##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI Alw44I ApaLI AscI
Asp700I AspI AviII BbeI BclI
BsiEI BsiWI BspDI BspLU11I BssHII
ClaI CpoI EagI EclXI Eco52I
EcoRI EcoT22I FbaI FseI FspI
HpaI KasI MfeI MluI MunI
NarI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PmeI PshAI
Psp1406I PvuI RsrII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 982 TCCGGA T^CCGGA
AcyI 954 GGCGTC G[GA]^CG[CT]C
AflII 3531 CTTAAG C^TTAAG
AocI 2181 CCTGAGG CC^T[ACGT]AGG
AvaI 1931 CTCGGG C^[CT]CG[GA]G
BamHI 2622 GGATCC G^GATCC
BbvCI 2451 CCTCAGC CCTCAGC(-5/-2)
BfrI 3531 CTTAAG C^TTAAG
BglI 4477 GCCTGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 548 AGATCT A^GATCT
BsaHI 954 GGCGTC G[GA]^CG[CT]C
BseAI 982 TCCGGA T^CCGGA
BsoBI 1931 CTCGGG C^[CT]CG[GA]G
BspEI 982 TCCGGA T^CCGGA
BssSI 2615 CACGAG CACGAG(-5/-1)
Bst1107I 3763 GTATAC GTA^TAC
BstAPI 2795 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4648 TTCGAA TT^CGAA
BstZ17I 3763 GTATAC GTA^TAC
Bsu36I 2181 CCTGAGG CC^T[ACGT]AGG
DraI 1314 TTTAAA TTT^AAA
DraIII 2214 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3953 GACATTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2181 CCTGAGG CC^T[ACGT]AGG
EcoNI 464 CCTAGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4601 GATATC GAT^ATC
Hin1I 954 GGCGTC G[GA]^CG[CT]C
HincII 612 GTTGAC GT[CT]^[GA]AC
HindII 612 GTTGAC GT[CT]^[GA]AC
KspI 375 CCGCGG CCGC^GG
MroI 982 TCCGGA T^CCGGA
NheI 936 GCTAGC G^CTAGC
NspV 4648 TTCGAA TT^CGAA
PvuII 3272 CAGCTG CAG^CTG
SacI 2726 GAGCTC GAGCT^C
SacII 375 CCGCGG CCGC^GG
SapI 1985 GCTCTTC GCTCTTC(1/4)
SfuI 4648 TTCGAA TT^CGAA
SpeI 3635 ACTAGT A^CTAGT
SphI 3672 GCATGC GCATG^C
SspI 4627 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2186 GGTACC G^GTACC
3489 GGTACC
AflIII 2821 ACGTGT A^C[GA][CT]GT
3086 ACACGT
AgeI 872 ACCGGT A^CCGGT
3391 ACCGGT
AseI 564 ATTAAT AT^TAAT
990 ATTAAT
AsnI 564 ATTAAT AT^TAAT
990 ATTAAT
Asp718I 2186 GGTACC G^GTACC
3489 GGTACC
AvrII 1418 CCTAGG C^CTAGG
3119 CCTAGG
BbrPI 2799 CACGTG CAC^GTG
3087 CACGTG
BcgI 2026 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2297 GCAGAAGTCTCG
BlnI 1418 CCTAGG C^CTAGG
3119 CCTAGG
BsaBI 522 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2196 GATTCTGATC
BsmBI 338 CGTCTC CGTCTC(1/5)
694 GAGACG
BsmI 1589 GAATGC GAATGC(1/-1)
1940 GAATGC
BspHI 1022 TCATGA T^CATGA
2402 TCATGA
BspMI 682 ACCTGC ACCTGC(4/8)
2889 GCAGGT
BsrDI 1771 GCAATG GCAATG(2/0)
2839 GCAATG
HgaI 203 GACGC GACGC(5/10)
955 GCGTC
HindIII 2380 AAGCTT A^AGCTT
4555 AAGCTT
KpnI 2186 GGTACC GGTAC^C
3489 GGTACC
MamI 522 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2196 GATTCTGATC
NaeI 172 GCCGGC GCC^GGC
3404 GCCGGC
NcoI 2084 CCATGG C^CATGG
2755 CCATGG
NgoMIV 172 GCCGGC G^CCGGC
3404 GCCGGC
PinAI 872 ACCGGT A^CCGGT
3391 ACCGGT
PmaCI 2799 CACGTG CAC^GTG
3087 CACGTG
PmlI 2799 CACGTG CAC^GTG
3087 CACGTG
PpuMI 289 AGGACCT [GA]G^G[AT]CC[CT]
3153 GGGACCC
PshBI 564 ATTAAT AT^TAAT
990 ATTAAT
PstI 1882 CTGCAG CTGCA^G
2793 CTGCAG
RcaI 1022 TCATGA T^CATGA
2402 TCATGA
StuI 2950 AGGCCT AGG^CCT
3012 AGGCCT
TaqI 2771 TCGA T^CGA
4649 TCGA
TthHB8I 2771 TCGA T^CGA
4649 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AhdI AluI AlwI AlwNI Aor51HI
ApaI ApoI AspEI AspHI AvaII
BalI BanI BanII BbsI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrBI BsrFI BsrGI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco47III EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII