGTCCAGG         
EcoO65I      1270  GGTGACC              G^GT[ACGT]ACC
             4372  GGTTACC             
MluNI        2241  TGGCCA               TGG^CCA
             2307  TGGCCA              
MscI         2241  TGGCCA               TGG^CCA
             2307  TGGCCA              
PflFI        1238  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1672  GACCTTGTC           
SexAI         864  ACCAGGT              A^CC[AT]GGT
             4335  ACCAGGT             
SmaI           79  CCCGGG               CCC^GGG
             2956  CCCGGG              
Tth111I      1238  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1672  GACCTTGTC           
XmaCI          79  CCCGGG               C^CCGGG
             2956  CCCGGG              
XmaI           79  CCCGGG               C^CCGGG
             2956  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
AviII       BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       PaeR7I      PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoI        
XhoII