##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AocI ApaI AscI Asp700I Asp718I
AspI AviII AvrII BamHI BbrPI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BspDI BspEI
BsrDI Bst1107I BstZ17I Bsu36I CelII
ClaI CpoI DraII Eco81I EcoO109I
EcoRI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NgoMIV NotI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SexAI SfiI SgrAI SmaI SnaBI
SpeI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2655 GTCGAC GT^[AC][GT]AC
AcsI 3804 AAATTT [GA]^AATT[CT]
Alw44I 694 GTGCAC G^TGCAC
Aor51HI 758 AGCGCT AGC^GCT
ApaLI 694 GTGCAC G^TGCAC
ApoI 3804 AAATTT [GA]^AATT[CT]
AseI 3654 ATTAAT AT^TAAT
AsnI 3654 ATTAAT AT^TAAT
AvaI 1701 CTCGGG C^[CT]CG[GA]G
BbeI 26 GGCGCC GGCGC^C
BcgI 521 CGAAGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2494 GTATCC GTATCC(6/5)
BclI 884 TGATCA T^GATCA
BsaAI 2970 CACGTA [CT]AC^GT[GA]
BsiEI 193 CGGCCG CG[GA][CT]^CG
BsoBI 1701 CTCGGG C^[CT]CG[GA]G
BspHI 2186 TCATGA T^CATGA
BspLU11I 139 ACATGT A^CATGT
BssHII 35 GCGCGC G^CGCGC
BssSI 733 CACGAG CACGAG(-5/-1)
EagI 193 CGGCCG C^GGCCG
EclXI 193 CGGCCG C^GGCCG
Eco47III 758 AGCGCT AGC^GCT
Eco52I 193 CGGCCG C^GGCCG
EcoRV 1564 GATATC GAT^ATC
FbaI 884 TGATCA T^GATCA
KasI 26 GGCGCC G^GCGCC
NarI 26 GGCGCC GG^CGCC
NheI 2737 GCTAGC G^CTAGC
NruI 720 TCGCGA TCG^CGA
PacI 3542 TTAATTAA TTAAT^TAA
PshBI 3654 ATTAAT AT^TAAT
RcaI 2186 TCATGA T^CATGA
SacI 1201 GAGCTC GAGCT^C
SalI 2655 GTCGAC G^TCGAC
SbfI 2718 CCTGCAGG CCTGCA^GG
ScaI 3633 AGTACT AGT^ACT
SfoI 26 GGCGCC GGC^GCC
Sse8387I 2718 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 26 GGCGCC G[GA]^CG[CT]C
1051 GACGCC
AhdI 222 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2941 GACTGTGCGTC
AspEI 222 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2941 GACTGTGCGTC
BbsI 522 GAAGAC GAAGAC(2/6)
3208 GAAGAC
BbvCI 127 CCTCAGC CCTCAGC(-5/-2)
2701 CCTCAGC
BglI 2275 GCCGAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2314 GCCAAGAAGGC
BpuAI 522 GAAGAC GAAGAC(2/6)
3208 GAAGAC
BsaHI 26 GGCGCC G[GA]^CG[CT]C
1051 GACGCC
BsmBI 1361 CGTCTC CGTCTC(1/5)
241 GAGACG
Bsp1407I 365 TGTACA T^GTACA
3384 TGTACA
BsrBI 2097 GAGCGG CCGCTC(-3/-3)
3261 GAGCGG
BsrGI 365 TGTACA T^GTACA
3384 TGTACA
BstAPI 163 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1329 GCAGTCTGTGC
BstBI 2873 TTCGAA TT^CGAA
4319 TTCGAA
BstEII 4651 GGTAACC G^GT[ACGT]ACC
4661 GGTAACC
BstPI 4651 GGTAACC G^GT[ACGT]ACC
4661 GGTAACC
BstXI 1307 CCACCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1961 CCATCAAGATGG
DraIII 2920 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4791 CACTCTGTG
DrdI 868 GACCTGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3368 GACTGTTCGGTC
Eam1105I 222 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2941 GACTGTGCGTC
EcoO65I 4651 GGTAACC G^GT[ACGT]ACC
4661 GGTAACC
Hin1I 26 GGCGCC G[GA]^CG[CT]C
1051 GACGCC
NcoI 515 CCATGG C^CATGG
1829 CCATGG
NdeI 880 CATATG CA^TATG
4735 CATATG
NspV 2873 TTCGAA TT^CGAA
4319 TTCGAA
PshAI 1284 GACACCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1770 GACCCTGGTC
SapI 1707 GAAGAGC GCTCTTC(1/4)
2388 GAAGAGC
SfuI 2873 TTCGAA TT^CGAA
4319 TTCGAA
SspBI 365 TGTACA T^GTACA
3384 TGTACA
StuI 166 AGGCCT AGG^CCT
1973 AGGCCT
TfiI 1006 GATTC G^A[AT]TC
2697 GAATC
XcmI 208 CCAGCCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1829 CCATGGAGGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AflIII AgeI
AluI AlwI AlwNI AspHI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoNI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PinAI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII