##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BbrPI
BciVI BclI BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I EcoRI EcoRV FbaI
FseI FspI HpaI KpnI MamI
MluI MroI NaeI NcoI NdeI
NgoMIV NheI NotI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 2254 GTAGAC GT^[AC][GT]AC
AflII 1702 CTTAAG C^TTAAG
AflIII 3324 ACGTGT A^C[GA][CT]GT
AhdI 578 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2397 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3160 AGCGCT AGC^GCT
AspEI 578 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 157 GGCGCC GGCGC^C
BbvCI 1486 GCTGAGG CCTCAGC(-5/-2)
BfrI 1702 CTTAAG C^TTAAG
BsmBI 832 GAGACG CGTCTC(1/5)
Bsp1407I 1649 TGTACA T^GTACA
BsrBI 322 GAGCGG CCGCTC(-3/-3)
BsrGI 1649 TGTACA T^GTACA
Bsu36I 2397 CCTCAGG CC^T[ACGT]AGG
Eam1105I 578 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3160 AGCGCT AGC^GCT
Eco81I 2397 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1204 ATGCAT ATGCA^T
HincII 1278 GTTGAC GT[CT]^[GA]AC
HindII 1278 GTTGAC GT[CT]^[GA]AC
HindIII 3684 AAGCTT A^AGCTT
KasI 157 GGCGCC G^GCGCC
KspI 518 CCGCGG CCGC^GG
MfeI 2632 CAATTG C^AATTG
MunI 2632 CAATTG C^AATTG
NarI 157 GGCGCC GG^CGCC
NruI 89 TCGCGA TCG^CGA
NsiI 1204 ATGCAT ATGCA^T
PaeR7I 3036 CTCGAG C^TCGAG
PshAI 2459 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 518 CCGCGG CCGC^GG
SapI 1005 GCTCTTC GCTCTTC(1/4)
ScaI 1832 AGTACT AGT^ACT
SfiI 1707 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 157 GGCGCC GGC^GCC
SphI 1206 GCATGC GCATG^C
SspBI 1649 TGTACA T^GTACA
TfiI 1885 GATTC G^A[AT]TC
XbaI 2519 TCTAGA T^CTAGA
XhoI 3036 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 760 GTGCAC G^TGCAC
850 GTGCAC
ApaLI 760 GTGCAC G^TGCAC
850 GTGCAC
Asp700I 1956 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2484 GAAGGTCTTC
BamHI 1743 GGATCC G^GATCC
2900 GGATCC
BcgI 165 CGAGCCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
436 CGAGTGCGCTGC
BsmAI 832 GAGAC GTCTC(1/5)
2457 GAGAC
BsrDI 3575 CATTGC GCAATG(2/0)
2687 GCAATG
BsrFI 299 ACCGGC [GA]^CCGG[CT]
731 GCCGGT
Cfr10I 299 ACCGGC [GA]^CCGG[CT]
731 GCCGGT
DraI 1813 TTTAAA TTT^AAA
3701 TTTAAA
DsaI 518 CCGCGG C^C[GA][CT]GG
617 CCACGG
MaeII 584 ACGT A^CGT
3324 ACGT
PflMI 2501 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2890 CCAAGTGCTGG
SacI 93 GAGCTC GAGCT^C
904 GAGCTC
TaqI 1883 TCGA T^CGA
3037 TCGA
TthHB8I 1883 TCGA T^CGA
3037 TCGA
Van91I 2501 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2890 CCAAGTGCTGG
XmnI 1956 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2484 GAAGGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmaCI XmaI