##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp700I AviII
BbrPI BcgI BciVI BfrI BsaAI
BsaBI BsiWI BsmBI BspDI BssHII
BssSI Bst1107I BstXI BstZ17I ClaI
CpoI EcoNI FseI FspI HindIII
HpaI MamI MluI NcoI NdeI
NheI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1072 GACGTC GACGT^C
AccIII 2083 TCCGGA T^CCGGA
AhdI 687 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2059 AGCGCT AGC^GCT
AspEI 687 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 780 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3565 TGGCCA TGG^CCA
BbeI 44 GGCGCC GGCGC^C
BglI 4207 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 2083 TCCGGA [AT]^CCGG[AT]
BseAI 2083 TCCGGA T^CCGGA
BsgI 1155 CTGCAC GTGCAG(16/14)
Bsp1407I 2444 TGTACA T^GTACA
BspEI 2083 TCCGGA T^CCGGA
BspLU11I 2482 ACATGT A^CATGT
BsrGI 2444 TGTACA T^GTACA
BstAPI 1761 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3159 TTCGAA TT^CGAA
BstEII 2799 GGTAACC G^GT[ACGT]ACC
BstPI 2799 GGTAACC G^GT[ACGT]ACC
DraIII 3607 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2280 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 687 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2059 AGCGCT AGC^GCT
EcoO65I 2799 GGTAACC G^GT[ACGT]ACC
EcoRV 2421 GATATC GAT^ATC
EcoT22I 3164 ATGCAT ATGCA^T
HincII 2740 GTCAAC GT[CT]^[GA]AC
HindII 2740 GTCAAC GT[CT]^[GA]AC
KasI 44 GGCGCC G^GCGCC
KspI 2706 CCGCGG CCGC^GG
MluNI 3565 TGGCCA TGG^CCA
MroI 2083 TCCGGA T^CCGGA
MscI 3565 TGGCCA TGG^CCA
NarI 44 GGCGCC GG^CGCC
NotI 246 GCGGCCGC GC^GGCCGC
NsiI 3164 ATGCAT ATGCA^T
NspV 3159 TTCGAA TT^CGAA
PflFI 780 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 2706 CCGCGG CCGC^GG
SapI 1902 GAAGAGC GCTCTTC(1/4)
SfoI 44 GGCGCC GGC^GCC
SfuI 3159 TTCGAA TT^CGAA
SspBI 2444 TGTACA T^GTACA
SspI 3691 AATATT AAT^ATT
Tth111I 780 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 2027 GGTACC G^GTACC
4251 GGTACC
AccI 786 GTCTAC GT^[AC][GT]AC
2699 GTAGAC
AflIII 1576 ACACGT A^C[GA][CT]GT
2482 ACATGT
Asp718I 2027 GGTACC G^GTACC
4251 GGTACC
AvrII 4774 CCTAGG C^CTAGG
4932 CCTAGG
BbvCI 3436 CCTCAGC CCTCAGC(-5/-2)
255 GCTGAGG
BclI 2404 TGATCA T^GATCA
2432 TGATCA
BglII 2521 AGATCT A^GATCT
4581 AGATCT
BlnI 4774 CCTAGG C^CTAGG
4932 CCTAGG
BspMI 404 GCAGGT ACCTGC(4/8)
944 GCAGGT
BsrBI 1537 GAGCGG CCGCTC(-3/-3)
1719 GAGCGG
EcoRI 158 GAATTC G^AATTC
4302 GAATTC
FbaI 2404 TGATCA T^GATCA
2432 TGATCA
KpnI 2027 GGTACC GGTAC^C
4251 GGTACC
MaeII 1073 ACGT A^CGT
1578 ACGT
MfeI 3126 CAATTG C^AATTG
3724 CAATTG
MunI 3126 CAATTG C^AATTG
3724 CAATTG
PflMI 2044 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3554 CCATCTCCTGG
PshAI 1782 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1956 GACAAGAGTC
SmaI 9 CCCGGG CCC^GGG
111 CCCGGG
Van91I 2044 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3554 CCATCTCCTGG
XcmI 1375 CCAGCGGCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3432 CCAGCCTCAGCCTGG
XmaCI 9 CCCGGG C^CCGGG
111 CCCGGG
XmaI 9 CCCGGG C^CCGGG
111 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII