################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AgeI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BbrPI BciVI BclI BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoRI EcoRV FbaI FseI FspI HpaI KpnI MamI MluI MroI NaeI NcoI NdeI NgoMIV NheI NotI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 2254 GTAGAC GT^[AC][GT]AC AflII 1702 CTTAAG C^TTAAG AflIII 3324 ACGTGT A^C[GA][CT]GT AhdI 578 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2397 CCTCAGG CC^T[ACGT]AGG Aor51HI 3160 AGCGCT AGC^GCT AspEI 578 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 157 GGCGCC GGCGC^C BbvCI 1486 GCTGAGG CCTCAGC(-5/-2) BfrI 1702 CTTAAG C^TTAAG BsmBI 832 GAGACG CGTCTC(1/5) Bsp1407I 1649 TGTACA T^GTACA BsrBI 322 GAGCGG CCGCTC(-3/-3) BsrGI 1649 TGTACA T^GTACA Bsu36I 2397 CCTCAGG CC^T[ACGT]AGG Eam1105I 578 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3160 AGCGCT AGC^GCT Eco81I 2397 CCTCAGG CC^T[ACGT]AGG EcoT22I 1204 ATGCAT ATGCA^T HincII 1278 GTTGAC GT[CT]^[GA]AC HindII 1278 GTTGAC GT[CT]^[GA]AC HindIII 3684 AAGCTT A^AGCTT KasI 157 GGCGCC G^GCGCC KspI 518 CCGCGG CCGC^GG MfeI 2632 CAATTG C^AATTG MunI 2632 CAATTG C^AATTG NarI 157 GGCGCC GG^CGCC NruI 89 TCGCGA TCG^CGA NsiI 1204 ATGCAT ATGCA^T PaeR7I 3036 CTCGAG C^TCGAG PshAI 2459 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 518 CCGCGG CCGC^GG SapI 1005 GCTCTTC GCTCTTC(1/4) ScaI 1832 AGTACT AGT^ACT SfiI 1707 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 157 GGCGCC GGC^GCC SphI 1206 GCATGC GCATG^C SspBI 1649 TGTACA T^GTACA TfiI 1885 GATTC G^A[AT]TC XbaI 2519 TCTAGA T^CTAGA XhoI 3036 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Alw44I 760 GTGCAC G^TGCAC 850 GTGCAC ApaLI 760 GTGCAC G^TGCAC 850 GTGCAC Asp700I 1956 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2484 GAAGGTCTTC BamHI 1743 GGATCC G^GATCC 2900 GGATCC BcgI 165 CGAGCCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 436 CGAGTGCGCTGC BsmAI 832 GAGAC GTCTC(1/5) 2457 GAGAC BsrDI 3575 CATTGC GCAATG(2/0) 2687 GCAATG BsrFI 299 ACCGGC [GA]^CCGG[CT] 731 GCCGGT Cfr10I 299 ACCGGC [GA]^CCGG[CT] 731 GCCGGT DraI 1813 TTTAAA TTT^AAA 3701 TTTAAA DsaI 518 CCGCGG C^C[GA][CT]GG 617 CCACGG MaeII 584 ACGT A^CGT 3324 ACGT PflMI 2501 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2890 CCAAGTGCTGG SacI 93 GAGCTC GAGCT^C 904 GAGCTC TaqI 1883 TCGA T^CGA 3037 TCGA TthHB8I 1883 TCGA T^CGA 3037 TCGA Van91I 2501 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2890 CCAAGTGCTGG XmnI 1956 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2484 GAAGGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI