##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AflIII AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BclI BglI BlnI BsaAI
BsiWI BsmI BspDI BspLU11I Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI KspI MluI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 661 GTCTAC GT^[AC][GT]AC
AccII 4095 CGCG CG^CG
ApaI 2889 GGGCCC GGGCC^C
AseI 3873 ATTAAT AT^TAAT
AsnI 3873 ATTAAT AT^TAAT
BalI 803 TGGCCA TGG^CCA
BbeI 824 GGCGCC GGCGC^C
BciVI 3637 GTATCC GTATCC(6/5)
BlpI 2009 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2009 GCTCAGC GC^T[ACGT]AGC
BsaBI 186 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3152 CGTCTC CGTCTC(1/5)
Bsp1407I 3558 TGTACA T^GTACA
BspHI 2203 TCATGA T^CATGA
BsrBI 2996 CCGCTC CCGCTC(-3/-3)
BsrGI 3558 TGTACA T^GTACA
BssHII 4094 GCGCGC G^CGCGC
BssSI 3610 CTCGTG CACGAG(-5/-1)
BstEII 924 GGTCACC G^GT[ACGT]ACC
BstPI 924 GGTCACC G^GT[ACGT]ACC
BstUI 4095 CGCG CG^CG
BstXI 1045 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2009 GCTCAGC GC^T[ACGT]AGC
EagI 20 CGGCCG C^GGCCG
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
EcoNI 585 CCTCTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 924 GGTCACC G^GT[ACGT]ACC
EcoRI 3817 GAATTC G^AATTC
FauI 2845 GCGGG CCCGC(4/6)
HindIII 1364 AAGCTT A^AGCTT
KasI 824 GGCGCC G^GCGCC
MamI 186 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 463 CAATTG C^AATTG
MluNI 803 TGGCCA TGG^CCA
MscI 803 TGGCCA TGG^CCA
MslI 3628 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 463 CAATTG C^AATTG
MvnI 4095 CGCG CG^CG
NaeI 696 GCCGGC GCC^GGC
NarI 824 GGCGCC GG^CGCC
NdeI 1116 CATATG CA^TATG
NgoMIV 696 GCCGGC G^CCGGC
NspI 3501 GCATGT [GA]CATG^[CT]
PshBI 3873 ATTAAT AT^TAAT
RcaI 2203 TCATGA T^CATGA
SapI 171 GCTCTTC GCTCTTC(1/4)
SfoI 824 GGCGCC GGC^GCC
SspBI 3558 TGTACA T^GTACA
StuI 3594 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 1839 TCCGGA T^CCGGA
2862 TCCGGA
AcyI 824 GGCGCC G[GA]^CG[CT]C
4108 GACGCC
AflII 2321 CTTAAG C^TTAAG
3485 CTTAAG
AgeI 2451 ACCGGT A^CCGGT
3565 ACCGGT
AlwNI 1101 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
3786 CAGTTTCTG
Asp700I 1327 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2661 GAAACTATTC
BamHI 2057 GGATCC G^GATCC
2731 GGATCC
BbvCI 2979 CCTCAGC CCTCAGC(-5/-2)
2243 GCTGAGG
BfrI 2321 CTTAAG C^TTAAG
3485 CTTAAG
BsaHI 824 GGCGCC G[GA]^CG[CT]C
4108 GACGCC
BseAI 1839 TCCGGA T^CCGGA
2862 TCCGGA
BsgI 1550 CTGCAC GTGCAG(16/14)
4116 CTGCAC
BspEI 1839 TCCGGA T^CCGGA
2862 TCCGGA
BspMI 921 GCAGGT ACCTGC(4/8)
4076 GCAGGT
BsrDI 2073 CATTGC GCAATG(2/0)
3336 CATTGC
DraIII 2015 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
3607 CACCTCGTG
HaeII 824 GGCGCC [GA]GCGC^[CT]
3030 GGCGCT
Hin1I 824 GGCGCC G[GA]^CG[CT]C
4108 GACGCC
MroI 1839 TCCGGA T^CCGGA
2862 TCCGGA
PinAI 2451 ACCGGT A^CCGGT
3565 ACCGGT
SacI 790 GAGCTC GAGCT^C
2610 GAGCTC
SfcI 8 CTGTAG C^T[GA][CT]AG
373 CTACAG
SspI 3446 AATATT AAT^ATT
3863 AATATT
XmnI 1327 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2661 GAAACTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI