################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AclI Alw44I ApaLI AscI Asp700I AspI AviII BbeI BclI BsiEI BsiWI BspDI BspLU11I BssHII ClaI CpoI EagI EclXI Eco52I EcoRI EcoT22I FbaI FseI FspI HpaI KasI MfeI MluI MunI NarI NdeI NotI NruI NsiI PacI PaeR7I PflFI PmeI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 982 TCCGGA T^CCGGA AcyI 954 GGCGTC G[GA]^CG[CT]C AflII 3531 CTTAAG C^TTAAG AocI 2181 CCTGAGG CC^T[ACGT]AGG AvaI 1931 CTCGGG C^[CT]CG[GA]G BamHI 2622 GGATCC G^GATCC BbvCI 2451 CCTCAGC CCTCAGC(-5/-2) BfrI 3531 CTTAAG C^TTAAG BglI 4477 GCCTGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 548 AGATCT A^GATCT BsaHI 954 GGCGTC G[GA]^CG[CT]C BseAI 982 TCCGGA T^CCGGA BsoBI 1931 CTCGGG C^[CT]CG[GA]G BspEI 982 TCCGGA T^CCGGA BssSI 2615 CACGAG CACGAG(-5/-1) Bst1107I 3763 GTATAC GTA^TAC BstAPI 2795 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4648 TTCGAA TT^CGAA BstZ17I 3763 GTATAC GTA^TAC Bsu36I 2181 CCTGAGG CC^T[ACGT]AGG DraI 1314 TTTAAA TTT^AAA DraIII 2214 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3953 GACATTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2181 CCTGAGG CC^T[ACGT]AGG EcoNI 464 CCTAGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 4601 GATATC GAT^ATC Hin1I 954 GGCGTC G[GA]^CG[CT]C HincII 612 GTTGAC GT[CT]^[GA]AC HindII 612 GTTGAC GT[CT]^[GA]AC KspI 375 CCGCGG CCGC^GG MroI 982 TCCGGA T^CCGGA NheI 936 GCTAGC G^CTAGC NspV 4648 TTCGAA TT^CGAA PvuII 3272 CAGCTG CAG^CTG SacI 2726 GAGCTC GAGCT^C SacII 375 CCGCGG CCGC^GG SapI 1985 GCTCTTC GCTCTTC(1/4) SfuI 4648 TTCGAA TT^CGAA SpeI 3635 ACTAGT A^CTAGT SphI 3672 GCATGC GCATG^C SspI 4627 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 2186 GGTACC G^GTACC 3489 GGTACC AflIII 2821 ACGTGT A^C[GA][CT]GT 3086 ACACGT AgeI 872 ACCGGT A^CCGGT 3391 ACCGGT AseI 564 ATTAAT AT^TAAT 990 ATTAAT AsnI 564 ATTAAT AT^TAAT 990 ATTAAT Asp718I 2186 GGTACC G^GTACC 3489 GGTACC AvrII 1418 CCTAGG C^CTAGG 3119 CCTAGG BbrPI 2799 CACGTG CAC^GTG 3087 CACGTG BcgI 2026 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2297 GCAGAAGTCTCG BlnI 1418 CCTAGG C^CTAGG 3119 CCTAGG BsaBI 522 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2196 GATTCTGATC BsmBI 338 CGTCTC CGTCTC(1/5) 694 GAGACG BsmI 1589 GAATGC GAATGC(1/-1) 1940 GAATGC BspHI 1022 TCATGA T^CATGA 2402 TCATGA BspMI 682 ACCTGC ACCTGC(4/8) 2889 GCAGGT BsrDI 1771 GCAATG GCAATG(2/0) 2839 GCAATG HgaI 203 GACGC GACGC(5/10) 955 GCGTC HindIII 2380 AAGCTT A^AGCTT 4555 AAGCTT KpnI 2186 GGTACC GGTAC^C 3489 GGTACC MamI 522 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2196 GATTCTGATC NaeI 172 GCCGGC GCC^GGC 3404 GCCGGC NcoI 2084 CCATGG C^CATGG 2755 CCATGG NgoMIV 172 GCCGGC G^CCGGC 3404 GCCGGC PinAI 872 ACCGGT A^CCGGT 3391 ACCGGT PmaCI 2799 CACGTG CAC^GTG 3087 CACGTG PmlI 2799 CACGTG CAC^GTG 3087 CACGTG PpuMI 289 AGGACCT [GA]G^G[AT]CC[CT] 3153 GGGACCC PshBI 564 ATTAAT AT^TAAT 990 ATTAAT PstI 1882 CTGCAG CTGCA^G 2793 CTGCAG RcaI 1022 TCATGA T^CATGA 2402 TCATGA StuI 2950 AGGCCT AGG^CCT 3012 AGGCCT TaqI 2771 TCGA T^CGA 4649 TCGA TthHB8I 2771 TCGA T^CGA 4649 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AhdI AluI AlwI AlwNI Aor51HI ApaI ApoI AspEI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrBI BsrFI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco47III EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII