##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AflII AflIII AhdI
AscI Asp700I Asp718I AspEI BamHI
BcgI BciVI BclI BfrI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eam1105I
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PmeI PshAI RcaI RsrII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI StuI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 3349 GTCTAC GT^[AC][GT]AC
AccIII 46 TCCGGA T^CCGGA
AclI 1421 AACGTT AA^CGTT
AcsI 1975 GAATTT [GA]^AATT[CT]
AgeI 107 ACCGGT A^CCGGT
Alw44I 1177 GTGCAC G^TGCAC
AocI 737 CCTAAGG CC^T[ACGT]AGG
ApaI 659 GGGCCC GGGCC^C
ApaLI 1177 GTGCAC G^TGCAC
ApoI 1975 GAATTT [GA]^AATT[CT]
AseI 1712 ATTAAT AT^TAAT
AsnI 1712 ATTAAT AT^TAAT
AvrII 3434 CCTAGG C^CTAGG
BbrPI 2374 CACGTG CAC^GTG
BglII 2017 AGATCT A^GATCT
BlnI 3434 CCTAGG C^CTAGG
BlpI 1060 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1060 GCTGAGC GC^T[ACGT]AGC
BseAI 46 TCCGGA T^CCGGA
BsmBI 1046 CGTCTC CGTCTC(1/5)
BspEI 46 TCCGGA T^CCGGA
BsrDI 1683 GCAATG GCAATG(2/0)
BstAPI 959 GCACCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 737 CCTAAGG CC^T[ACGT]AGG
CelII 1060 GCTGAGC GC^T[ACGT]AGC
DraIII 3146 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4170 GACTTTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 737 CCTAAGG CC^T[ACGT]AGG
EcoRV 3717 GATATC GAT^ATC
HgaI 581 GCGTC GACGC(5/10)
KspI 2486 CCGCGG CCGC^GG
MroI 46 TCCGGA T^CCGGA
PinAI 107 ACCGGT A^CCGGT
PmaCI 2374 CACGTG CAC^GTG
PmlI 2374 CACGTG CAC^GTG
PshBI 1712 ATTAAT AT^TAAT
Psp1406I 1421 AACGTT AA^CGTT
PvuI 3075 CGATCG CGAT^CG
SacII 2486 CCGCGG CCGC^GG
SpeI 173 ACTAGT A^CTAGT
SspI 4320 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Aor51HI 314 AGCGCT AGC^GCT
1191 AGCGCT
AspI 1238 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1672 GACCTTGTC
BalI 2241 TGGCCA TGG^CCA
2307 TGGCCA
BbvCI 1818 CCTCAGC CCTCAGC(-5/-2)
1445 GCTGAGG
BsaAI 2374 CACGTG [CT]AC^GT[GA]
3543 TACGTG
BsaI 1208 GAGACC GGTCTC(1/5)
3293 GAGACC
BssSI 3225 CACGAG CACGAG(-5/-1)
588 CTCGTG
BstEII 1270 GGTGACC G^GT[ACGT]ACC
4372 GGTTACC
BstPI 1270 GGTGACC G^GT[ACGT]ACC
4372 GGTTACC
BstXI 3656 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4336 CCAGGTAATTGG
Eco47III 314 AGCGCT AGC^GCT
1191 AGCGCT
EcoNI 377 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
650 CCTGGTCCAGG
EcoO65I 1270 GGTGACC G^GT[ACGT]ACC
4372 GGTTACC
MluNI 2241 TGGCCA TGG^CCA
2307 TGGCCA
MscI 2241 TGGCCA TGG^CCA
2307 TGGCCA
PflFI 1238 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1672 GACCTTGTC
SexAI 864 ACCAGGT A^CC[AT]GGT
4335 ACCAGGT
SmaI 79 CCCGGG CCC^GGG
2956 CCCGGG
Tth111I 1238 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1672 GACCTTGTC
XmaCI 79 CCCGGG C^CCGGG
2956 CCCGGG
XmaI 79 CCCGGG C^CCGGG
2956 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
AviII BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII